Equipment in research cores

Major equipment in the School of Medicine research cores

Equipment Use Operated by Location*
JEOL 1230 transmission electron microscope, real-time digital imaging (2Kx2K), ultra high resolution digital imaging (4Kx4K), film imaging. core or researcher MSB 4806 (AMF)
Zeiss Sigma VP HD field emission SEM scanning electron microscope core or researcher MSB 4806 (AMF)
Zeiss LSM 510 META/FCS confocal microscope, inverted, with 3 channels and a programmable detector core or researcher West Complex, center for cell signalling (AMF)
Zeiss LSM 700 confocal microscope, inverted, with 4 channels, ZEN software core or researcher MSB 4806 (AMF)
Zeiss LSM 710 Multiphoton confocal. upright, 4 channels, 6 laser lines, Coherent Chameleon IR laser, core or researcher MSB 4809 (AMF)
Zeiss LSM 880 Reads 10 dyes simultaneously, high sensitivity detector, environmental chamber for live cell imaging core or researcher MSB 4809 (AMF)
Leica Ultracut UC7 ultramicrotome preparation of samples for transmission electron microscopy core or researcher MSB 4806 (AMF)
Olympus BX51 with digital camera Brightfield, Fluorescence, Phase Contrast and DIC core or researcher MSB 4806 (AMF)
Olympus SZX12 dual objective stereo microscope with digital camera Brightfield, Darkfield and Fluorescence core or researcher MSB 4806 (AMF)
High Resolution Vacuum Evaporator for rotary shadowing metal coating core or researcher MSB 4806 (AMF)
Leica Ultracut Ultramicrotomes (3) core or researcher MSB 4806 (AMF)
Critical Point Dryer core or researcher MSB 4806 (AMF)
Sputter Coater core or researcher MSB 4806 (AMF)
Computing clusters, dedicated access, 8 cores, 128GB RAM Bioinformatics
Computational infrastructure that is essential for analyzing high-throughput biological data, including shared access to an HPC cluster with >5,000 computing cores, >30,000 GB RAM, and >1 PB disk space, and dedicated access to several high-memory multicore development servers Bioinformatics
Bioconductor software for bioinformatics and statistical computing, over 600 packages Bioinformatics
Bruker Avance III NMR spectrometer 800MHz core or researcher Chem 133 (BioNMR)
Bruker Avance III NMR spectrometer 600MHz core or researcher Chem 133 (BioNMR)
Bruker Avance DRX NMR spectrometer 600MHz core or researcher Chem 106 (BioNMR)
Varian NMRS spectrometer 600MHz core or researcher Chem 111 (BioNMR)
Varian Inova 500 NMR spectrometer 500MHz core or researcher Chem 175 (BioNMR)
Leica ASLMD laser microdissection apparatus micro scale dissection of fixed or frozen tissue researcher MR6 G712 (BTRF)
Arcturus (Life Technologies) XT-TI laser microdissection apparatus micro scale dissection of fixed or frozen tissue under bright-field, phase contrast or fluorescence visualization researcher MR6 G712 (BTRF)
Pathology Devices TMArrayer semi-automated tissue microarray apparatus production of tissue microarrays core MR6 G712 (BTRF)
QIAGEN QIAcube robotic workstation fully automated protein and nucleic acid extraction core MR6 G712 (BTRF)
Next Advance Bullet Blender tissue homogenizer tissue disruption/homogenization for fresh, frozen or fixed tissue core MR6 G712 (BTRF)
Miltenyi Biotech gentleMACS™ Dissociator fresh tissue dissociation for viable cells core MR6 G712 (BTRF)
Bio-Rad iMark Absorbance Microplate Reader 96-well format plate reader; available filters 415,450,595,620,655,750 nm core or researcher MR6 G712 (BTRF)
