Aaron Mackey, PhD of the CPHG and his colleagues published Sequencing of mRNA identifies re-expression of fetal splice variants in cardiac hypertrophy in the May 17th online edtion of the Journal of Molecular and Cellular Cardiology. This study hypothesized that patterns of alternative splicing occurring during heart development recur during cardiac hypertrophy. The researchers used high-throughput sequencing of mRNA isolated from fetal rats, adult sham-operated rats, and adult rats subjected to 10 days of pressure overload. A noteworthy degree of overlap between changes in both gene and isoform expression in TAC and fetal hearts relative to sham was found. The resulting data supports the concept that mRNA splicing patterns typically associated with heart development recur as part of the hypertrophic response to pressure overload.
June 25, 2013 by