Equipment in research cores

Major equipment in the School of Medicine research cores

Equipment	Use	Operated by	Location*	Core
Operetta High-Content Imaging System	It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images	Core or Researcher	OMS 4806	Advanced Microscopy Facility
LSM 710		Core or Researcher	OMS 4806	Advanced Microscopy Facility
Zeiss Sigma VP HD FE-SEM		Core or Researcher	OMS 4806	Advanced Microscopy Facility
Zeiss LSM700 confocal microscope		Core or Researcher	OMS 4806	Advanced Microscopy Facility
LSM 880 Confocal with Airyscan	Reads 10 dyes simultaneously, high sensitivity detector, environmental chamber for live cell imaging	Core or Researcher	OMS 4806	Advanced Microscopy Facility
JOEL JEM-1230 TEM		Core or Researcher	OMS 4806	Advanced Microscopy Facility
Olympus BX51	Brightfield, Fluorescence, Phase Contrast and DIC	Core or Researcher	OMS 4806	Advanced Microscopy Facility
Leica Thunder with TIRF		Core or Researcher	OMS 4806	Advanced Microscopy Facility
Olympus SZX12 dual objective stereo microscope with digital camera	Brightfield, Darkfield and Fluorescence	Core or Researcher	OMS 4806	Advanced Microscopy Facility
High Resolution Vacuum Evaporator for rotary shadowing	metal coating	Core or Researcher	OMS 4806	Advanced Microscopy Facility
Leica Ultracut Ultramicrotomes (3)		Core or Researcher	OMS 4806	Advanced Microscopy Facility
Critical Point Dryer		Core or Researcher	OMS 4806	Advanced Microscopy Facility
Sputter Coater		Core or Researcher	OMS 4806	Advanced Microscopy Facility
Thermo Orbitrap Exploris 480	Complex mixture and PTM analysis as well as relative quantification	Researcher	Pinn 1051	Biomolecular Analysis Facility
Circular Dichroism Spectropolarimeter, JASCO J-1500	Secondary structure of proteins, including melting studies	Researcher	Pinn 1074	Biomolecular Analysis Facility
Thermo Orbitrap ID-X	Scanning X-ray type films, gels stained with visible stains like Coomassie and silver	Researcher	Pinn 1074	Biomolecular Analysis Facility
fortéBIO Octet96	molecular interactions using BioLayer Interferometry	Researcher	Pinn 1074	Biomolecular Analysis Facility
Computing clusters, dedicated access, 32 cores, 252GB RAM	Computational infrastructure that is essential for analyzing high-throughput biological data	Bioinformatics/maintained by UVA ITS	University Data Center OLD IVY	Bioinformatics
Computing clusters, dedicated access, 55 cores, 378GB RAM	Computational infrastructure that is essential for analyzing high-throughput biological data	Bioinformatics/maintained by UVA ITS	University Data Center OLD IVY	Bioinformatics
Computing clusters, dedicated access, 72 cores, 378GB RAM	Computational infrastructure that is essential for analyzing high-throughput biological data	Bioinformatics/maintained by UVA ITS	University Data Center OLD IVY	Bioinformatics
Bruker Avance III NMR spectrometer 800MHz with HCN Zpfg cryoprobe	Performs wide range of solution NMR experiments	Core or Researcher	Chem 138	BioNMR
Bruker Avance III NMR spectrometer 600MHz with HCN Zpfg probe	Performs wide range of solution NMR experiments	Core or Researcher	Chem 138	BioNMR
Varian NMRS 600 spectrometer with multiple probes	Performs wide range of solution NMR experiments	Core or Researcher	Chem 122	BioNMR
Varian NMRS 600 spectrometer with multiple probes	Performs wide range of solution NMR experiments	Core or Researcher	Chem 175	BioNMR
Varian Inova 500 NMR spectrometer with multiple probes	Performs wide range of solution NMR experiments	Core or Researcher	Chem 175	BioNMR
Arcturus (Life Technologies) XT-TI laser microdissection apparatus	micro scale dissection of fixed or frozen tissue under bright-field, phase contrast or fluorescence visualization	researcher	MR6 B703	Biorepository & Tissue Research Facility
Pathology Devices TMArrayer semi-automated tissue microarray apparatus	Semi-automated construction of tissue microarrays	Core	MR6 G714	Biorepository & Tissue Research Facility
QIAGEN QIAcube robotic workstation	fully automated protein and nucleic acid extraction	Core	MR6 G710	Biorepository & Tissue Research Facility
Next Advance Bullet Blender tissue homogenizer	tissue disruption/homogenization for fresh, frozen or fixed tissue	Core	MR6 G712	Biorepository & Tissue Research Facility
