{"id":107383,"date":"2022-04-18T11:12:01","date_gmt":"2022-04-18T15:12:01","guid":{"rendered":"https:\/\/med.virginia.edu\/cell-biology\/?p=107383"},"modified":"2025-05-15T11:26:58","modified_gmt":"2025-05-15T15:26:58","slug":"characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians","status":"publish","type":"post","link":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/","title":{"rendered":"Characterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians"},"content":{"rendered":"

UVA Author:<\/strong> David Shook
\nCitation:<\/strong> David R Shook, Jason WH Wen, Ana Rolo, Michael O’Hanlon, Brian Francica, Destiny Dobbins, Paul Skoglund, Douglas W DeSimone, Rudolf Winklbauer, Raymond E Keller. eLife, April 2022.<\/p>\n

DOI:<\/strong> https:\/\/elifesciences.org\/articles\/57642<\/a>
\nPub-Med Number<\/strong>: 35404236<\/p>\n

\n
\n

The morphogenic process of convergent thickening (CT) was originally described as the mediolateral convergence and radial thickening of the explanted ventral involuting marginal zone (IMZ) of\u00a0Xenopus<\/i>\u00a0gastrulae (Keller and Danilchik 1988). Here we show that CT is expressed in\u00a0all<\/i>\u00a0sectors of the\u00a0pre-involution<\/i>\u00a0IMZ, which transitions to expressing convergent extension (CE)\u00a0after<\/i>\u00a0involution. CT occurs without CE and drives symmetric blastopore closure in ventralized embryos. Assays of tissue affinity and tissue surface tension measurements suggest CT is driven by increased interfacial tension between the deep IMZ and the overlying epithelium. The resulting minimization of deep IMZ surface area drives a tendency to shorten the mediolateral (circumblastoporal) aspect of the IMZ, thereby generating tensile force contributing to blastopore closure (Shook et al. 2018). These results establish CT as an independent force-generating process of evolutionary significance and provide the first clear example of an oriented, tensile force generated by an isotropic, Holtfreterian\/Steinbergian tissue affinity change.<\/p>\n<\/div>\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

