James P. Landers

Professor, Chemistry

Primary Location

PO Box 400319

Primary Phone Number

434.243.8658

Bio & Overview

Almost every aspect of the biochemical, biomedical and clinical sciences involves separation of species in complex matrices. Electrophoresis has been a benchmark technique for separation and characterization of biologically-active species. Instead of using conventional slab gel electrophoretic approaches, electrophoresis in micron-scale capillaries using applied fields as high as 30,000 volts, results in unprecedented resolution with unique selectivities and short analysis times. As a result of the microscalar nature of the capillary, only microliters of reagent are consumed by analysis with only a few nanoliters of samples injected for analysis. These characteristics, as well as the ability for on-line detection with laser-induced fluorescence sensitivities in the attomole (10-18 moles) range, made capillary electrophoresis (CE) appealing as a replacement for electrophoretic gels in the biomedical and clinical arenas. We have demonstrated the potential impact of CE on clinical diagnostics through the development of new CE-based assays for measuring kidney function, detecting multiple sclerosis and viral infections, screening for lymphoma, as well as for diagnosing drug abuse and alcoholism. While the diagnostic impact of standard CE technology is clear, an alternative platform for electrophoresis in microscalar structures has evolved in the form of microchip electrophoresis. The use of microfabricated glass devices containing etched capillary-like channels provides an electrophoretic platform akin to CE but with more flexibility. "Microchip electrophoresis" allows for analysis times to be decreased by an order of magnitude over times achievable by CE (as fast as 10-200 seconds) and two orders of magnitude faster than gel electrophoresis. This provides obvious value to clinical diagnostic laboratories in terms of more rapid turn around time and capability for high throughput screening. We have demonstrated this with the detection T-cell and B-cell lymphoma in a separation remarkably faster than with conventional means. With a program focused on the application of miniaturized electrophoretic technology to the clinical and forensic sciences, our current efforts involve broadening the scope of applications for microchip technology. This involves addressing issues associated with integrating functions other than 'separation' onto microchips. For example, we are focused on defining approaches for integrating DNA sample preparation into microchips. PCR amplification of DNA carried out using infrared-mediated thermocycling for rapid on-chip amplification and rapid DNA extraction using microchamber-bound solid phases are two examples of our integration efforts. The successful integration of DNA extraction and amplification will lead to the development of an "Integrated Diagnostic" or ID-chip, which we ultimately hope will improve laboratory medicine. Efforts are also underway to 1) define better detection systems using acouto-optic technology, 2) develop multichannel devices for high-throughput analysis using this optical technology, 3) explore proteomic aspects of disease using multi-dimensional microchips for protein separations, and 4) apply the relevant methods to forensic applications.

Academic Information

Department: Chemistry

Academic Title: Professor

Primary Education

BS, Biochemistry, University of Guelph, 1983
PhD, Biochemistry, University of Guelph, 1988

Other Appointments

Molecular Physiology and Biological Physics
Mechanical and Aerospace Engineering

Other Affiliations

Member, Chemical and Structural Biology Program
BIMS

Research Interests

Bioanalytical Chemistry on Microchips

Research Disciplines

Biophysics
Biophysics & Structural Biology
Biotechnology
Chemistry
Mechanical and Aerospace Engineering

Research Opportunities

Biophysics & Structural Biology
Biotechnology

Selected Publications

2022

Turiello, R., Nouwairi, R. L., & Landers, J. P. (2023). Taking the microfluidic approach to nucleic acid analysis in forensics: Review and perspectives. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 63. doi:10.1016/j.fsigen.2022.102824

Dignan, L. M., Karas, S. M., Mighell, I. K., Treene, W. R., Landers, J. P., & Woolf, S. (2022). A novel method for inward fluid displacement in centrifugal microdevices for highly integrated nucleic acid processing with long-term reagent storage. ANALYTICA CHIMICA ACTA, 1221. doi:10.1016/j.aca.2022.340063

