Combinatorial Antibody Libraries background

Features of antibody libraries

  1. Antibody libraries allow screening of the natural repertoire of antibodies in a system where  genotype and phenotype are linked in a package capable of replication. See phage display and yeast display.
  2. Antibody libraries eliminate  immunization and the use of live animals in generating antibodies.
  3. Antibody libraries circumvent immune tolerance, which is important because most therapeutic antibodies are designed to recognize self-antigens.
  4. In vitro selection methods allow the user to customize selection conditions such as buffer, pH, temperature, and competitor antigens.
  5. In vitro selection methods eliminate antigen proteolysis, clearance and auto-antigen selection that occur in an animal setting.
  6. The in vitro surface display methods allow for the rapid isolation of antibodies against infectious pathogens and their toxins that are impractical within in vivo systems, such as hybridomas.