Bio-Rad Experion microcapillary electrophoresis system microscale/LabChip RNA and DNA analysis core MR6 G712 (BTRF)
NanoDrop spectrophotometer microscale DNA and RNA quantitation core or researcher MR6 G712 (BTRF)
DAKO Autostainer/Autostainer Plus automated immunohistochemistry platform core MR6 G712 (BTRF)
DAKO PT Link pretreatment module semi-automated depariffinization and antigen retrieval for immunohistochemistry core MR6 G712 (BTRF)
Leica 3050S research cryostat preparation of frozen tissue sections core or researcher MR6 G710 (BTRF)
Olympus BX41TF with 12 megapixel Digital Camera and Olympus Microsuite Pathology bright-field photomicroscopy and remote collaboration core or researcher MR6 G712 (BTRF)
Nikon COOLSCOPE bright-field photomicroscopy core or researcher MR6 G712 (BTRF)
Leica SCN400 digital whole-slide scanner with SlidePath Digital Image Hub, Tissue IA, and OpTMA Module bright-field whole slide imaging, image analysis, and tissue microarray de-arraying, annotation, and analysis core MR6 B703 (BTRF)
Sakura Finetek VIP5 Processor automated processing of formalin-fixed tissue core MR6 G710 (BTRF)
Sakura Finetek Tissue-Tek TEC preparation of paraffin blocks core MR6 G710 (BTRF)
Microm HM 325 with collimator microtome for sectioning paraffin blocks and tissue microarrays core MR6 G710 (BTRF)
MVE-Chart 1536P vapor phase LN2 freezers vapor phase biospecimen storage core MR6 B501 (BTRF)
-30º C freezer biospecimen storage core MR6 G701A (BTRF)
-80º C freezers biospecimen storage core MR6 G701A (BTRF)
Illumina MiSeq sequencing system small, rapid next gen sequencing projects core Pinn 1044 (DNA)
Illumina NextSeq sequencing system small genome sequencing, exome sequencing, transcriptome sequencing core Pinn 1044 (DNA)
Applied Biosystems Prism QuantStudio 6 Flex Real Time PCR System real time PCR core Pinn 1076 (DNA)
Agilent Bioanalyzer capillary electrophoresis of small samples of DNA and RNA for quality control core Pinn 1044 (DNA)
NanoVue Spectrophotometer measuring absorbances of small volumes, especially quantitation of DNA core Pinn 1076 (DNA)
QIAcube DNA/RNA purification apparatus nucleic acid extraction core Pinn 1044 (DNA)
Bio-Rad QX200™ Droplet Digital™ PCR System absolute quantitation of DNA and RNA core or researcher Pinn 1076 (DNA)
Sage Pippin Prep preparation of libraries for NGS using agaroe gel electrophoresis to isolate the desired size(s) core Pinn 1076 (DNA)
Covaris S2 Shear DNA to sizes suitable for sequencing core or researcher Pinn 1076 (DNA)
Agilent Tape Station Analysis of RNA by electrophoretic separation core Pinn 1076 (DNA)
SensorMedics Vmax Metabolic Stress Testing Systems Clinical measurement of oxygen consumption during exercise core Barringer 2406 (EPCL)
BOD POD GS Body Composition tracking system (COSMED) Air displacement plethysmography system for determining body composition, e.g. fat free tissue and fat tissue core Barringer 2406 (EPCL)
GE Case Exercise Testing System including ECG and Treadmill Clinical measurement of oxygen consumption during exercise core Barringer 2406 (EPCL)
Monark Arm Crank Ergometer Upper body ergometer core Barringer 2406 (EPCL)
SensorMedics Deltatrac Bedside Metabolic System Measurement of basal or resting metabolism core Barringer 2406 (EPCL)
Quinton Q-Stress ECG system Electrocardiograph for exercise testing; compatible with Lode ergometer core Barringer 2406 (EPCL)
Lode Bicycle Ergometer Electronically calibrated research ergometer core Barringer 2406 (EPCL)
