{"id":107390,"date":"2022-04-21T14:26:57","date_gmt":"2022-04-21T18:26:57","guid":{"rendered":"https:\/\/med.virginia.edu\/cell-biology\/?p=107390"},"modified":"2026-05-21T12:45:08","modified_gmt":"2026-05-21T16:45:08","slug":"biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium","status":"publish","type":"post","link":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/21\/biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium\/","title":{"rendered":"Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium"},"content":{"rendered":"

UVA Author:<\/strong> Gordon Laurie
\nCitation:<\/strong> Lee C, Edman MC, Laurie GW, Hamm-Alvarez SF, MacKay JA. Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium. Int J Mol Sci. 2020;21(17):6157. Published 2020 Aug 26. doi:10.3390\/ijms21176157<\/p>\n

DOI:<\/strong> https:\/\/www.mdpi.com\/1422-0067\/21\/17\/6157<\/a>
\nPub-Med Number<\/strong>: 32859014<\/p>\n

\n
\n

Lacripep is a therapeutic peptide derived from the human tear protein, Lacritin. Lacripep interacts with syndecan-1 and induces mitogenesis upon the removal of heparan sulfates (HS) that are attached at the extracellular domain of syndecan-1. The presence of HS is a prerequisite for the syndecan-1 clustering that stimulates exosome biogenesis and release. Therefore, syndecan-1-mediated mitogenesis versus HS-mediated exosome biogenesis are assumed to be mutually exclusive. This study introduces a biosynthesized fusion between Lacripep and an elastin-like polypeptide named LP-A96, and evaluates its activity on cell motility enhancement versus exosome biogenesis. LP-A96 activates both downstream pathways in a dose-dependent manner. HCE-T cells at high confluence treated with 1 \u03bcM LP-A96 enhanced cell motility equipotent to Lacripep. However, cells at low density treated with 1 \u03bcM LP-A96 generated a 210-fold higher number of exosomes compared to those treated at low density with Lacripep. As monovalent Lacripep is capable of enhancing cell motility but not exosome biogenesis, activation of exosome biogenesis by LP-A96 not only suggests its utility as a novel molecular tool to study the Lacritin biology in the corneal epithelium but also implies activity as a potential therapeutic peptide that can further improve ocular surface health through the induction of exosomes.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

UVA Author: Gordon Laurie Citation: Lee C, Edman MC, Laurie GW, Hamm-Alvarez SF, MacKay JA. Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium. Int J Mol Sci. 2020;21(17):6157. Published 2020 Aug 26. doi:10.3390\/ijms21176157 DOI: https:\/\/www.mdpi.com\/1422-0067\/21\/17\/6157 Pub-Med Number: 32859014 Lacripep is a therapeutic peptide derived from the human tear protein, Lacritin. Lacripep interacts […]<\/p>\n","protected":false},"author":1740,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[13],"tags":[21],"class_list":["post-107390","post","type-post","status-publish","format-standard","hentry","category-featured-publications","tag-delete"],"acf":false,"yoast_head":"\nBiosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium - Department of Cell Biology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/21\/biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium - Department of Cell Biology\" \/>\n<meta property=\"og:description\" content=\"UVA Author: Gordon Laurie Citation: Lee C, Edman MC, Laurie GW, Hamm-Alvarez SF, MacKay JA. Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium. Int J Mol Sci. 2020;21(17):6157. Published 2020 Aug 26. doi:10.3390\/ijms21176157 DOI: https:\/\/www.mdpi.com\/1422-0067\/21\/17\/6157 Pub-Med Number: 32859014 Lacripep is a therapeutic peptide derived from the human tear protein, Lacritin. Lacripep interacts […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/21\/biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium\/\" \/>\n<meta property=\"og:site_name\" content=\"Department of Cell Biology\" \/>\n<meta property=\"article:published_time\" content=\"2022-04-21T18:26:57+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-05-21T16:45:08+00:00\" \/>\n<meta name=\"author\" content=\"zkr7e@virginia.edu\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"zkr7e@virginia.edu\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/med.virginia.edu\\\/cell-biology\\\/2022\\\/04\\\/21\\\/biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/med.virginia.edu\\\/cell-biology\\\/2022\\\/04\\\/21\\\/biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium\\\/\"},\"author\":{\"name\":\"zkr7e@virginia.edu\",\"@id\":\"https:\\\/\\\/med.virginia.edu\\\/cell-biology\\\/#\\\/schema\\\/person\\\/b4929a1d4683383a392718e12166cd7f\"},\"headline\":\"Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium\",\"datePublished\":\"2022-04-21T18:26:57+00:00\",\"dateModified\":\"2026-05-21T16:45:08+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/med.virginia.edu\\\/cell-biology\\\/2022\\\/04\\\/21\\\/biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium\\\/\"},\"wordCount\":252,\"keywords\":[\"Delete\"],\"articleSection\":[\"Featured Publications\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/med.virginia.edu\\\/cell-biology\\\/2022\\\/04\\\/21\\\/biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium\\\/\",\"url\":\"https:\\\/\\\/med.virginia.edu\\\/cell-biology\\\/2022\\\/04\\\/21\\\/biosynthesized-multivalent-lacritin-peptides-stimulate-exosome-production-in-human-corneal-epithelium\\\/\",\"name\":\"Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium - 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