Equipment<\/strong><\/th>| Description<\/strong><\/th> | Core<\/strong><\/th> | Operated by<\/strong><\/th>\n<\/tr>\n<\/thead>\n\n | Zeiss Lightsheet 7 (Light Sheet Fluorescence Microscope)<\/a><\/td> | advanced light sheet fluorescence microscope designed for high-resolution, three-dimensional imaging of large, live, or cleared specimens, offering gentle and fast acquisition with minimal photo damage for detailed biological studies<\/td> | Advanced Microscopy Facility<\/a> | \n \nDirector: Sijie Hao, PhD \npfa2xb@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Leica STED Super Resolution System (Super-Resolution Microscope)<\/a><\/td> | advanced fluorescence microscope that employs Stimulated Emission Depletion (STED) technology to achieve ultra-high-resolution imaging beyond the diffraction limit, enabling detailed visualization of subcellular structures and molecular interactions<\/td> | Advanced Microscopy Facility<\/a> | \n \nDirector: Sijie Hao, PhD \npfa2xb@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Zeiss LSM 880 Confocal w\/ AiryScan (Confocal Laser Scanning Microscope)<\/a><\/td> | high-resolution confocal microscope that enhances image quality and sensitivity using AiryScan technology, providing exceptional detail and contrast for advanced biological and material research<\/td> | Advanced Microscopy Facility<\/a> | \n \nDirector: Sijie Hao, PhD \npfa2xb@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Zeiss LSM 700 Confocal (Confocal Laser Scanning Microscope)<\/a><\/td> | compact and versatile confocal microscope designed for high-quality imaging and analysis, featuring efficient laser scanning and sensitive detection for detailed visualization<\/td> | Advanced Microscopy Facility<\/a> | \n \nDirector: Sijie Hao, PhD \npfa2xb@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Leica STELLARIS 8 DIVE Multiphoton Confocal (Multiphoton Laser Scanning Microscope)<\/a><\/td> | sophisticated imaging system optimized for deep in vivo exploration, utilizing multiphoton excitation for high-resolution, multi-color imaging of live tissues and dynamic processes<\/td> | Advanced Microscopy Facility<\/a> | \n \nDirector: Sijie Hao, PhD \npfa2xb@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Leica Thunder Imager 3D Cell Culture w\/ TIRF (Widefield and TIRF Microscopy)<\/a><\/td> | advanced imaging system designed for high-resolution visualization of three-dimensional cell cultures, incorporating Total Internal Reflection Fluorescence (TIRF) technology for enhanced contrast and detailed observation<\/td> | Advanced Microscopy Facility<\/a> | \n \nDirector: Sijie Hao, PhD \npfa2xb@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Zeiss Sigma VP HD SEM (Scanning Electron Microscope)<\/a><\/td> | high-definition scanning electron microscope designed for detailed surface analysis and characterization, providing exceptional resolution and imaging quality under variable pressure conditions<\/td> | Advanced Microscopy Facility<\/a> | \n \nDirector: Sijie Hao, PhD \npfa2xb@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Thermo Scientific Orbitrap Exploris 480 (Mass Spectrometer)<\/a><\/td> | high-resolution mass spectrometer offering precise and accurate mass analysis for complex biological samples, featuring advanced ion optics and fast scan speeds for comprehensive proteomics research<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Thermo Scientific Orbitrap ID-X (Mass Spectrometer)<\/a><\/td> | high-performance mass spectrometer designed for complex small molecule analysis in metabolomics and lipidomics, combining Orbitrap technology with advanced fragmentation techniques for precise identification and structural elucidation<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | JASCO J-1500 Circular Dichroism Spectropolarimeter <\/a><\/td> | sophisticated instrument used for analyzing chiral molecules, providing high-sensitivity measurements of circular dichroism to study protein secondary structures, nucleic acids, and other optically active substances<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Researcher<\/td>\n<\/tr>\n | Dionex Ultimate (HPLC)<\/a><\/td> | high-performance liquid chromatography system designed for precise and reliable separation of small molecules, peptides and small proteins<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Shimadzu 8030 MALDI-TOF (Mass Spectrometer)<\/a><\/td> | high-throughput mass spectrometer that utilizes Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight technology for rapid and accurate mass analysis of biomolecules, including proteins and peptides<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | AKTA Pure 25L (FPLC)<\/a><\/td> | flexible and intuitive fast protein liquid chromatography system