|A Sage Science Pippin Prep prepares libraries of a specific size, or size range between 100 bp – 1.5 kb. When the size(s) of interest are eluting from the end of the gel, an electrode in a collection chamber directs the DNA to the collection chamber. Run time is less than 2 hours. The size of fragments is estimated from fluorescently labeled standards.
||The standard gel used here for the Pippin Prep is 2% agarose, dye free, with external marker in a cassette that can accommodate 4 samples. Other cassettes are available from the manufacturer.
||The Covaris instrument shears nucleic acids into suitably sized pieces for Next Generation Sequencing using focused acoustic energy. Commonly a sweep of acoustic frequencies is applied to the sample. Covaris has protocols to shear DNA to sizes of 150 to 1500 base pairs.