Equipment in research cores
Major equipment in the School of Medicine research cores
| Equipment | Use | Operated by | Location* | Core |
|---|---|---|---|---|
| Operetta High-Content Imaging System | It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images | Core or Researcher | OMS 4806 | Advanced Microscopy Facility |
| LSM 710 | Core or Researcher | OMS 4806 | Advanced Microscopy Facility | |
| Zeiss Sigma VP HD FE-SEM | Core or Researcher | OMS 4806 | Advanced Microscopy Facility | |
| Zeiss LSM700 confocal microscope | Core or Researcher | OMS 4806 | Advanced Microscopy Facility | |
| LSM 880 Confocal with Airyscan | Reads 10 dyes simultaneously, high sensitivity detector, environmental chamber for live cell imaging | Core or Researcher | OMS 4806 | Advanced Microscopy Facility |
| JOEL JEM-1230 TEM | Core or Researcher | OMS 4806 | Advanced Microscopy Facility | |
| Olympus BX51 | Brightfield, Fluorescence, Phase Contrast and DIC | Core or Researcher | OMS 4806 | Advanced Microscopy Facility |
| Leica Thunder with TIRF | Core or Researcher | OMS 4806 | Advanced Microscopy Facility | |
| Olympus SZX12 dual objective stereo microscope with digital camera | Brightfield, Darkfield and Fluorescence | Core or Researcher | OMS 4806 | Advanced Microscopy Facility |
| High Resolution Vacuum Evaporator for rotary shadowing | metal coating | Core or Researcher | OMS 4806 | Advanced Microscopy Facility |
| Leica Ultracut Ultramicrotomes (3) | Core or Researcher | OMS 4806 | Advanced Microscopy Facility | |
| Critical Point Dryer | Core or Researcher | OMS 4806 | Advanced Microscopy Facility | |
| Sputter Coater | Core or Researcher | OMS 4806 | Advanced Microscopy Facility | |
| fluorescent plate reader | assays using fluorescent reagents in 6 to 384 well plates | researcher | Pinn 1074 (BAF) | |
| circular dichroism spectropolarimeter, JASCO J-1500 | secondary structure of proteins, including melting studies | researcher | Pinn 1074 (BAF) | |
| Bio-Rad GS-800 densitometer | scanning X-ray type films, gels stained with visible stains like Coomassie and silver | researcher | Pinn 1074 (BAF) | |
| Aperio ScanScope | brightfield scanning of microscopy slide to digital images at magnifications of 20X, 40X, 63X for digital pathology | core or researcher | Pinn 1074 (BAF) | |
| Biotek ELx800 absorbance plate reader | absorbance measurements on microtiter plates at 450, 562, 600, 660 nm | researcher | Pinn 1074 (BAF) | |
| Dionex HPLC system for 0.3 to 4.6 mm diameter columns | analysis of small samples of peptides, proteins and other molecules | core | Pinn 1051 BAF) | |
| fortéBIO Octet96 | molecular interactions using BioLayer Interferometry | researcher | Pinn 1074 (BAF) | |
| ImageQuant Tl software | relative quantitation of bands on gels and film | researcher | Pinn 1074 (BAF) | |
| Matrix Assisted Laser Desorption Ionization-Time Of Flight mass spectrometer (MALDI-TOF), Bruker Microflex | measure mass of intact molecules, normally peptides, protein, also small pieces of DNA, other molecules | core or researcher | Pinn 1105 (BAF) | |
| Acea xCELLigence RTCA real time cell analysis instrument | real time, continuous measurement of cellular activity, including migration, based on impedance measurements | researcher | Pinn 1051 BAF) | |
| Thermo Scientific Q Exactive HF-X | identification and sequencing of proteins; proteomics, analysis of other biomolecules | core | Pinn 1105 (BAF) | |
| Computing clusters, dedicated access, 32 cores, 252GB RAM | Computational infrastructure that is essential for analyzing high-throughput biological data | Bioinformatics/maintained by UVA ITS | University Data Center OLD IVY | Bioinformatics |