designed for the purification of proteins, peptides, and other biomolecules<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | SpectraMax Id3 Plate Reader<\/a><\/td> | advanced microplate reader designed for high-throughput biochemical and cell-based assays, offering multi-mode detection capabilities including absorbance, fluorescence, and luminescence<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Researcher<\/td>\n<\/tr>\n | Thermo Speedvac<\/a><\/td> | high-efficiency vacuum concentrator designed for rapid and gentle evaporation of solvents from biological and chemical samples - <2 mL tubes<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Labconco Lyophilizer<\/a><\/td> | advanced freeze-drying system designed for the efficient dehydration of biological and chemical samples, preserving their structure and integrity for long-term storage and further analysis<\/td> | Biomolecular Analysis Facility<\/a> | \n \nDirector: Nicholas Sherman, PhD \nnes3f@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Bruker Avance III 800 (NMR Spectrometer)<\/a><\/a> | \n \nFunded by National Institutes of Health Shared Instrumentation Grant refer to How To Acknowledge Core<\/a> <\/td> high-field nuclear magnetic resonance (NMR) spectrometer that provides exceptional resolution and sensitivity for detailed structural and dynamic studies of complex molecules, has 4 RF channels and helium-cooled cryoprobe<\/td> | Biomolecular Magnetic Resonance Facility<\/a> | \n \nDirector: Jeff Ellena, PhD \njfe@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Bruker Avance III 600 (NMR Spectrometer)<\/a><\/td> | 2 RF channels, 5 mm BBO iprobe, can tune to 1H, 19F, and 31P through 109Ag, sample temperature range 150C to -130C, 60 sample auto changer<\/td> | Biomolecular Magnetic Resonance Facility<\/a> | \n \nDirector: Jeff Ellena, PhD \njfe@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Varian NMRS 600 (NMR Spectrometer)<\/a><\/td> | 3 RF channels, 5 mm XDB probe, can tune from 1H to 15N<\/td> | Biomolecular Magnetic Resonance Facility<\/a> | \n \nDirector: Jeff Ellena, PhD \njfe@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Bruker Avance Neo Nanobay 400 (NMR Spectrometer)<\/a> | \n \nFunded by National Science Foundation Major Research Instrumentation Grant, refer to How To Acknowledge Core<\/a><\/td> 2 RF channels, 5 mm BBO iprobe, can tune to 1H, 19F, and 31P through 109Ag, sample temperature range 150C to -130C, 60 sample auto changer<\/td> | Biomolecular Magnetic Resonance Facility<\/a> | \n \nDirector: Jeff Ellena, PhD \njfe@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Bruker Avance Neo Nanobay 400 (NMR Spectrometer)<\/a> | \n \nFunded by National Science Foundation Major Research Instrumentation Grant, refer to How To Acknowledge Core<\/a><\/td> 2 RF channels, 5 mm BBO iprobe, can tune to 1H, 19F, and 31P through 109Ag, 60 sample auto changer<\/td> | Biomolecular Magnetic Resonance Facility<\/a> | \n \nDirector: Jeff Ellena, PhD \njfe@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Ventana Discovery Ultra staining platform <\/a> | \n<\/td> automated system designed for high-throughput immunohistochemistry (IHC) and in situ hybridization (ISH) applications, providing precise and reproducible staining results<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Hamamatsu NanoZoomer S360 digital slide scanning system<\/a><\/td> | high-speed digital slide scanning system that offers high-resolution, whole-slide imaging for pathology and research applications<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Hamamatsu NanoZoomer S60 digital slide scanning system<\/a><\/td> | High speed digital whole slide scanning system supports both brightfield and fluorescense imaging.Stored data can be access remotely via network with a dedicate software for image storage.<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | NanoString GeoMx Digital Spatial Profiler<\/a><\/td> | advanced imaging and profiling system that combines high-resolution spatial imaging with precise molecular analysis, enabling comprehensive spatially-resolved characterization of gene and protein expression<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Biorad CFX96 thermocycle System (Real-time PCR)<\/a><\/td> | high-performance real-time PCR instrument that offers precise and reliable amplification and quantification of nucleic acids, featuring advanced thermal cycling technology and multi-channel detection<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Qiagen Qiacube system (DNA\/RNA purification)<\/a><\/td> | automated sample preparation instrument designed to streamline and enhance the efficiency of nucleic acid purification, enabling standardized and high-quality extraction of DNA, RNA, and