{"id":107420,"date":"2023-04-21T16:27:01","date_gmt":"2023-04-21T20:27:01","guid":{"rendered":"https:\/\/med.virginia.edu\/cell-biology\/?p=107420"},"modified":"2026-07-09T12:48:03","modified_gmt":"2026-07-09T16:48:03","slug":"septins-guide-noncentrosomal-microtubules-to-promote-focal-adhesion-disassembly-in-migrating-cells","status":"publish","type":"post","link":"https:\/\/med.virginia.edu\/cell-biology\/2023\/04\/21\/septins-guide-noncentrosomal-microtubules-to-promote-focal-adhesion-disassembly-in-migrating-cells\/","title":{"rendered":"Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells"},"content":{"rendered":"

UVA Author:<\/strong> Elias Spiliotis
\nCitation:<\/strong> Merenich D, Nakos K, Pompan T, Donovan SJ, Gill A, Patel P, Spiliotis ET, Myers KA. Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells. Mol Biol Cell. 2022 May 1;33(5):ar40. doi: 10.1091\/mbc.E21-06-0334. Epub 2022 Mar 11. PMID: 35274967; PMCID: PMC9282018.<\/p>\n

DOI:<\/strong> https:\/\/doi.org\/10.1091\/mbc.e21-06-0334<\/a>
\nPub-Med Number<\/strong>: 35274967<\/p>\n

\n
\n
\n

Endothelial cell migration is critical for vascular angiogenesis and is compromised to facilitate tumor metastasis. The migratory process requires the coordinated assembly and disassembly of focal adhesions (FA), actin, and microtubules (MT). MT dynamics at FAs deliver vesicular cargoes and enhance actomyosin contractility to promote FA turnover and facilitate cell advance. Noncentrosomal (NC) MTs regulate FA dynamics and are sufficient to drive cell polarity, but how NC MTs target FAs to control FA turnover is not understood. Here, we show that Rac1 induces the assembly of FA-proximal septin filaments that promote NC MT growth into FAs and inhibit mitotic centromere-associated kinesin (MCAK)-associated MT disassembly, thereby maintaining intact MT plus ends proximal to FAs. Septin-associated MT rescue is coupled with accumulation of Aurora-A kinase and cytoplasmic linker-associated protein (CLASP) localization to the MT between septin and FAs. In this way, NC MTs are strategically positioned to undergo MCAK- and CLASP-regulated bouts of assembly and disassembly into FAs, thereby regulating FA turnover and cell migration.<\/p>\n<\/div>\n<\/div>\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

UVA Author: Elias Spiliotis Citation: Merenich D, Nakos K, Pompan T, Donovan SJ, Gill A, Patel P, Spiliotis ET, Myers KA. Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells. Mol Biol Cell. 2022 May 1;33(5):ar40. doi: 10.1091\/mbc.E21-06-0334. Epub 2022 Mar 11. PMID: 35274967; PMCID: PMC9282018. DOI: https:\/\/doi.org\/10.1091\/mbc.e21-06-0334 Pub-Med Number: 35274967 Endothelial […]<\/p>\n","protected":false},"author":1740,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_members_access_role":[],"_members_access_error":"","_links_to":"","_links_to_target":""},"categories":[13],"tags":[23],"class_list":["post-107420","post","type-post","status-publish","format-standard","hentry","category-featured-publications","tag-department-news"],"acf":false,"yoast_head":"\nSeptins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells - Department of Cell Biology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/cell-biology\/2023\/04\/21\/septins-guide-noncentrosomal-microtubules-to-promote-focal-adhesion-disassembly-in-migrating-cells\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells - Department of Cell Biology\" \/>\n<meta property=\"og:description\" content=\"UVA Author: Elias Spiliotis Citation: Merenich D, Nakos K, Pompan T, Donovan SJ, Gill A, Patel P, Spiliotis ET, Myers KA. Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells. Mol Biol Cell. 2022 May 1;33(5):ar40. doi: 10.1091\/mbc.E21-06-0334. Epub 2022 Mar 11. PMID: 35274967; PMCID: PMC9282018. 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