| Computing clusters, dedicated access, 55 cores, 378GB RAM | Computational infrastructure that is essential for analyzing high-throughput biological data | Bioinformatics/maintained by UVA ITS | University Data Center OLD IVY | Bioinformatics |
| Computing clusters, dedicated access, 72 cores, 378GB RAM | Computational infrastructure that is essential for analyzing high-throughput biological data | Bioinformatics/maintained by UVA ITS | University Data Center OLD IVY | Bioinformatics |
| Bruker Avance III NMR spectrometer 800MHz with HCN Zpfg cryoprobe | Performs wide range of solution NMR experiments | Core or Researcher | Chem 138 | BioNMR |
| Bruker Avance III NMR spectrometer 600MHz with HCN Zpfg probe | Performs wide range of solution NMR experiments | Core or Researcher | Chem 138 | BioNMR |
| Varian NMRS 600 spectrometer with multiple probes | Performs wide range of solution NMR experiments | Core or Researcher | Chem 122 | BioNMR |
| Varian NMRS 600 spectrometer with multiple probes | Performs wide range of solution NMR experiments | Core or Researcher | Chem 175 | BioNMR |
| Varian Inova 500 NMR spectrometer with multiple probes | Performs wide range of solution NMR experiments | Core or Researcher | Chem 175 | BioNMR |
| Arcturus (Life Technologies) XT-TI laser microdissection apparatus | micro scale dissection of fixed or frozen tissue under bright-field, phase contrast or fluorescence visualization | researcher | MR6 B703 | Biorepository & Tissue Research Facility |
| Pathology Devices TMArrayer semi-automated tissue microarray apparatus | Semi-automated construction of tissue microarrays | Core | MR6 G714 | Biorepository & Tissue Research Facility |
| QIAGEN QIAcube robotic workstation | fully automated protein and nucleic acid extraction | Core | MR6 G710 | Biorepository & Tissue Research Facility |
| Next Advance Bullet Blender tissue homogenizer | tissue disruption/homogenization for fresh, frozen or fixed tissue | Core | MR6 G712 | Biorepository & Tissue Research Facility |
| Miltenyi Biotech gentleMACS™ Dissociator | Fresh tissue dissociation for viable cells | Core | MR6 G712 | Biorepository & Tissue Research Facility |
| Bio-Rad iMark Absorbance Microplate Reader | 96-well format plate reader; available filters 415,450,595,620,655,750 nm | Core or Researcher | MR6 G712 | Biorepository & Tissue Research Facility |
| DAKO Autostainer Plus | Automated immunohistochemistry platform | Core | MR6 G710 | Biorepository & Tissue Research Facility |
| DAKO PT Link pretreatment module | Semi-automated depariffinization and antigen retrieval for immunohistochemistry | Core | MR6 G710 | Biorepository & Tissue Research Facility |
| Olympus BX41TF with 12 megapixel Digital Camera and Olympus Microsuite Pathology | Bright-field photomicroscopy and remote collaboration | Core or Researcher | MR6 G712 | Biorepository & Tissue Research Facility |
| Sakura Finetek VIP5 Processor | Automated processing of formalin-fixed tissue | Core | MR6 G714 | Biorepository & Tissue Research Facility |
| Sakura Finetek Tissue-Tek TEC | Preparation of paraffin blocks | Core | MR6 G714 | Biorepository & Tissue Research Facility |
| Microm HM 325 with histocollimator | Microtome for sectioning paraffin blocks and tissue microarrays | Core | MR6 G714 | Biorepository & Tissue Research Facility |
| MVE-Chart vapor phase LN2 freezers | Cryogenic biospecimen storage | Core | MR6 B501B | Biorepository & Tissue Research Facility |
| -30º C freezer | biospecimen storage | Core | MR6 G701A | Biorepository & Tissue Research Facility |
| -80º C freezers | biospecimen storage | Core | MR6 (Multiple Locations) | Biorepository & Tissue Research Facility |