proteins from a variety of sample types<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Thermo Scientific Nanodrop one spectrophometer <\/a><\/td> | compact and user-friendly instrument designed for quick and accurate quantification and analysis of nucleic acids, proteins, and other biomolecules, requiring minimal sample volumes<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Researcher<\/td>\n<\/tr>\n | Epredia Tissue processor <\/a><\/td> | advanced automated system designed for efficient and reliable preparation of tissue samples for histological analysis, providing precise control over processing protocols<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Thermo CryoStarNX50 research cryostats <\/a><\/td> | high-precision research cryostat designed for rapid and reliable sectioning of frozen tissue samples<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Microtome 325 <\/a><\/td> | precision instrument used for cutting thin sections of tissue samples for microscopic examination<\/td> | Biorepository and Tissue Research Facility<\/a> | \n \nDirector: Pat Pramoonjago, PhD \npp6f@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Cos Med Quark indirect calorimetry (Metabolic Analyzer)<\/a><\/td> | analyzer that uses gas exchange data to provide information re: metabolic rate and caloric needs, which can assist with weight management; measures metabolism, EKG, and heart rate during exercise testing<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Vyaire Vyntus CPX (Cardiopulmonary Exercise Testing)<\/a><\/td> | cardiopulmonary exercise testing (CPET) system designed to measure and analyze respiratory and metabolic responses, and 12 lead EKG during exercise, providing comprehensive assessment of cardiopulmonary function; also provides real-time caloric expenditure data<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Bod Pod (Body Composition Analyzer)<\/a><\/td> | body composition assessment system that uses air displacement plethysmography to measure body fat and lean body mass, providing accurate and non-invasive analysis for health, fitness, and research applications<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Lode Corival Bike (Ergometer)<\/a><\/td> | high-performance ergometer designed for accurate and reliable measurement of physical work during exercise testing and training<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Trackmaster 2100 Treadmill (Exercise Machine)<\/a><\/td> | durable and reliable exercise machine designed for clinical and research settings, offering precise control and measurement of speed and incline for accurate cardiovascular and performance testing<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Woodway 4front Treadmill (Exercise Machine)<\/a><\/td> | premium treadmill designed for both performance and comfort for low-impact exercise, advanced speed and incline controls<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | YSI Analyzer (Biochemical Analyzer)<\/a><\/td> | high-precision instrument used for measuring blood lactate at rest an during exercise <\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Lactate Pro (Lactate Analyzer)<\/a><\/td> | user-friendly lactate analyzer designed for rapid and accurate measurement of blood lactate levels<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Vyntus Walk (Cardiopulmonary Exercise Testing)<\/a><\/td> | Assessment device designed for six minute walks and other functional tests. Provides heart rate, oxygen saturations, total walking time, and distance walked<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Ergoline Ergoselect 1200 Stress echo bike (Ergometer)<\/a><\/td> | Supine ergometer specifically designed for stress echocardiography during cycling, featuring adjustable ergometry protocols, precise load control, and seamless integration with imaging systems<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Vyaire Ergoselect 600PRecumbent Bike (Ergometer)<\/a><\/td> | high-quality ergometer designed for comfortable and accurate cardiovascular and metabolic testing in subjects who may not be able to tolerate treadmill or upright cycling due to a surgical incision or other limitation<\/td> | Exercise Physiology Core Laboratory<\/a> | \n \nDirector: Lisa Farr, M.Ed \nlms5a@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Cytek Aurora Full Spectral 3-Laser (Flow Cytometer)<\/a><\/td> | 3 lasers \u2013 blue (488nm \u2013 50mW), violet (405nm \u2013 100mW) and red (640nm \u2013 80mW) | \n2 scattering channels \nup to 38 fluorescence detectors \nAutosampler \u2013 96 well plate<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Cytek Aurora Full Spectral 5-Laser ESP (Flow Cytometer)<\/a><\/td> | 5 lasers \u2013 blue (488nm \u2013 50mW), violet (405nm \u2013 100mW), yellow-green (561nm \u2013 50mW), red (640nm \u2013 80mW) and UV (355nm \u2013 20mW) | \n3 scattering channels \nup to 64 fluorescence detectors \nPanel design of >30 colors \nAutosampler \u2013 96 well plate and 40-tube rack \nEnhanced Small Particle Detection (ESP) capable of detecting nano particles (EVs) down to 100nm.