Mass spectrometry services

Before starting a project, researchers should discuss the project with Dr. Nicholas Sherman (nes3f@virginia.edu, 924.0070) to ensure that the experimental design and sample preparation are suitable for the available analyses.

  • Protein identification and sequencing from gel or solution by either peptide mass fingerprinting or ESI-LC/MS/MS at the low femtomole level (Simple mixture  MS/MS service).
  • Analysis of protein mixtures such as tissue and media (Complex mixture MS/MS service)
  • protein analysis of samples prepared by Laser Capture Microdissection (BTRF)
  • Proteomics, comparison of proteins in tissue samples using chemical/isotopic labels or label free analysis
  • Absolute quantitation of proteins using labeled peptides and selective reaction monitoring (MRM service)
  • Identification of phosphorylation sites using titanium dioxide enrichment
  • Identification of post-translational modifications (e.g.  ubiquitination) (de novo sequencing service/PTM service)
  • De Novo (manual) sequence analysis of novel proteins to obtain sufficient sequence data for cloning
  • High resolution, high mass accuracy measurements of peptides and small proteins by ESI
  • Mass determination by MALDI mass measurements for confirming identity of peptides and proteins, either by staff or open access self service
  • Identification of interaction partners
  • Quantitative analysis of metabolites, peptides, biomarkers, small molecules by SRM
  • Bioinformatics for large data sets.