sliceOmatic (TomoVision) software Medical image analysis software for measurement, segmentation and analyzes of multi-slice scanner data. core Barringer 2406 (EPCL)
Brachial Analyzer (MIA Vascular Research Tools 5) software Medical image analysis software for the assessment of FMD/endothelial function of brachial artery from ultrasound image data. core Barringer 2406 (EPCL)
Attune™ NxT high throughput cytometry with 4 lasers, 14 colors and autosampler core Pinn 2013 (FCCF)
Becton Dickinson FACSVantage SE Turbo Sorter with DIVA cell sorter 10 color, 4 way sorting, sterile sorting, BSL2 materials, with 3 lasers core Pinn 2013 (FCCF)
Becton Dickinson Fortessa LSRII benchtop analyzer 4 lasers, 17 detectors at up to 30,000 cells/sec core or researcher Pinn 2011 (FCCF)
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer 3 laser, 5 color analyses core or researcher Pinn 2011 (FCCF)
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer 4 laser, 10 color analyses with Automated Micro-Sampler system for 96 well plates core or researcher Pinn 2011 (FCCF)
Becton Dickinson FACSCalibur Benchtop Analyzer 2 laser, 4 color analyses core or researcher Pinn 2011 (FCCF)
Becton Dickinson FACSCalibur Benchtop Analyzer 2 laser,  color analyses researcher Pinn 2011 (FCCF)
Beckman Coulter CyAn ADP LX 9 Color 3 laser, 9 color analysis at up to 30,000 cells/sec core or researcher Pinn 2011 (FCCF)
Amnis ImagestreamX Mark II imaging cytometer, for dark field, bright field and fluorescent images with 4 lasers, 12 channels, 20X, 40X, 60X magnifications, 96 well plate automation core or researcher Pinn 2011 (FCCF)
Luminex MAGPIX® measures multiple analytes such as chemokines from mouse or human sample Pinn 2011 (FCCF)
BD Influx cell sorter for BSL2 samples in custom Baker biosafety cabinet 6 way sorting of BSL2 samples using 5 lasers, 15 colors core Pinn 2013 (FCCF)
FACSAria™ Fusion sorting of BSL2 samples with 5 lasers, 15 colors, 4 way sorting (FCCF)
Fluidigm CyTOF 2 Helios analysis of multiple parameters at single cell level core Pinn 2013 (FCCF)
AutoMACS Pro Isolation of cell populations labelled with 50 nm superparamagnetic particles conjugated to antibodies to cell surface antigens researcher Pinn 2011 (FCCF)
Data analysis work station (5) 4 Mac stations and 1 PC with FloJo and Modfit software researcher Pinn 2011 (FCCF)
Leica DM IRB inverted microscopes core MR5 G339 (GEMM)
Leitz micromanipulators core MR5 G339 (GEMM)
Sutter P-87 micropipette puller core MR5 G339 (GEMM)
De Fonbrune microforge core MR5 G339 (GEMM)
Bio-Cool BCIV40A Controlled-Rate Freezer core MR5 G339 (GEMM)
XYClone laser system core MR5 G339 (GEMM)
BTX ECM630 Electroporator core Pinn 2223 (GEMM)
Eppendorf EP Gradient Mastercycler core Pinn 2223 (GEMM)
UVIdoc LCD Gel Documentation System core Pinn 2223 (GEMM)
Matrix Assisted Laser Desorption Ionization-Time Of Flight mass spectrometer (MALDI-TOF), Bruker Microflex measure mass of intact molecules, normally peptides, protein, also small pieces of DNA, other molecules core or researcher Pinn 1105 (BAF)
Thermo LTQ Velos Orbitrap mass spectrometer determination of peptide sequences for protein identification in complex mixtures of proteins, relative quantitation of proteins in mixtures, identification of phosphorylation sites and other post translational modifications core Pinn 1105 (BAF)
Thermo TSQ Quantum Access absolute quantitation of peptides and proteins with appropriate standards.

Triple quadrupole machine.