<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Cytek Aurora Full Spectral 5-Laser (Flow Cytometer)<\/a><\/td> | 5 lasers \u2013 blue (488nm \u2013 50mW), violet (405nm \u2013 100mW), yellow-green (561nm \u2013 50mW), red (640nm \u2013 80mW) and UV (355nm \u2013 20mW) | \n3 scattering channels \nup to 64 fluorescence detectors \nAutosampler \u2013 96 well plate and 40-tube rack \nPanel design of >30 colors<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Life Technologies Attune CytPix (Flow Cytometer)<\/a><\/td> | 4 laser lines - blue (488nm), red (640nm), violet (405nm), and yellow-green (561nm) | \n16 parameters (FSC, SSC and 14 fluorescent parameters) \nIncorporated high-resolution, high-speed brightfield microscope capable of capturing up to 6,000 images\/second \nAutosampler - 96-well, 384-well, and deep-well plates<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Life Technologies Attune Nxt (Flow Cytometer)<\/a><\/td> | 4 laser lines \u2013 blue (488nm), red (640nm), violet (405nm), and yellow-green (561nm) | \n16 parameters (FSC, SSC and 14 fluorescent parameters) \nAutosampler \u2013 96-well, 384-well, and deep-well plates; 1.5 mL and 2 mL microcentrifuge tube rack (up to 24 per rack); Passive cooling available for 96-well U-bottom plates and microcentrifuge tube racks<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Becton Dickinson LSRFortessa (Flow Cytometer)<\/a><\/td> | 4 laser lines (488nm, 405nm, 561nm, and 640nm) | \n18 parameters; 2 scatter, 3 colors off the blue laser, 5 colors off the yellow-green laser, 3 colors off the red and 6 off the violet.<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Becton Dickinson\/Cytek FACSCalibur Benchtop Analyzer (Flow Cytometer)<\/a><\/td> | 3 lasers \u2013 blue (488nm) violet (407nm) and red (637nm) | \nA total of five channels can be selected; 3 off of the 488nm and either 2 off the 407nm or 1 off the 407nm and 1 off the 637nm.<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Luminex MAGPIX (Multiplexing Instrument)<\/a><\/td> | multiplexing instrument that uses magnetic bead-based technology for the simultaneous quantification of multiple analytes in a single sample<\/td> | Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Cytek Aurora (Cell Sorter)<\/a> | \n \nFunded by National Institutes of Health Shared Instrumentation Grant refer to How To Acknowledge Core<\/a> <\/td> Capable of detecting up to 64 fluorescent parameters and 3 scatter parameters | \n6-way sorting 1.5ml tubes \n4-way into 5 ml tubes \n96 well plate sorting \nBSC Class II biosafety cabinet for sorting BSL-2 biohazardous samples<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Becton Dickinson Influx (Cell Sorter)<\/a><\/td> | 5 laser lines \u2013 UV (355nm), violet (405nm), blue (488nm), yellow-green (561nm), and red (640nm) | \n17 parameters \u2013 forward & side scatter and 15 fluorescence detectors \nSix-way sorting into tubes or single cell sorting directly into plates or slides \nBSC Class II biosafety cabinet for sorting BSL-2 biohazardous samples<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Sony MA900 (Cell Sorter)<\/a> | \n \nFunded by National Institutes of Health Shared Instrumentation Grant refer to How To Acknowledge Core<\/a> <\/td> 4 laser lines \u2013 violet (405nm), blue (488nm), yellow-green (561nm) and red (638nm) | \n14 parameters \u2013 forward & side scatter and 12 fluorescent detectors \nTwo and four-way sorting into tubes as well as 6-, 12-, 24-, 48-, 96-, and 384-well plates. \nBSC Class A2 level II biosafety cabinet for sorting BSL-2 biohazardous samples. \nAutomated setup<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Miltenyi Biotec autoMACS Pro Separator (Cell Sorter)<\/a><\/td> | Isolation of cell populations labelled with 50nm superparamagnetic particles | \nChoice of manually labeling cells offline or having the autoMACS Pro label them for you as part of the isolation protocol. \nSeveral different automated protocols based on: \nDensity of the antigen \nFrequency of the population \nNegative or positive selection \nIsolation directly from whole blood<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Fluidigm\u00a0CyTOF XT (Mass Cytometer)<\/a><\/td> | Analyzes cells labeled with conjugated heavy metal isotopes via time of flight at the single cell level | \n135 detection channels and over 50+ isotopic metal markers with minimal signal overlap \nFlow Core CyTOF Antibody Bank allows your lab to purchase by the test <\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Amnis ImageStreamX Mark II (Imaging Flow Cytometer)<\/a><\/td> | The ImageStreamX Mk II system is a flow cytometer that combines the capabilities of conventional flow cytometry with high-resolution microscopy, captures multiple images of individual cells while simultaneously measuring their fluorescence and scatter properties | \n4 lasers: 405nm, 488nm, 560nm, and 658nm \nProduces up to 12 high-resolution images (brightfield, darkfield, and up to 10 fluorescents \nCollects up to 1,000 cells per second \n\tAutomated 96 well plate loader<\/td> Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Hyperion XTi (Whole-slide tissue Imaging Mass Cytometer)<\/a><\/td> | The Hyperion XTi uses metal-tagged antibodies instead of fluorophores to simultaneously and reliably detect over 40 protein and RNA markers without autofluorescence interference, enabling real-time visualization without multiple acquisition cycles. It supports continuous imaging for up to 72 hours at a scanning speed of approximately 1.5 hours per square millimeter and offers three imaging modes: Preview, Cell, and Tissue.<\/td> | Flow Cytometry Core Facility<\/a> | \n \nDirector: Mike Solga, MS \nmds4z@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | 10X Genomics Chromium X (Sequencing System)<\/a><\/td> | enables high-throughput, single-cell analysis for comprehensive insights into gene expression, chromatin accessibility, and immune profiling<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Illumina NextSeq 2000 (Sequencing System)<\/a><\/td> | high-throughput platform that offers accurate and rapid DNA and RNA sequencing, supporting a wide range of applications<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Illumina MiSeq (Sequencing System)<\/a><\/td> | versatile platform designed for accurate and efficient DNA and RNA sequencing, providing high-quality data for a variety of research<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Nanopore GridION (Sequencing System)<\/a><\/td> | scalable sequencing platform that utilizes nanopore technology for real-time DNA and RNA sequencing, offering flexibility and high throughput for diverse genomic and transcriptomic analyses<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Qiagen QIAcuity Digital PCR System<\/a><\/td> | advanced platform for precise quantification of nucleic acids through digital PCR technology, offering high sensitivity and accuracy<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Applied Biosystems QuantStudio 6 Flex Real Time PCR System<\/a><\/td> | versatile platform designed for sensitive and accurate quantification of nucleic acids, supporting a wide range of applications including gene expression analysis, genotyping, and pathogen detection<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Agilent Bioanalyzer and Tape Station (Electrophoresis System)<\/a><\/td> | advanced electrophoresis systems used for automated analysis of nucleic acids and proteins, providing precise sizing, quantification, and quality assessment of samples<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | NanoVue spectrophotometer<\/a><\/td> | instrument designed for rapid and accurate measurement of nucleic acids and proteins, offering high sensitivity and minimal sample requirement<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Qubit 3.0 fluorometer<\/a><\/td> | precise instrument used for quantifying nucleic acids and proteins using fluorescent dyes, providing accurate measurements with high sensitivity and minimal sample consumption<\/td> | Genome Analysis and Technology Core<\/a> | \n \nDirector: Katia Sol-Church, PhD \nks5uq@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | NEPA21 type II super electroporator<\/a><\/td> | advanced instrument used for efficient and precise delivery of nucleic acids, proteins, and other biomolecules into cells via electroporation, facilitating molecular biology research, genetic engineering, and drug delivery applications<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Primetech PMM4G piezo micromanipulator<\/a><\/td> | high-precision instrument used for fine positioning and manipulation of micro-objects, such as cells and electrodes<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Eppendorf femtojet 4i electronic microinjector<\/a><\/td> | electronic microinjector designed for precise and controlled injection of small volumes into cells and tissues<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Leica DMIRB and DMIL inverted microscopes<\/a><\/td> | advanced imaging systems designed for observing samples from below, commonly used in biological and medical research for