Antibody purification

Mouse monoclonal Antibody purification productAbET core purifies antibodies by standard chromatography modes.

For mouse monoclonal antibodies produced by hybridoma technology, protein G or protein A columns, which bind to the fragment crystallizable region (FC) provide high purity antibodies.

Other modes are:

  • ion exchange
  • gel filtration.

The core has two systems;

  • biocompatible Shimadzu HPLC, which uses inert titanium rather than stainless steel which is prone to corrosion by chloride ions.
  • An ÄKTA™ pure FPLC (GE Healthcare), funded by Equipment Trust Fund. This instrument is largely metal free having  few small areas exposed to inert titanium.