Correlative light and electron microscopy
Fluorescence light microscopy provides analytical, quantitative, and three-dimensional data with emphasis on analysis of live cells using fluorescent markers. The advent of fluorescent protein probes has further revolutionized fluorescence microscopy by allowing visualization of any protein of interest. However, fluorescence microscopy has limited resolution and only labeled targets can be seen.
On the other hand, electron microscopy achieves the highest definition in cellular ultrastructure, but with a small field of view and not in living cells.
Correlative light and electron microscopy (CLEM) bridges the resolution gap between light microscopy and electron microscopy, thus enabling fluorescence imaging that reveals protein localization and function to be placed in the context of cellular ultrastructure.
There are many ways for doing CLEM. If you are interested in applying CLEM in your research, contact the Advanced Microscopy Facility for consultation and we will help you design and carry out the experiment.