live cell imaging, fluorescence microscopy<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Leica MZ75 high performance stereomicroscopes<\/a><\/td> | high-performance stereomicroscope is designed for detailed and magnified observation of specimens in three dimensions<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Leitz micromanipulators<\/a><\/td> | manipulating and handling delicate specimens and micro-objects with high accuracy and control<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Sutter P-87 micropipette puller<\/a><\/td> | specialized instrument used to create custom micropipettes with precise tip geometries<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | De Fonbrune microforge<\/a><\/td> | used for shaping and refining microtools, such as microelectrodes and micropipettes<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Bio-Cool BCIV40A Controlled-Rate Freezer<\/a><\/td> | specialized device used for freezing biological samples at a controlled rate, minimizing cellular damage and ensuring uniform freezing conditions<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | XYClone laser system (Microdissection)<\/a><\/td> | precision tool used in microsurgery and micromanipulation for precise cutting, ablation, and manipulation of cells and tissues at the microscopic level<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | BTX ECM630 Electroporator<\/a><\/td> | versatile instrument used for delivering nucleic acids, proteins, and other molecules into cells via electroporation<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Eppendorf EP Gradient Mastercycler (PCR Thermal Cycler)<\/a><\/td> | thermal cycler designed for PCR (Polymerase Chain Reaction) applications, featuring gradient functionality to optimize temperature across the sample block for efficient amplification of DNA and RNA sequences<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | UVIdoc LCD Gel Documentation System (UV Imaging System)<\/a><\/td> | imaging system designed for capturing and documenting images of nucleic acid and protein gels stained with fluorescent or colorimetric dyes<\/td> | Genetically Engineered Murine Model Core<\/a> | \n \nInterim Director: Daniel Grigsby, PhD \nqsj6gh@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Vitrobot (Cryo-Electron Microscopy Prep)<\/a><\/td> | automated system used for preparing cryo-electron microscopy (cryo-EM) grids by vitrifying samples rapidly and uniformly under controlled conditions, ensuring high-quality preservation<\/td> | Molecular Electron Microscopy Core<\/a> | \n \nDirector: Michael Purdy, PhD \nmpurdy@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Leica GP2 (Cryo-Electron Microscopy Prep)<\/a><\/td> | automated system used for preparing cryo-electron microscopy (cryo-EM) grids by vitrifying samples rapidly and uniformly under controlled conditions, ensuring high-quality preservation<\/td> | Molecular Electron Microscopy Core<\/a> | \n \nDirector: Michael Purdy, PhD \nmpurdy@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | 200-kV Tecnai\u2122 F20 (Transmission Electron Microscope)<\/a> | \n \nFunded by National Institutes of Health Shared Instrumentation Grant refer to How To Acknowledge Core<\/a> <\/td> provides detailed characterization of nanostructures and biological specimens at atomic and molecular levels<\/td> | Molecular Electron Microscopy Core<\/a> | \n \nDirector: Michael Purdy, PhD \nmpurdy@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | 300-kV Titan Krios\/K3 BioQuantum (Cryo-Electron Microscope)<\/a> | \n \nFunded by National Institutes of Health Shared Instrumentation Grant refer to How To Acknowledge Core<\/a> <\/td> used for high-resolution imaging of biological macromolecules and complexes, enabling detailed insights into their structure and function at near-atomic resolution<\/td> | Molecular Electron Microscopy Core<\/a> | \n \nDirector: Michael Purdy, PhD \nmpurdy@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | 200-kV Glacios\/Falcon 4 (Cryo-Electron Microscope)<\/a><\/td> | provide high-quality structural analysis of biological specimens at near-atomic resolution<\/td> | Molecular Electron Microscopy Core<\/a> | \n \nDirector: Michael Purdy, PhD \nmpurdy@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Aquilos 2 cryo-FIB-SEM (Cryo-Electron Microscopy Prep)<\/a><\/td> | system for cryogenic focused ion beam milling of biological specimens to prepare lamellae (thin sections) for TEM imaging, including integrated fluorescent light microscope, scanning electron microscope, and cryo-liftout capabilities <\/td> | Molecular Electron Microscopy Core<\/a> | \n \nDirector: Michael Purdy, PhD \nmpurdy@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | 9.4 Tesla Bruker