Instruments in Shared Instrumentation Core

plate-readerMolecular Dynamics Gemini EM SpectraMax Fluorescent plate reader

  • For fluorescence readings of plates with 384, 96, 24, 12, 6 well microtiter plates.
  • Dual monochromator optics.
  • Does not perform absorbance readings.
  • Operated by researcher. Fee for use.

 

DionexHPLCHigh performance liquid chromatography.

  • Dionex Ultimate for 0.3  to 4.6 mm diameter columns.

Variable wavelength UV detector.Used for separation of peptides, proteins and some other molecules,  usually with  reverse phase columns.

 

 

 

 

AVIV-410AVIV 410 Circular dichroism spectropolarimeter

For studies on tertiary structure of proteins.
Operated by researcher. Fee for use.
Brief background on operation here.

 

 

Scanscope-smallAperio ScanScope Slide Scanner

Aperio ScanScope to scan microscope slides at 20X, 40X, 63X (resolutions of 0.5, 0.25, 0.17 micron/pixel) for digital pathology. The scans can be exported as TIFF or JPEG files. The scanner can scan a batch of 5 slides.

 

 

 

GS-800-smallGel and film scanners

  • Bio-Rad GS800 for absorbance readings on gels or blots at visible wavelengths and X-ray film.  Three wavelength filters for different stains. ImageQuant software for image analysis available
  • Bio-Rad FX fluorescent scanner. Three lasers and five filters to cover common fluorescent stains. Multiple stains can be scanned and overlaid.
  • Both scanners produce files which can be converted to 8 or 16 bit tiff files.

 

fortéBIO Octet RED96 uses BioLayer Interferometry with disposable probes to measure association and dissociation rates of pairs of molecules and hence dissociation constants.
This instrument can perform 8 simultaneous readings. It can also perform quantitation measurements including some equivalent to ELISA.

 

RTCAAcea RTCA cell analysis instrument

real time, continuous measurement of cellular activity, including migration, based on impedance measurements

 

AbsorbancereaderBiotek ELx800 absorbance plate reader

absorbance measurements on microtiter plates at 450, 562, 600, 660 nm