{"id":107403,"date":"2022-04-21T23:12:11","date_gmt":"2022-04-22T03:12:11","guid":{"rendered":"https:\/\/med.virginia.edu\/cell-biology\/?p=107403"},"modified":"2025-05-15T11:33:35","modified_gmt":"2025-05-15T15:33:35","slug":"construction-of-a-mammalian-embryo-model-from-stem-cells-organized-by-a-morphogen-signalling-centre","status":"publish","type":"post","link":"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/21\/construction-of-a-mammalian-embryo-model-from-stem-cells-organized-by-a-morphogen-signalling-centre\/","title":{"rendered":"Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre"},"content":{"rendered":"

UVA Author:<\/strong> Ricardo Moraes Borges
\nCitation:<\/strong> Xu PF, Borges RM, Fillatre J, et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre. Nat Commun. 2021;12(1):3277. Published 2021 Jun 2. doi:10.1038\/s41467-021-23653-4<\/p>\n

DOI:<\/strong> https:\/\/doi.org\/10.1038\/s41467-021-23653-4<\/a>
\nPub-Med Number<\/strong>: 34078907<\/p>\n

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Generating properly differentiated embryonic structures in vitro from pluripotent stem cells remains a challenge. Here we show that instruction of aggregates of mouse embryonic stem cells with an experimentally engineered morphogen signalling centre, that functions as an organizer, results in the development of embryo-like entities (embryoids). In situ hybridization, immunolabelling, cell tracking and transcriptomic analyses show that these embryoids form the three germ layers through a gastrulation process and that they exhibit a wide range of developmental structures, highly similar to neurula-stage mouse embryos. Embryoids are organized around an axial chordamesoderm, with a dorsal neural plate that displays histological properties similar to the murine embryo neuroepithelium and that folds into a neural tube patterned antero-posteriorly from the posterior midbrain to the tip of the tail. Lateral to the chordamesoderm, embryoids display somitic and intermediate mesoderm, with beating cardiac tissue anteriorly and formation of a vasculature network. Ventrally, embryoids differentiate a primitive gut tube, which is patterned both antero-posteriorly and dorso-ventrally. Altogether, embryoids provide an in vitro model of mammalian embryo that displays extensive development of germ layer derivatives and that promises to be a powerful tool for in vitro studies and disease modelling.<\/p>\n<\/div>\n<\/div>\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

UVA Author: Ricardo Moraes Borges Citation: Xu PF, Borges RM, Fillatre J, et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre. Nat Commun. 2021;12(1):3277. Published 2021 Jun 2. doi:10.1038\/s41467-021-23653-4 DOI: https:\/\/doi.org\/10.1038\/s41467-021-23653-4 Pub-Med Number: 34078907 Generating properly differentiated embryonic structures in vitro from pluripotent stem cells remains a […]<\/p>\n","protected":false},"author":1740,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[13],"tags":[],"class_list":["post-107403","post","type-post","status-publish","format-standard","hentry","category-featured-publications"],"acf":false,"yoast_head":"\nConstruction of a mammalian embryo model from stem cells organized by a morphogen signalling centre - Department of Cell Biology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/cell-biology\/2022\/04\/21\/construction-of-a-mammalian-embryo-model-from-stem-cells-organized-by-a-morphogen-signalling-centre\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre - Department of Cell Biology\" \/>\n<meta property=\"og:description\" content=\"UVA Author: Ricardo Moraes Borges Citation: Xu PF, Borges RM, Fillatre J, et al. 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