| QIAGEN TissueLyser II | Tissue disruption/homogenization for fresh, frozen or fixed tissue | Core | MR6 G710 | Biorepository & Tissue Research Facility |
| Thermo CryoStarNX50 | Preparation of frozen tissue sections | Core or Researcher | MR6 G714 | Biorepository & Tissue Research Facility |
| Hamamatsu Nanozoomer S360 | Digital pathology: high-throughput whole slide scanning (brightfield) | Core | MR6 G714 | Biorepository & Tissue Research Facility |
| Pathcore Flow image management system | Digital pathology: web-based image storage, collaboration, & sharing | Core or Researcher | MR6 G714 | Biorepository & Tissue Research Facility |
| Visiopharm ONCOTOPIX software | Digital pathology: whole slide, multi-color image analysis | Core | MR6 G714 | Biorepository & Tissue Research Facility |
| Nexcelom Cellometer Auto 2000 Automatic Cell Viability Counter system | Fluorescence-based cell counting | Core | MR6 G712 | Biorepository & Tissue Research Facility |
| Agilent 4150 Tapestation | Labchip-based RNA/DNA quality assessment | Core | MR6 G710 | Biorepository & Tissue Research Facility |
| NanoDrop One Flash Spectrophotometer | Microliter volume direct read quantitation of nucleic acid solutions | Core or Researcher | MR6 G712 | Biorepository & Tissue Research Facility |
| NanoDrop 3300 Fluorospectrometer | Microliter volume dye-based quantitation of nucleic acid solutions | Core or Researcher | MR6 G712 | Biorepository & Tissue Research Facility |
| Ventana Discovery Ultra | Automated immunohistochemistry and chromogenic RNA in-situ platform | Core | MR6 G710 | Biorepository & Tissue Research Facility |
| StatSpin CytoFuge 2 | Creation of cytospin cytology preps | Core | MR6 G710 | Biorepository & Tissue Research Facility |
| 10X Genomics Chromium Controller | Single Cell RNA Seq, single nuclei ATAC-seq and single cell proteogenomics | Core | Pinn 1076 | Genome Analysis Technology Core |
| Illumina MiSeq sequencing system | Small genome sequencing, targeted gene sequencing, 16S metagenomics, and library QC | Core | Pinn 1044 | Genome Analysis Technology Core |
| Illumina NextSeq sequencing system | Broad genomics sequencing applications including RNA-Seq (bulk and single cell), whole exome sequencing, high throughput metagenomics and targeted resequencing | Core | Pinn 1044 | Genome Analysis Technology Core |
| Applied Biosystems Prism QuantStudio 6 Flex Real Time PCR System | Real time PCR for gene expression and genotyping applications. | Core | Pinn 1076 | Genome Analysis Technology Core |
| Agilent Bioanalyzer | Capillary electrophoresis of small samples of DNA and RNA for quality control | Core or Researcher | Pinn 1076 | Genome Analysis Technology Core |
| NanoVue Spectrophotometer | Measuring absorbances of small volumes, especially quantitation of DNA | Core | Pinn 1076 | Genome Analysis Technology Core |
| Qubit fluorometer | Quantitating small samples of DNA and RNA by fluorescence | Core or Researcher | Pinn 1044 | Genome Analysis Technology Core |
| Bio-Rad QX200™ Droplet Digital™ PCR System | Absolute quantitation of DNA and RNA | Core or Researcher | Pinn 1076 | Genome Analysis Technology Core |
| Sage Pippin Prep | Preparation of libraries for NGS using agaroe gel electrophoresis to isolate the desired size(s) | Researcher | Pinn 1076 | Genome Analysis Technology Core |
| Covaris S2 | Shear DNA to sizes suitable for sequencing | Researcher | Pinn 1076 | Genome Analysis Technology Core |
| Agilent Tape Station | QC instrument for RNAs and DNA. It is used to determine RNA integrity number (RIN) and for sizing NGS libraries prior to sequencing | Core or Researcher | Pinn 1076 | Genome Analysis Technology Core |