Miltenyi Biotech gentleMACS™ Dissociator	Fresh tissue dissociation for viable cells	Core	MR6 G712	Biorepository & Tissue Research Facility
Bio-Rad iMark Absorbance Microplate Reader	96-well format plate reader; available filters 415,450,595,620,655,750 nm	Core or Researcher	MR6 G712	Biorepository & Tissue Research Facility
DAKO Autostainer Plus	Automated immunohistochemistry platform	Core	MR6 G710	Biorepository & Tissue Research Facility
DAKO PT Link pretreatment module	Semi-automated depariffinization and antigen retrieval for immunohistochemistry	Core	MR6 G710	Biorepository & Tissue Research Facility
Olympus BX41TF with 12 megapixel Digital Camera and Olympus Microsuite Pathology	Bright-field photomicroscopy and remote collaboration	Core or Researcher	MR6 G712	Biorepository & Tissue Research Facility
Sakura Finetek VIP5 Processor	Automated processing of formalin-fixed tissue	Core	MR6 G714	Biorepository & Tissue Research Facility
Sakura Finetek Tissue-Tek TEC	Preparation of paraffin blocks	Core	MR6 G714	Biorepository & Tissue Research Facility
Microm HM 325 with histocollimator	Microtome for sectioning paraffin blocks and tissue microarrays	Core	MR6 G714	Biorepository & Tissue Research Facility
MVE-Chart vapor phase LN2 freezers	Cryogenic biospecimen storage	Core	MR6 B501B	Biorepository & Tissue Research Facility
-30º C freezer	biospecimen storage	Core	MR6 G701A	Biorepository & Tissue Research Facility
-80º C freezers	biospecimen storage	Core	MR6 (Multiple Locations)	Biorepository & Tissue Research Facility
QIAGEN TissueLyser II	Tissue disruption/homogenization for fresh, frozen or fixed tissue	Core	MR6 G710	Biorepository & Tissue Research Facility
Thermo CryoStarNX50	Preparation of frozen tissue sections	Core or Researcher	MR6 G714	Biorepository & Tissue Research Facility
Hamamatsu Nanozoomer S360	Digital pathology: high-throughput whole slide scanning (brightfield)	Core	MR6 G714	Biorepository & Tissue Research Facility
Pathcore Flow image management system	Digital pathology: web-based image storage, collaboration, & sharing	Core or Researcher	MR6 G714	Biorepository & Tissue Research Facility
Visiopharm ONCOTOPIX software	Digital pathology: whole slide, multi-color image analysis	Core	MR6 G714	Biorepository & Tissue Research Facility
Nexcelom Cellometer Auto 2000 Automatic Cell Viability Counter system	Fluorescence-based cell counting	Core	MR6 G712	Biorepository & Tissue Research Facility
Agilent 4150 Tapestation	Labchip-based RNA/DNA quality assessment	Core	MR6 G710	Biorepository & Tissue Research Facility
NanoDrop One Flash Spectrophotometer	Microliter volume direct read quantitation of nucleic acid solutions	Core or Researcher	MR6 G712	Biorepository & Tissue Research Facility
NanoDrop 3300 Fluorospectrometer	Microliter volume dye-based quantitation of nucleic acid solutions	Core or Researcher	MR6 G712	Biorepository & Tissue Research Facility
Ventana Discovery Ultra	Automated immunohistochemistry and chromogenic RNA in-situ platform	Core	MR6 G710	Biorepository & Tissue Research Facility
StatSpin CytoFuge 2	Creation of cytospin cytology preps	Core	MR6 G710	Biorepository & Tissue Research Facility
10X Genomics Chromium Controller	Single Cell RNA Seq, single nuclei ATAC-seq and single cell proteogenomics	Core	Pinn 1076	Genome Analysis Technology Core
Illumina MiSeq sequencing system	Small genome sequencing, targeted gene sequencing, 16S metagenomics, and library QC	Core	Pinn 1044	Genome Analysis Technology Core
Illumina NextSeq sequencing system	Broad genomics sequencing applications including RNA-Seq (bulk and single cell), whole exome sequencing, high throughput metagenomics and targeted resequencing	Core	Pinn 1044	Genome Analysis Technology Core
Applied Biosystems Prism QuantStudio 6 Flex Real Time PCR System	Real time PCR for gene expression and genotyping applications.	Core	Pinn 1076	Genome Analysis Technology Core
Agilent Bioanalyzer	Capillary electrophoresis of small samples of DNA and RNA for quality control	Core or Researcher	Pinn 1076	Genome Analysis Technology Core
NanoVue Spectrophotometer	Measuring absorbances of small volumes, especially quantitation of DNA	Core	Pinn 1076	Genome Analysis Technology Core
