{"id":12,"date":"2015-07-04T22:41:07","date_gmt":"2015-07-05T02:41:07","guid":{"rendered":"http:\/\/cores.med.virginia.edutest-replo\/cost-analysis\/"},"modified":"2024-10-17T09:18:16","modified_gmt":"2024-10-17T13:18:16","slug":"equipment","status":"publish","type":"page","link":"https:\/\/med.virginia.edu\/biomolecular-analysis-facility\/equipment\/","title":{"rendered":"Equipment"},"content":{"rendered":"

The Biomolecular Analysis Facility has equipment which is either not commonly found in individual laboratories or is needed only occasionally by some laboratories. Some equipment is operated by facility personnel, and some is used by individual researchers.<\/p>\n

For more information: contact Nicholas Sherman ph. 924-0070<\/p>\n

Statement of resources for grant applications<\/a><\/h4>\n

 <\/p>\n

\u200b<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
TYPE OF EQUIPMENT<\/th>\nIMAGE<\/th>\n<\/tr>\n
\n

Thermo Scientific Q Exactive HF-X<\/h3>\n

We have four methods of loading samples into this instrument:<\/p>\n

    \n
  • infusion pump (no fractionation)<\/li>\n
  • Capillary electrophoresis<\/li>\n
  • nano UHPLC-flow rates of nnn for 75 \u00b5m diamater columns<\/li>\n
  • UHPLCs with flow rates up to 1 ml\/min for larger columns<\/li>\n<\/ul>\n

    The mass spectrometer has an Orbitrap mass analyzer with a mass range of 50 to 8000, and resolution up to 240,000.<\/p>\n

     <\/td>\n

\"q-exactive<\/a><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n
\n

Bruker MicroFlex MALDI<\/h3>\n

This instrument measures masses of intact molecules. Sample preparation is usually relatively simple so this instrument is the first choice to obtain the mass of peptides, proteins and some other molecules.<\/td>\n

\"bruker<\/td>\n<\/tr>\n
\n

Molecular Dynamics Gemini EM SpectraMax Fluorescent plate reader<\/a><\/h3>\n
    \n
  • For fluorescence readings of plates with 384, 96, 24, 12, 6 well microtiter plates.<\/li>\n
  • Dual monochromator optics.<\/li>\n
  • Does not perform absorbance readings.<\/li>\n
  • Operated by researcher. Fee for use.<\/li>\n<\/ul>\n<\/td>\n

\"fluorescence<\/td>\n<\/tr>\n
\n

High performance liquid chromatography.<\/h3>\n
    \n
  • Dionex Ultimate for 0.3 to 4.6 mm diameter columns.<\/li>\n<\/ul>\n

    Variable wavelength UV detector.Used for separation of peptides, proteins and some other molecules, usually with reverse phase columns.<\/td>\n

\"Dionex<\/td>\n<\/tr>\n
\n

JASCO J-1500 Circular dichroism spectropolarimeter<\/h3>\n

For studies on tertiary structure of proteins.
\nOperated by researcher. Fee for use.
\nBrief background on operation here<\/a>.<\/td>\n

\"\"<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n
\n

Molecular interactions<\/h3>\n

fort\u00e9BIO Octet RED96<\/a> uses BioLayer Interferometry with disposable probes to measure association and dissociation rates of pairs of molecules and hence dissociation constants.
\nThis instrument can perform 8 simultaneous readings. It can also perform quantitation measurements including some equivalent to ELISA,<\/td>\n

\"fort\u00e9BIO<\/td>\n<\/tr>\n
\n

Acea RTCA cell analysis instrument<\/a><\/h3>\n

real time, continuous measurement of cellular activity, including migration, based on impedance measurements<\/td>\n

\"ACEA<\/td>\n<\/tr>\n
\n

Biotek ELx800 absorbance plate reader<\/a><\/h3>\n

absorbance measurements on microtiter plates at 450, 562, 600, 660 nm<\/td>\n

\"BioTek<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

The Biomolecular Analysis Facility has equipment which is either not commonly found in individual laboratories or is needed only occasionally by some laboratories. Some equipment is operated by facility personnel, and some is used by individual researchers. For more information: contact Nicholas Sherman ph. 924-0070 Statement of resources for grant applications   \u200b TYPE OF […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":2,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"tags":[],"class_list":["post-12","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nEquipment - Biomolecular Analysis Facility<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/biomolecular-analysis-facility\/equipment\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Equipment - Biomolecular Analysis Facility\" \/>\n<meta property=\"og:description\" content=\"The Biomolecular Analysis Facility has equipment which is either not commonly found in individual laboratories or is needed only occasionally by some laboratories. 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