| Vyaire Vmax | Clinical measurement of oxygen consumption during exercise | Core | Collins 2406 | Exercise Physiology Core Laboratory |
| BOD POD GS Body Composition tracking system (COSMED) | Air displacement plethysmography system for determining body composition, e.g. fat free tissue and fat tissue | Core | Collins 2406 | Exercise Physiology Core Laboratory |
| GE Case Exercise Testing System including ECG and Treadmill | Clinical measurement of oxygen consumption during exercise | Core | Collins 2406 | Exercise Physiology Core Laboratory |
| Monark Arm Crank Ergometer | Upper body ergometer | Core | Collins 2406 | Exercise Physiology Core Laboratory |
| Quinton Q-Stress ECG system | Electrocardiograph for exercise testing; compatible with Lode ergometer | Core | Collins 2406 | Exercise Physiology Core Laboratory |
| Lode Corival Bike | Electronically calibrated research ergometer | Core | Collins 2406 | Exercise Physiology Core Laboratory |
| Brachial Analyzer (MIA Vascular Research Tools 5) software | Medical image analysis software for the assessment of FMD/endothelial function of brachial artery from ultrasound image data. | Core | Collins 2406 | Exercise Physiology Core Laboratory |
| Attune™ NxT | high throughput cytometry with 4 lasers, 14 colors and autosampler | Core | Pinn 2013 (FCCF) | |
| Becton Dickinson FACSVantage SE Turbo Sorter with DIVA cell sorter | 10 color, 4 way sorting, sterile sorting, BSL2 materials, with 3 lasers | Core | Pinn 2013 (FCCF) | |
| Becton Dickinson Fortessa LSRII benchtop analyzer | 4 lasers, 17 detectors at up to 30,000 cells/sec | core or researcher | Pinn 2011 (FCCF) | |
| Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer | 3 laser, 5 color analyses | core or researcher | Pinn 2011 (FCCF) | |
| Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer | 4 laser, 10 color analyses with Automated Micro-Sampler system for 96 well plates | core or researcher | Pinn 2011 (FCCF) | |
| Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer | 2 laser, 4 color analyses | core or researcher | Pinn 2011 (FCCF) | |
| Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer | 2 laser, color analyses | researcher | Pinn 2011 (FCCF) | |
| Amnis ImagestreamX Mark II | imaging cytometer, for dark field, bright field and fluorescent images with 4 lasers, 12 channels, 20X, 40X, 60X magnifications, 96 well plate automation | core or researcher | Pinn 2011 (FCCF) | |
| Luminex MAGPIX® | measures multiple analytes such as chemokines from mouse or human sample | Pinn 2011 (FCCF) | ||
| BD Influx cell sorter for BSL2 samples in custom Baker biosafety cabinet | 6 way sorting of BSL2 samples using 5 lasers, 15 colors | core | Pinn 2013 (FCCF) | |
| FACSAria™ Fusion | sorting of BSL2 samples with 5 lasers, 15 colors, 4 way sorting | (FCCF) | ||
| Fluidigm CyTOF 2 Helios | analysis of multiple parameters at single cell level | core | Pinn 2013 (FCCF) | |
| AutoMACS Pro | Isolation of cell populations labelled with 50 nm superparamagnetic particles conjugated to antibodies to cell surface antigens | researcher | Pinn 2011 (FCCF) | |
| Data analysis work station (5) | 4 Mac stations and 1 PC with FloJo and Modfit software | researcher | Pinn 2011 (FCCF) | |
| Leica DM IRB inverted microscopes | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| Leitz micromanipulators | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| Sutter P-87 micropipette puller | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| De Fonbrune microforge | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| Bio-Cool BCIV40A Controlled-Rate Freezer | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| XYClone laser system | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| NEPA21 type II super electroporator | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| Primetech PMM4G piezo micro manipulator | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| Eppendorf femtojet 4i electronic microinjector | Core | OMS 1845 | Genetically Engineered Murine Model Core | |
