Combinatorial Antibody Libraries background
Features of antibody libraries
- Antibody libraries allow screening of the natural repertoire of antibodies in a system where genotype and phenotype are linked in a package capable of replication. See phage display and yeast display.
- Antibody libraries eliminate immunization and the use of live animals in generating antibodies.
- Antibody libraries circumvent immune tolerance, which is important because most therapeutic antibodies are designed to recognize self-antigens.
- In vitro selection methods allow the user to customize selection conditions such as buffer, pH, temperature, and competitor antigens.
- In vitro selection methods eliminate antigen proteolysis, clearance and auto-antigen selection that occur in an animal setting.
- The in vitro surface display methods allow for the rapid isolation of antibodies against infectious pathogens and their toxins that are impractical within in vivo systems, such as hybridomas.