BioScan small animal MRI<\/a> | \n \nFunded by National Institutes of Health Shared Instrumentation Grant refer to How To Acknowledge Core<\/a> <\/td> high-field magnetic resonance imaging (MRI) system designed for detailed anatomical and functional imaging of small animals<\/td> | Molecular Imaging Core<\/a> | \n \nDirector: Maurits Jansen, PhD \nvtf5vq@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Trimodel PET\/SPECT\/CT Bruker Albira Si rodent scanner<\/a> | \n \nFunded by National Institutes of Health Shared Instrumentation Grant refer to How To Acknowledge Core<\/a> <\/td> combines positron emission tomography (PET), single-photon emission computed tomography (SPECT), and computed tomography (CT) modalities, offering comprehensive and high-resolution imaging capabilities<\/td> | Molecular Imaging Core<\/a> | \n \nDirector: Maurits Jansen, PhD \nvtf5vq@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Revvity IVIS Spectrum bioluminescence\u00a0and\u00a0fluorescence scanner<\/a><\/td> | advanced imaging system used for bioluminescence and fluorescence imaging in small animal research<\/td> | Molecular Imaging Core<\/a> | \n \nDirector: Maurits Jansen, PhD \nvtf5vq@virginia.edu<\/td> Researcher<\/td>\n<\/tr>\n | LagoX bioluminescence and fluorescence scanner<\/a><\/td> | advanced imaging system used for bioluminescence and fluorescence imaging in small animal research<\/td> | Molecular Imaging Core<\/a> | \n \nDirector: Maurits Jansen, PhD \nvtf5vq@virginia.edu<\/td> Researcher<\/td>\n<\/tr>\n | SkyScan 1276 microCT scanner<\/a><\/td> | computed tomgraphy scanner for live animals with very high resolution capability<\/td> | Molecular Imaging Core<\/a> | \n \nDirector: Maurits Jansen, PhD \nvtf5vq@virginia.edu<\/td> Researcher<\/td>\n<\/tr>\n | IBA Cyclone 18\/9 Cyclotron<\/a> | \n \nFunded by National Institutes of Health Shared Instrumentation Grant refer to How To Acknowledge Core<\/a> <\/td> high-performance medical cyclotron designed for the production of radioisotopes used in PET and SPECT imaging<\/td> | Molecular Imaging Core<\/a> | \n \nDirector: Maurits Jansen, PhD \nvtf5vq@virginia.edu<\/td> Researcher<\/td>\n<\/tr>\n | Trasis All-in-One Automated Radiosynthesizer<\/a><\/td> | specialized instrument used in nuclear medicine and radiopharmaceutical research for automated synthesis of radiotracers<\/td> | Radiochemistry Core<\/a> | \n \nDirector: Shivashankar Khanapur, PhD \nkrs5xf@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Elixys Flex\/Chem (Radiosynthesizer)<\/a><\/td> | automated radiosynthesis system designed for the production of radiotracers used in molecular imaging studies<\/td> | Radiochemistry Core<\/a> | \n \nDirector: Shivashankar Khanapur, PhD \nkrs5xf@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Elixys Pure\/Form (HPLC)<\/a><\/td> | purification, and formulation of radiotracers in nuclear medicine and molecular imaging<\/td> | Radiochemistry Core<\/a> | \n \nDirector: Shivashankar Khanapur, PhD \nkrs5xf@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Hidex Automated Gamma Counter<\/a><\/td> | quantifying gamma radiation emitted by radioactive isotopes in samples<\/td> | Radiochemistry Core<\/a> | \n \nDirector: Shivashankar Khanapur, PhD \nkrs5xf@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | Eckert & Ziegler Modular Lab (Radiosynthesizer)<\/a><\/td> | automated radiochemistry synthesis system used in the production of radiopharmaceuticals for molecular imaging<\/td> | Radiochemistry Core<\/a> | \n \nDirector: Shivashankar Khanapur, PhD \nkrs5xf@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Tissue processor formalin fixed tissues<\/a><\/td> | automate the process of dehydrating, clearing, and embedding tissues in paraffin wax<\/td> | Research Histology Core<\/a> | \n \nDirector: Sheri Vanhoose \nslv4e@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Tissue embedding station<\/a><\/td> | embedding tissue specimens in paraffin wax blocks<\/td> | Research Histology Core<\/a> | \n \nDirector: Sheri Vanhoose \nslv4e@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Microtome<\/a><\/td> | slice thin sections of biological specimens<\/td> | Research Histology Core<\/a> | \n \nDirector: Sheri Vanhoose \nslv4e@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Automated Stainer<\/a><\/td> | device that streamlines and standardizes the staining process of tissue samples on microscope slides<\/td> | Research Histology Core<\/a> | \n \nDirector: Sheri Vanhoose \nslv4e@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Cryostat<\/a><\/td> | ensure the preservation of tissue morphology and structure during sectioning<\/td> | Research Histology Core<\/a> | \n \nDirector: Sheri Vanhoose \nslv4e@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Bruker