Next Gen Sequencing

NGS technology utilizes a massively parallel approach to quantitatively assess copy number and sequence changes in DNA or RNA samples. It has become the preferred method  for integrated  approaches that simultaneously measure transcript copy numbers and sequence- based alterations. Two technologies are currently available in the core that will best fit your project, namely Illumina NextSeq™ 500 and MiSeq Sequencing System.

Each instrument supports sequencing kits that offer a range of possible cycle numbers, for instance a 150 cycle kit or 300 cycle kit.  The number of cycles in the kit  indicates how many nucleotides can be incorporated in the growing chain during sequencing.  A 150 cycle kit, for instance, can generate a 150 nt-long single end read, or  2×75 bp-long paired end reads.  Use the 300 cycles kit if you require pair-end reads that are 150 bp long.

Please keep this in mind as you are making your kit selection in iLab. Our staff can assist you in selecting the appropriate sequencing kit for your study.

To discuss projects, contact Katia Sol-Church at ks5uq@virginia.edu ph. 434.924.9953 or Yongde Bao yb8d@virginia.edu ph. 434.924.2553.