Real Time PCR

The core hasĀ  an ABI QuantStudio 6 Flex real time PCR system .

Applications

  • quantitation of gene expression
  • absolute quantitation of miRNA
  • mutation and rare sequence detection
  • allelic discrimination
  • single nucleotide polymorphisms (SNP) characterization and screening
  • microRNA quantitation
  • Next Generation Sequencing-digital PCR quantitates samples to increase sequencing accuracy and reduce repeats and enables validation of sequencing results

For more information about RT-PCR, contact Yongde Bao (434.924.2553, yb8d@virginia.edu)