| BTX ECM630 Electroporator | Core | Pinn 2223 | Genetically Engineered Murine Model Core | |
| Eppendorf EP Gradient Mastercycler | Core | Pinn 2223 | Genetically Engineered Murine Model Core | |
| UVIdoc LCD Gel Documentation System | Core | Pinn 2223 | Genetically Engineered Murine Model Core | |
| FEI Tecnai™ Spirit | transmission electron microscopy of negatively stained samples, cryo-electron microscopy | researcher | Life Sciences Annex, Snyder (MEMC) | |
| FEI Tecnai™ F20 | cryo-electron microscopy (moderate resolution), high resolution negative stain electron microscopy | researcher | Life Sciences Annex, Snyder (MEMC) | |
| FEI Titan Krios | high resolution cryo-electron microscopy | researcher | Life Sciences Annex, Snyder (MEMC) | |
| FEI Vitrobot | sample vitrification for cryo-transmission electron microscopy | researcher | Life Sciences Annex, Snyder (MEMC) | |
| 7 Tesla Bruker/Siemens ClinScan small animal MRI | MRI scans of small animals | core or research | MR4 1184 (MIC) | |
| Caliper IVIS Spectrum bioluminescence and fluorescence scanner | fluorescence and luminescence scanner for tissue samplesfluorescence and luminescence scanner for tissue samples | core or research | Snyder (MIC) | |
| Bruker Aibira Si trimodal PET/SPCET/CT scanner | scan small animals using positron emission, gamma emission and X-rays with sub-millimeter resolution | Snyder (MIC) | ||
| Siemens Eclipse Cyclotron | production of isotopes for PET scanning | core and Siemens/PETNET | Snyder (MIC) | |
| Radiochemistry modules | synthesis of radiolabels | core | ||
| ultrasound scanner | ultrasound imaging | |||
| Tissue processors for formalin and Bouin’s fixed tissues | core | MR5 1035 (RHC) | ||
| tissue embedding station | MR5 1035 (RHC) | |||
| microtomes | preparing thin sections for microscopy | core | MR5 1035 (RHC) | |
| stainer, automated | staining of tissue sections | core | MR5 1035 (RHC) | |
| cryostat | preparing frozen tissue sections for microscopy | core | MR5 1035 (RHC) | |
| Lonza 4D Nucleofector with X unit | transfection of mammalian cells | Core or Researcher | Pinn 1071 (SCC) | |
| Inverted microscopes, Axio and Olympus | Core or Researcher | Pinn 2034 | Tissue Culture Facility | |
| Olympus IM microscope | Core or Researcher | Pinn 2034 | Tissue Culture Facility | |
| CO2 incubators | Core or Researcher | Pinn 2034 | Tissue Culture Facility | |
| Incubators | Maintenance of insect cell lines | Core | Pinn 2034 | Tissue Culture Facility |
| cold storage, liquid nitrogen | Cell storage | Core | Pinn 2034 | Tissue Culture Facility |
| Biosafety cabinets (2) | Cell culture | Pinn 2034 | Tissue Culture Facility |
Core abbreviations: AMF, Advanced Microscopy Facility; BAF, Biomolecular Analysis Facility; BioNMR, Biomolecular Magnetic Resonance Facility; BTRF, Biorepository and Tissue Research Facility; GATC, Genome Analysis and Technology Core; EPCL, Exercise Physiology Core Laboratory; FCCF, Flow Cytometry Core Facility; GEMM, Genetically Engineered Murine Model Core; MEMC, Molecular Electron Microscopy Core; MIC, Molecular Imaging Core; RHC, Research Histology Core; TCF, SCC, Stem Cell Core; Tissue Culture Facility.*Location information is building name and room number. MSB, Medical School Building. Pinn Hall was formerly called Jordan Hall