UVA Author: David Shook Citation: David R Shook, Jason WH Wen, Ana Rolo, Michael O’Hanlon, Brian Francica, Destiny Dobbins, Paul Skoglund, Douglas W DeSimone, Rudolf Winklbauer, Raymond E Keller. eLife, April 2022. DOI: https:\/\/elifesciences.org\/articles\/57642 Pub-Med Number: 35404236 The morphogenic process of convergent thickening (CT) was originally described as the mediolateral convergence and radial thickening of […]<\/p>\n","protected":false},"author":1740,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[13],"tags":[],"class_list":["post-107383","post","type-post","status-publish","format-standard","hentry","category-featured-publications"],"acf":false,"yoast_head":"\nCharacterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians - Department of Cell Biology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Characterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians - Department of Cell Biology\" \/>\n<meta property=\"og:description\" content=\"UVA Author: David Shook Citation: David R Shook, Jason WH Wen, Ana Rolo, Michael O’Hanlon, Brian Francica, Destiny Dobbins, Paul Skoglund, Douglas W DeSimone, Rudolf Winklbauer, Raymond E Keller. eLife, April 2022. DOI: https:\/\/elifesciences.org\/articles\/57642 Pub-Med Number: 35404236 The morphogenic process of convergent thickening (CT) was originally described as the mediolateral convergence and radial thickening of […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/\" \/>\n<meta property=\"og:site_name\" content=\"Department of Cell Biology\" \/>\n<meta property=\"article:published_time\" content=\"2022-04-18T15:12:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-05-15T15:26:58+00:00\" \/>\n<meta name=\"author\" content=\"zkr7e@virginia.edu\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"zkr7e@virginia.edu\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/\",\"url\":\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/\",\"name\":\"Characterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians - Department of Cell Biology\",\"isPartOf\":{\"@id\":\"https:\/\/med.virginia.edu\/cell-biology\/#website\"},\"datePublished\":\"2022-04-18T15:12:01+00:00\",\"dateModified\":\"2025-05-15T15:26:58+00:00\",\"author\":{\"@id\":\"https:\/\/med.virginia.edu\/cell-biology\/#\/schema\/person\/b4929a1d4683383a392718e12166cd7f\"},\"breadcrumb\":{\"@id\":\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/med.virginia.edu\/cell-biology\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Characterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/med.virginia.edu\/cell-biology\/#website\",\"url\":\"https:\/\/med.virginia.edu\/cell-biology\/\",\"name\":\"Department of Cell Biology\",\"description\":\"University of Virginia School of Medicine\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/med.virginia.edu\/cell-biology\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/med.virginia.edu\/cell-biology\/#\/schema\/person\/b4929a1d4683383a392718e12166cd7f\",\"name\":\"zkr7e@virginia.edu\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/med.virginia.edu\/cell-biology\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/eb05928a9c10d0bc86fc859e223e3e9cd7a535837367cf67c524db2a42f795d0?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/eb05928a9c10d0bc86fc859e223e3e9cd7a535837367cf67c524db2a42f795d0?s=96&d=mm&r=g\",\"caption\":\"zkr7e@virginia.edu\"},\"url\":\"https:\/\/med.virginia.edu\/cell-biology\/author\/zkr7evirginia-edu\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Characterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians - Department of Cell Biology","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/","og_locale":"en_US","og_type":"article","og_title":"Characterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians - Department of Cell Biology","og_description":"UVA Author: David Shook Citation: David R Shook, Jason WH Wen, Ana Rolo, Michael O’Hanlon, Brian Francica, Destiny Dobbins, Paul Skoglund, Douglas W DeSimone, Rudolf Winklbauer, Raymond E Keller. eLife, April 2022. DOI: https:\/\/elifesciences.org\/articles\/57642 Pub-Med Number: 35404236 The morphogenic process of convergent thickening (CT) was originally described as the mediolateral convergence and radial thickening of […]","og_url":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/","og_site_name":"Department of Cell Biology","article_published_time":"2022-04-18T15:12:01+00:00","article_modified_time":"2025-05-15T15:26:58+00:00","author":"zkr7e@virginia.edu","twitter_card":"summary_large_image","twitter_misc":{"Written by":"zkr7e@virginia.edu","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/","url":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/","name":"Characterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians - Department of Cell Biology","isPartOf":{"@id":"https:\/\/med.virginia.edu\/cell-biology\/#website"},"datePublished":"2022-04-18T15:12:01+00:00","dateModified":"2025-05-15T15:26:58+00:00","author":{"@id":"https:\/\/med.virginia.edu\/cell-biology\/#\/schema\/person\/b4929a1d4683383a392718e12166cd7f"},"breadcrumb":{"@id":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/18\/characterization-of-convergent-thickening-a-major-convergence-force-producing-morphogenic-movement-in-amphibians\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/med.virginia.edu\/cell-biology\/"},{"@type":"ListItem","position":2,"name":"Characterization of convergent thickening, a major convergence force producing morphogenic movement in amphibians"}]},{"@type":"WebSite","@id":"https:\/\/med.virginia.edu\/cell-biology\/#website","url":"https:\/\/med.virginia.edu\/cell-biology\/","name":"Department of Cell Biology","description":"University of Virginia School of Medicine","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/med.virginia.edu\/cell-biology\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/med.virginia.edu\/cell-biology\/#\/schema\/person\/b4929a1d4683383a392718e12166cd7f","name":"zkr7e@virginia.edu","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/med.virginia.edu\/cell-biology\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/eb05928a9c10d0bc86fc859e223e3e9cd7a535837367cf67c524db2a42f795d0?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/eb05928a9c10d0bc86fc859e223e3e9cd7a535837367cf67c524db2a42f795d0?s=96&d=mm&r=g","caption":"zkr7e@virginia.edu"},"url":"https:\/\/med.virginia.edu\/cell-biology\/author\/zkr7evirginia-edu\/"}]}},"_links":{"self":[{"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/posts\/107383","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/users\/1740"}],"replies":[{"embeddable":true,"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/comments?post=107383"}],"version-history":[{"count":1,"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/posts\/107383\/revisions"}],"predecessor-version":[{"id":107452,"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/posts\/107383\/revisions\/107452"}],"wp:attachment":[{"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/media?parent=107383"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/categories?post=107383"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/med.virginia.edu\/cell-biology\/wp-json\/wp\/v2\/tags?post=107383"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}