Antibody purification
AbET core purifies antibodies by standard chromatography modes.
For mouse monoclonal antibodies produced by hybridoma technology, protein G or protein A columns, which bind to the fragment crystallizable region (FC) provide high purity antibodies.
Other modes are:
- ion exchange
- gel filtration.
The core has two systems;
- biocompatible Shimadzu HPLC, which uses inert titanium rather than stainless steel which is prone to corrosion by chloride ions.
- An ÄKTA™ pure FPLC (GE Healthcare), funded by Equipment Trust Fund. This instrument is largely metal free having few small areas exposed to inert titanium.