{"id":1618,"date":"2022-01-24T21:39:05","date_gmt":"2022-01-25T02:39:05","guid":{"rendered":"https:\/\/med.virginia.edu\/ebrahim-lab\/?page_id=1618"},"modified":"2022-01-28T11:03:33","modified_gmt":"2022-01-28T16:03:33","slug":"high-resolution-imaging-in-live-tissues","status":"publish","type":"page","link":"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/","title":{"rendered":"High-resolution imaging in live tissues- some examples"},"content":{"rendered":"<p>Salivary gland of a live, anesthetized mouse expressing LifeAct-GFP as a marker for F-actin. Secretory vesicles become coated with F-actin as they fuse to the apical plasma membrane during exocytosis. A macrophage is also seen doing its job!<br \/>\n<div style=\"width: 1920px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-1618-1\" width=\"1920\" height=\"1080\" loop preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media1.mp4?_=1\" \/><a href=\"https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media1.mp4\">https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media1.mp4<\/a><\/video><\/div><\/p>\n<p>3D volume showing actin and non-muscle myosin II coat secretory vesicles and the apical plasma membrane in secretory cells of the salivary gland.<br \/>\n<div style=\"width: 1920px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1618-2\" width=\"1920\" height=\"1080\" loop preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media2.mp4?_=2\" \/><a href=\"https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media2.mp4\">https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media2.mp4<\/a><\/video><\/div><\/p>\n<p>Non-muscle myosin II forms a a striking polyhedral lattice (resembling a soccer ball!) on vesicle membranes during exocytosis<br \/>\n<div style=\"width: 1208px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1618-3\" width=\"1208\" height=\"1112\" loop preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media3-1.mp4?_=3\" \/><a href=\"https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media3-1.mp4\">https:\/\/med.virginia.edu\/ebrahim-lab\/wp-content\/uploads\/sites\/426\/2022\/01\/media3-1.mp4<\/a><\/video><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Salivary gland of a live, anesthetized mouse expressing LifeAct-GFP as a marker for F-actin. Secretory vesicles become coated with F-actin as they fuse to the apical plasma membrane during exocytosis. A macrophage is also seen doing its job! 3D volume showing actin and non-muscle myosin II coat secretory vesicles and the apical plasma membrane in [&hellip;]<\/p>\n","protected":false},"author":1736,"featured_media":0,"parent":0,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"tags":[],"class_list":["post-1618","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.1.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>High-resolution imaging in live tissues- some examples - Ebrahim Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"High-resolution imaging in live tissues- some examples - Ebrahim Lab\" \/>\n<meta property=\"og:description\" content=\"Salivary gland of a live, anesthetized mouse expressing LifeAct-GFP as a marker for F-actin. Secretory vesicles become coated with F-actin as they fuse to the apical plasma membrane during exocytosis. A macrophage is also seen doing its job! 3D volume showing actin and non-muscle myosin II coat secretory vesicles and the apical plasma membrane in [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/\" \/>\n<meta property=\"og:site_name\" content=\"Ebrahim Lab\" \/>\n<meta property=\"article:modified_time\" content=\"2022-01-28T16:03:33+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/\",\"url\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/\",\"name\":\"High-resolution imaging in live tissues- some examples - Ebrahim Lab\",\"isPartOf\":{\"@id\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/#website\"},\"datePublished\":\"2022-01-25T02:39:05+00:00\",\"dateModified\":\"2022-01-28T16:03:33+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"High-resolution imaging in live tissues- some examples\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/#website\",\"url\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/\",\"name\":\"Ebrahim Lab\",\"description\":\"University of Virginia School of Medicine\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/med.virginia.edu\/ebrahim-lab\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"High-resolution imaging in live tissues- some examples - Ebrahim Lab","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/med.virginia.edu\/ebrahim-lab\/high-resolution-imaging-in-live-tissues\/","og_locale":"en_US","og_type":"article","og_title":"High-resolution imaging in live tissues- some examples - Ebrahim Lab","og_description":"Salivary gland of a live, anesthetized mouse expressing LifeAct-GFP as a marker for F-actin. 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