Qubit fluorometer	Quantitating small samples of DNA and RNA by fluorescence	Core or Researcher	Pinn 1044	Genome Analysis Technology Core
Bio-Rad QX200™ Droplet Digital™ PCR System	Absolute quantitation of DNA and RNA	Core or Researcher	Pinn 1076	Genome Analysis Technology Core
Sage Pippin Prep	Preparation of libraries for NGS using agaroe gel electrophoresis to isolate the desired size(s)	Researcher	Pinn 1076	Genome Analysis Technology Core
Covaris S2	Shear DNA to sizes suitable for sequencing	Researcher	Pinn 1076	Genome Analysis Technology Core
Agilent Tape Station	QC instrument for RNAs and DNA. It is used to determine RNA integrity number (RIN) and for sizing NGS libraries prior to sequencing	Core or Researcher	Pinn 1076	Genome Analysis Technology Core
Vyaire Vmax	Clinical measurement of oxygen consumption during exercise	Core	Collins 2406	Exercise Physiology Core Laboratory
BOD POD GS Body Composition tracking system (COSMED)	Air displacement plethysmography system for determining body composition, e.g. fat free tissue and fat tissue	Core	Collins 2406	Exercise Physiology Core Laboratory
GE Case Exercise Testing System including ECG and Treadmill	Clinical measurement of oxygen consumption during exercise	Core	Collins 2406	Exercise Physiology Core Laboratory
Monark Arm Crank Ergometer	Upper body ergometer	Core	Collins 2406	Exercise Physiology Core Laboratory
Quinton Q-Stress ECG system	Electrocardiograph for exercise testing; compatible with Lode ergometer	Core	Collins 2406	Exercise Physiology Core Laboratory
Lode Corival Bike	Electronically calibrated research ergometer	Core	Collins 2406	Exercise Physiology Core Laboratory
Brachial Analyzer (MIA Vascular Research Tools 5) software	Medical image analysis software for the assessment of FMD/endothelial function of brachial artery from ultrasound image data.	Core	Collins 2406	Exercise Physiology Core Laboratory
Attune™ NxT	high throughput cytometry with 4 lasers, 14 colors and autosampler	Core	Pinn 2013	Flow Cytometry Core
Cytek Aurora Borealis	5 laser 64 fluorescent detectors with Autosampler	Core	Pinn 2013	Flow Cytometry Core
Cytek Aurora Northern Lights	3 laser 38 fluorescent detectors with Autosampler	Core	Pinn 2013	Flow Cytometry Core
Becton Dickinson Fortessa LSRII benchtop analyzer	4 lasers, 17 detectors at up to 30,000 cells/sec	Core or researcher	Pinn 2011	Flow Cytometry Core
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer	3 laser, 5 color analyses	Core or researcher	Pinn 2011	Flow Cytometry Core
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer	4 laser, 10 color analyses with Automated Micro-Sampler system for 96 well plates	Core or researcher	Pinn 2011	Flow Cytometry Core
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer	2 laser, 4 color analyses	Core or researcher	Pinn 2011	Flow Cytometry Core
Becton Dickinson/Cytek FACSCalibur Benchtop Analyzer	2 laser, color analyses	Researcher	Pinn 2011	Flow Cytometry Core
Amnis ImagestreamX Mark II	imaging cytometer, for dark field, bright field and fluorescent images with 4 lasers, 12 channels, 20X, 40X, 60X magnifications, 96 well plate automation	Core or researcher	Pinn 2011	Flow Cytometry Core
Luminex MAGPIX®	measures multiple analytes such as chemokines from mouse or human sample		Pinn 2011	Flow Cytometry Core
BD Influx cell sorter for BSL2 samples in custom Baker biosafety cabinet	6 way sorting of BSL2 samples using 5 lasers, 15 colors	Core	Pinn 2013	Flow Cytometry Core
FACSAria™ Fusion	sorting of BSL2 samples with 5 lasers, 15 colors, 4 way sorting	Core	Pinn 2013	Flow Cytometry Core
Fluidigm CyTOF 2 Helios	analysis of multiple parameters at single cell level	Core	Pinn 2013	Flow Cytometry Core
AutoMACS Pro	Isolation of cell populations labelled with 50 nm superparamagnetic particles conjugated to antibodies to cell surface antigens	Researcher	Pinn 2011	Flow Cytometry Core
Data analysis work station (5)	4 Mac stations and 1 PC with FloJo and Modfit software	Researcher	Pinn 2011	Flow Cytometry Core
Leica DM IRB inverted microscopes		Core	OMS 1845	Genetically Engineered Murine Model Core
Leitz micromanipulators		Core	OMS 1845	Genetically Engineered Murine Model Core
