2011-2016

Publications (2011-2016)

39. Z. Chen, and H-w. Ai*, Single Fluorescent Protein-Based Indicators for Zinc Ion (Zn2+)Anal. Chem., 2016, 88: 9029-9036.

ZnGreen

38. S. Youssef, W. Ren, and H-w. Ai*, A Genetically Encoded FRET Sensor for Hypoxia and Prolyl HydroxylasesACS Chem. Biol., 2016, 11: 2492-2498.

ProCY

37. Z. Chen, Z. Tian, K. Kallio, A.L. Oleson, A. Ji, D. Brochardt, D. Jiang, S.J. Remington*, and H-w. Ai*, The N-B Interaction through a Water Bridge: Understanding the Chemoselectivity of a Fluorescent Protein Based Probe for PeroxynitriteJ. Am. Chem. Soc., 2016, 138: 4900-4907.

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36. W. Ren, and H-w. Ai*, Photocontrol of the Src Kinase in Mammalian Cells with a Photocaged InteinMeth. Mol. Biol. 2016, 1495: DOI 10.1007/978-1-4939-6451-2_14 (Book chapter in Henning Mootz ed., Split Inteins: Methods and Protocols).

35. Y. Fan, and H-w. Ai*, Development of redox-sensitive red fluorescent proteins for imaging redox dynamics in cellular compartmentsAnal. Bioanal. Chem., 2016, 408: 2901-2911. Selected by the Editor as a “Paper in Forefront”.

ABC_redox

34. W. Ren, A. Ji, O. Karmach, D. Carter, M. Martins-Green*, and H-w. Ai*, A Membrane-Activatable Near-Infrared Fluorescent Probe with Ultra-Photostability for Imaging Mitochondrial Membrane PotentialAnalyst, 2016,141, 3679-3685.

figure34a

33. W. Ren, A. Ji, M.X. Wang, and H-w. Ai*, “Expanding the Genetic Code for a Dinitrophenyl Hapten“, ChemBioChem, 2015, 16: 2007-2010.

hapten

32. W. Ren, T.M. Truong and H-w. Ai*, “Study of the Binding Energies between Unnatural Amino Acids and Engineered Orthogonal Tyrosyl-tRNA Synthetases“, Sci. Rep., 2015, 5: 12632.

mmmd

31. Y. Fan,# Z. Chen,# and H-w. Ai*, “Monitoring Redox Dynamics in Living Cells with a Redox-Sensitive Red Fluorescent Protein“, Anal. Chem., 2015, 87: 2802-2810. (#equal contribution)

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30. W. Ren,# A. Ji,# and H-w. Ai*, “Light Activation of Protein Splicing with a Photocaged Fast Intein“, J. Am. Chem. Soc., 2015, 137: 2155-2158. (#equal contribution)

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29. Z. Chen, T. Truong and H-w. Ai*, “Development of Fluorescent Probes for the Detection of Peroxynitrite“, in Peroxynitrite Detection in Biological Media: Challenges and Advances. Royal Society of Chemistry (RSC) Publishing. Print publication date: Oct. 21, 2015.

figure29a

28. H-w. Ai*, “Fluorescent protein-based probes: General principles and practices“, Anal. Bioanal. Chem., 2015, 407: 9-15.

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27. H-w. Ai*, “Editorial: Fluorescent Sensors for Biological Applications“, Sensors, 2014, 14: 17829-17831.

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26. Z. Chen and H-w. Ai*, “A Highly Responsive and Selective Fluorescent Probe to Image Physiological Hydrogen Sulfide“, Biochemistry, 2014, 53: 5966-5974.

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25. A. Ji,# W. Ren,# and H-w. Ai*, “A Highly Efficient Oxidative Condensation Reaction for Selective Protein conjugation“, Chem. Commun, 2014, 50: 7469-7472 (#equal contribution)

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24. H-w. Ai, M.A. Baird, Y. Shen, M.W. Davidson and R.E. Campbell*, “Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications“, Nature Protocols, 2014, 9: 910-928.

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23. W. Ren, and H-w. Ai*, “Genetically encoded fluorescent redox probes“, Sensors, 2013, 13: 15422-15433.

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22. Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, “Genetically Encoded Fluorescent Probe for the Selective Detection of Peroxynitrite“, J. Am. Chem. Soc., 2013, 135: 14940-14943.

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21. A. Chatterjee, H. Xiao, M. Bollong, H-w. Ai and P.G. Schultz*, “Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells“, Proc. Natl. Acad. Sci. USA., 2013, 110: 11803-11808.

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20. S. Chen, Z. Chen, W. Ren and H-w. Ai*, “Reaction-based genetically encoded fluorescent hydrogen sulfide sensors“, J. Am. Chem. Soc., 2012, 134: 9589-9592.

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19. W. Ren and H-w. Ai*, “Ribosomal Incorporation of Unusual Amino Acids: Learning from Mother Nature“, book chapter in Ribosomes: Molecular Structure, Role in Biological Functions and Implications for Genetic Diseases. Nova Science Publishers. Published on May 3, 2013.

figure19a

18. H-w. Ai*, “Biochemical analysis with the expanded genetic lexicon“, Anal. Bioanal. Chem., 2012, 403: 2089-2102. In the special issue featuring “Young Investigators in Analytical and Bioanalytical Science”.

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Publications during undergraduate, graduate, and postdoctoral training