core Pinn 1105 (BAF)
FEI Tecnai™ Spirit transmission electron microscopy of negatively stained samples, cryo-electron microscopy researcher Life Sciences Annex, Snyder (MEMC)
FEI Tecnai™ F20 cryo-electron microscopy (moderate resolution), high resolution negative stain electron microscopy researcher Life Sciences Annex, Snyder (MEMC)
FEI Titan Krios high resolution cryo-electron microscopy researcher Life Sciences Annex, Snyder (MEMC)
FEI Vitrobot sample vitrification for cryo-transmission electron microscopy researcher Life Sciences Annex, Snyder (MEMC)
7 Tesla Bruker/Siemens ClinScan small animal MRI MRI scans of small animals core or researcher MR4 1184 (MIC)
Caliper IVIS Spectrum bioluminescence and fluorescence scanner fluorescence and luminescence scanner for tissue samples core or researcher Snyder (MIC)
Bruker Aibira Si  trimodal PET/SPCET/CT scanner scan small animals using positron emission, gamma emission and X-rays with sub-millimeter resolution Snyder (MIC)
Siemens Eclipse Cyclotron production of isotopes for PET scanning core and Siemens/PETNET Snyder (MIC)
Radiochemistry modules synthesis of radiolabels core
ultrasound scanner ultrasound imaging
Tissue processors for formalin and Bouin’s fixed tissues core MR5 1035 (RHC)
tissue embedding station MR5 1035 (RHC)
microtomes preparing thin sections for microscopy core MR5 1035 (RHC)
stainer, automated staining of tissue sections core MR5 1035 (RHC)
cryostat preparing frozen tissue sections for microscopy core MR5 1035 (RHC)
fluorescent plate reader assays using fluorescent reagents in 6 to 384 well plates researcher Pinn 1074 (BAF)
circular dichroism spectropolarimeter, Aviv 410 secondary structure of proteins, including melting studies researcher Pinn 1074 (BAF)
Bio-Rad FX fluorescent gel scanner scanning gels stained with Sypro Ruby and other fluorescent stains. 3 laser sources, several filters to cover common visible wavelengths. core Pinn 1051 (BAF)
Dionex HPLC system for 0.3 to 4.6 mm diameter columns analysis of small samples of peptides, proteins and other molecules core Pinn 1051 BAF)
Bio-Rad GS-800 densitometer scanning X-ray type films, gels stained with visible stains like Coomassie and silver researcher Pinn 1074 (BAF)
ImageQuant Tl software relative quantitation of bands on gels and film researcher Pinn 1074 (BAF)
Aperio ScanScope brightfield scanning of microscopy slide to digital images at magnifications of 20X, 40X, 63X for digital pathology researcher or core Pinn 1074 (BAF)
fortéBIO Octet96 molecular interactions using BioLayer Interferometry researcher Pinn 1074 (BAF)
Acea xCELLigence RTCA real time cell analysis instrument real time, continuous measurement of cellular activity, including migration, based on impedance measurements researcher Pinn 1051 (BAF)
Biotek ELx800 absorbance plate reader absorbance measurements on microtiter plates at 450, 562, 600, 660 nm researcher Pinn 1074 (BAF)
Inverted microscopes, Axio and Olympus core or researcher Pinn 2034 (TCF)
Olympus IM microscope core or researcher Pinn 2034 (TCF)
CO2 incubators core or researcher Pinn 2034 (TCF)
Incubators maintenance of insect cell lines core Pinn 2034 (TCF)
cold storage, -80°C, liquid nitrogen cell storage core Pinn 2034 (TCF)
Biosafety cabinets (3) cell culture Pinn 2034 (TCF)

*Location information is building name and room number. MSB, Medical School Building. Pinn Hall was formerly called Jordan Hall

Core abbreviations: AMF, Advanced Microscopy Facility; BAF, Biomolecular Analysis Facility; BioNMR, Biomolecular Magnetic Resonance Facility; BTRF, Biorepository and Tissue Research Facility; DNA, DNA Sciences Core; EPCL, Exercise Physiology Core Laboratory; FCCF, Flow Cytometry Core Facility; GEMM, Genetically Engineered Murine Model Core; MEMC, Molecular Electron Microscopy Core; MIC, Molecular Imaging Core; RHC, Research Histology Core; TCF, Tissue Culture Facility.