Sutter P-87 micropipette puller		Core	OMS 1845	Genetically Engineered Murine Model Core
De Fonbrune microforge		Core	OMS 1845	Genetically Engineered Murine Model Core
Bio-Cool BCIV40A Controlled-Rate Freezer		Core	OMS 1845	Genetically Engineered Murine Model Core
XYClone laser system		Core	OMS 1845	Genetically Engineered Murine Model Core
NEPA21 type II super electroporator		Core	OMS 1845	Genetically Engineered Murine Model Core
Primetech PMM4G piezo micro manipulator		Core	OMS 1845	Genetically Engineered Murine Model Core
Eppendorf femtojet 4i electronic microinjector		Core	OMS 1845	Genetically Engineered Murine Model Core
BTX ECM630 Electroporator		Core	Pinn 2223	Genetically Engineered Murine Model Core
Eppendorf EP Gradient Mastercycler		Core	Pinn 2223	Genetically Engineered Murine Model Core
UVIdoc LCD Gel Documentation System		Core	Pinn 2223	Genetically Engineered Murine Model Core
FEI Tecnai™ Spirit	Transmission electron microscopy of negatively stained samples, cryo-electron microscopy	Researcher	Life Sciences Annex, Snyder	Molecular Electron Microscopy Core
FEI Tecnai™ F20	Cryo-electron microscopy (moderate resolution), high resolution negative stain electron microscopy	Researcher	Life Sciences Annex, Snyder	Molecular Electron Microscopy Core
FEI Titan Krios	High resolution cryo-electron microscopy	Researcher	Life Sciences Annex, Snyder	Molecular Electron Microscopy Core
7 Tesla Bruker/Siemens ClinScan small animal MRI	MRI scans of small animals	Core or research	MR4 1184	Molecular Imaging Core
Caliper IVIS Spectrum bioluminescence and fluorescence scanner	fluorescence and luminescence scanner for tissue samplesfluorescence and luminescence scanner for tissue samples	Core or research	Life Sciences Annex, Snyder	Molecular Imaging Core
Bruker Aibira Si trimodal PET/SPCET/CT scanner	Scan small animals using positron emission, gamma emission and X-rays with sub-millimeter resolution	Core	Life Sciences Annex, Snyder	Molecular Imaging Core
Siemens Eclipse Cyclotron	Production of isotopes for PET scanning	Core and Siemens/PETNET	Life Sciences Annex, Snyder	Molecular Imaging Core
Trasis Automated Radiosynthesizer	Produce preclinical or clinical radiopharmaceuticals on a fully automated platform	Core	Life Sciences Annex, Snyder	Radiochemistry
Elixys Flex/Chem	Capable of producing a variety of preclinical and clinical research imaging agents	Core	Life Sciences Annex, Snyder	Radiochemistry
Hidex Automated Gamma Counter	Gamma detection window of 100-4,000 kEv (almost any research isotope (F18, C11, Tc99m, 125I, Ga68, Zr89, Cu64, etc.))	Core	Life Sciences Annex, Snyder	Radiochemistry
Tissue processors for formalin and Bouin’s fixed tissues		Core	MR5 1035	Research Histology
tissue embedding station		Core	MR5 1035	Research Histology
microtomes	preparing thin sections for microscopy	Core	MR5 1035	Research Histology
stainer, automated	staining of tissue sections	Core	MR5 1035	Research Histology
cryostat	preparing frozen tissue sections for microscopy	Core	MR5 1035	Research Histology
Lonza 4D Nucleofector with X unit	Transfection of mammalian cells	Core or Researcher	Pinn Hall 1071	Stem Cell Core
Inverted microscopes, Axio and Olympus		Core or Researcher	Pinn 2034	Tissue Culture Facility
Olympus IM microscope		Core or Researcher	Pinn 2034	Tissue Culture Facility
CO2 incubators		Core or Researcher	Pinn 2034	Tissue Culture Facility
Incubators	Maintenance of insect cell lines	Core	Pinn 2034	Tissue Culture Facility
cold storage, liquid nitrogen	Cell storage	Core	Pinn 2034	Tissue Culture Facility
Biosafety cabinets (2)	Cell culture		Pinn 2034	Tissue Culture Facility

Core abbreviations: AMF, Advanced Microscopy Facility; BAF, Biomolecular Analysis Facility; BioNMR, Biomolecular Magnetic Resonance Facility; BTRF, Biorepository and Tissue Research Facility; GATC, Genome Analysis and Technology Core; EPCL, Exercise Physiology Core Laboratory; FCCF, Flow Cytometry Core Facility; GEMM, Genetically Engineered Murine Model Core; MEMC, Molecular Electron Microscopy Core; MIC, Molecular Imaging Core; RHC, Research Histology Core; TCF, SCC, Stem Cell Core; Tissue Culture Facility.*Location information is building name and room number. MSB, Medical School Building. Pinn Hall was formerly called Jordan Hall