NanoString nCounter (Molecular Analysis Platform \/ RNA Multiplex Analysis)<\/a><\/td> | Enables high-throughput, multiplexed measurement of gene expression and other molecular signatures using a unique hybridization-based technology<\/td> | Spatial Biology Core<\/a> | \n \nDirector: Ana Karina de Oliveira, PhD \nak4yj@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Bruker NanoString CosMx (High-Plex in Situ Multi-Omics Analysis \/ Spatial Transcriptome and Proteome Analysis \/ Single-Cell Resolution)<\/a><\/td> | This platform to provide spatial multi-omics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution. CosMx SMI enables rapid quantification and visualization of 1,000 or 6,000 RNA, and 64 proteins.<\/td> | Spatial Biology Core<\/a> | \n \nDirector: Ana Karina de Oliveira, PhD \nak4yj@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Cytiva Via Extractor (Tissue Dissociation)<\/a><\/td> | The VIA Extractor\u2122 tissue disaggregator is a semi-automated, gentle processing that optimizes cell viability and preserves the cell content relative to the parent sample. It is a system for consistent, rapid disaggregation of solid tissue, including tumors, into viable single cells for use in high-throughput omics, flow cytometry, and culture of isolated primary cells.<\/td> | Spatial Biology Core<\/a> | \n \nDirector: Ana Karina de Oliveira, PhD \nak4yj@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | 10x Genomics CytAssist - Visium (Spatial Transcriptome Analysis in Single Cell Scale)<\/a><\/td> | Perform whole transcriptome gene expression analysis at single-cell scale with continuous tissue coverage. | \nVisium HD Spatial Gene Expression slides contain two 6.5 x 6.5 mm Capture Areas with a continuous lawn of oligonucleotides arrayed in millions of 2 x 2 \u00b5m barcoded squares without gaps, achieving single cell\u2013scale spatial resolution. The data is output at 2 \u00b5m, as well as multiple bin sizes. <\/td> Spatial Biology Core<\/a> | \n \nDirector: Ana Karina de Oliveira, PhD \nak4yj@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Bruker Isoplexis\u200b (Single-Cell Secretome Analysis \/ Cytokine Multiplex Analysis)<\/a><\/td> | This Single-Cell Functional Proteomics technology reveals the rare subset of highly polyfunctional cells that simultaneously secrete multiple cytokines. We are able to monitor more than 30 cytokines per cell using different panels for mouse and human samples.<\/td> | Spatial Biology Core<\/a> | \n \nDirector: Ana Karina de Oliveira, PhD \nak4yj@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Biosys BioReader 7000 ELISpot Plate Reader<\/a><\/td> | to evaluate enzymatic Elispot assays, Plaque\/FOCI assays or other assays<\/td> | Molecular Immunologic & Translational Sciences Core<\/a> | \n \nDirector: Ileana Mauldin, PhD \nis3v@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Guava EasyCyte Plus Benchtop Flow Cytometer<\/a><\/td> | efficient and sensitive quantitative analysis of cells using fluorescence and light scattering measurements<\/td> | Molecular Immunologic & Translational Sciences Core<\/a> | \n \nDirector: Ileana Mauldin, PhD \nis3v@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Vectra3 (Imaging System)<\/a><\/td> | high-throughput and high-resolution analysis of tissue samples, enabling detailed quantification and spatial characterization<\/td> | Molecular Immunologic & Translational Sciences Core<\/a> | \n \nDirector: Ileana Mauldin, PhD \nis3v@virginia.edu<\/td> Core<\/td>\n<\/tr>\n | Halo Software (Image Analysis Platform)<\/a><\/td> | quantitative analysis of digital pathology images<\/td> | Molecular Immunologic & Translational Sciences Core<\/a> | \n \nDirector: Ileana Mauldin, PhD \nis3v@virginia.edu<\/td> Core & Researcher<\/td>\n<\/tr>\n | CellRaft AIR system (Imaging and Recovery System)<\/a><\/td> | combines automated imaging with microfluidic technology to isolate, culture, and analyze individual cells in a controlled manner<\/td> | Genome Engineering Shared Resource<\/a> | \n \nTechnical Director: Brian Ruis, MS \nSZZ4SH@uvahealth.org<\/td> Core & Researcher<\/td>\n<\/tr>\n | Neon NXT electroporator<\/a><\/td> | advanced instrument designed for efficient delivery of nucleic acids, such as DNA and RNA, into various cell types via electroporation<\/td> | Genome Engineering Shared Resource<\/a> | \n \nTechnical Director: Brian Ruis, MS \nSZZ4SH@uvahealth.org<\/td> Core & Researcher<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1988,"featured_media":0,"parent":0,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"tags":[],"class_list":["post-3271","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\n | |
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