Combinatorial antibody libraries
Combinatorial antibody library
I. Human naïve antibody library (scFv)
Human monoclonal antibody discovery by phage display is well established as a robust and reliable source for the generation of antibodies for therapeutics and diagnostics applications.
Human naïve antibody libraries are derived from natural, unimmunized, rearranged V genes (e.g., from the IgM B-cell pool). Naïve human phage antibody library is comprised of antibody fragments from a source of genes that is not explicitly biased to contain clones binding to antigens; intrinsically they are useful for selecting antibodies against all possible types of antigen structures.
AbET core has constructed human naïve antibody phage display library in single chain fragment variable (scFv) format, with a library having more than 1.2 x 1010 clones that cover the human antibody repertoire.
Key features of Human Antibody Libraries
|Selection and screening of human monoclonal antibodies||Generation of entirely human antibody sequences
4-5 weeks from existing libraries (for screening & clonal expansion of human antibody candidate
100-500 µg antigen required
|Options for antigens used for screening||
Targets that are not normally seen because of the natural immune response
|Antigen immobilization for screening|
|Affinity||Range is nM to µM:|
|Alternative antibody formats||Beause the genetic information encoding the antibody is immediately available, the antibody can immediately be produced in other formats if required, e.g. IgG, scFv-Fc fusion including whole-antibody monoclonal isotypes (IgG, IgA, IgE) compatible with bacterial and other expression systems.|