2021

Dignan, L. M., Turiello, R., Layne, T. R., O'Connell, K. C., Hickey, J., Chapman, J., . . . Landers, J. P. (2021). An ultrafast SARS-CoV-2 virus enrichment and extraction method compatible with multiple modalities for RNA detection. ANALYTICA CHIMICA ACTA, 1180. doi:10.1016/j.aca.2021.338846

2020

Woolf, M. S., Dignan, L. M., Lewis, H. M., Tomley, C. J., Nauman, A. Q., & Landers, J. P. (2020). Optically-controlled closable microvalves for polymeric centrifugal microfluidic devices. LAB ON A CHIP, 20(8), 1426-1440. doi:10.1039/c9lc01187k

2019

Jackson, K. R., Layne, T., Dent, D. A., Tsuei, A., Li, J., Haverstick, D. M., & Landers, J. P. (2020). A novel loop-mediated isothermal amplification method for identification of four body fluids with smartphone detection. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 45. doi:10.1016/j.fsigen.2019.102195

Scott, A., Mills, D., Birch, C., Panesar, S., Li, J., Nelson, D., . . . Landers, J. P. (2019). Automated microchannel alignment using innate opto-signature for microchip electrophoresis. LAB ON A CHIP, 19(22), 3834-3843. doi:10.1039/c9lc00716d

Clark, C. P., Farmehini, V., Spiers, L., Woolf, M. S., Swami, N. S., & Landers, J. P. (2019). Real Time Electronic Feedback for Improved Acoustic Trapping of Micron-Scale Particles. MICROMACHINES, 10(7). doi:10.3390/mi10070489

Clark, C. P., Xu, K., Scatt, O., Hickey, J., Tsuei, A. -C., Jackson, K., & Landers, J. P. (2019). Acoustic trapping of sperm cells from mock sexual assault samples. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 41, 42-49. doi:10.1016/j.fsigen.2019.03.012

Dai, J., Xing, Y., Xiao, L., Li, J., Cao, R., He, Y., . . . Li, X. (2019). Microfluidic Disc-on-a-Chip Device for Mouse Intervertebral Disc-Pitching a Next-Generation Research Platform To Study Disc Degeneration. ACS BIOMATERIALS SCIENCE & ENGINEERING, 5(4), 2041-2051. doi:10.1021/acsbiomaterials.8b01522

Xu, K., Clark, C. P., Poe, B. L., Lounsbury, J. A., Nilsson, J., Lauren, T., & Landers, J. P. (2019). Isolation of a Low Number of Sperm Cells from Female DNA in a Glass-PDMS-Glass Microchip via Bead-Assisted Acoustic Differential Extraction. ANALYTICAL CHEMISTRY, 91(3), 2186-2191. doi:10.1021/acs.analchem.8b04752

Krauss, S. T., Woolf, M. S., Hadley, K. C., Collins, N. M., Nauman, A. Q., & Landers, J. P. (2019). Centrifugal microfluidic devices using low-volume reagent storage and inward fluid displacement for presumptive drug detection. SENSORS AND ACTUATORS B-CHEMICAL, 284, 704-710. doi:10.1016/j.snb.2018.12.113

2017

Nelson, D. A., Hughes, J. D., Engel, C. E., Haverstick, D. M., & Landers, J. P. (2017). Use of Dual-Force Aggregation as a Multiplexed, Rapid Point-of-Care Screening Method for White Blood Cell Counting from Whole Blood Samples. JOURNAL OF APPLIED LABORATORY MEDICINE, 2(1), 92-97. doi:10.1373/jalm.2016.022475

DuVall, J. A., Le Roux, D., Thompson, B. L., Birch, C., Nelson, D. A., Li, J., . . . Landers, J. P. (2017). Rapid multiplex DNA amplification on an inexpensive microdevice for human identification via short tandem repeat analysis. ANALYTICA CHIMICA ACTA, 980, 41-49. doi:10.1016/j.aca.2017.04.051

Thompson, B. L., Wyckoff, S. L., Haverstick, D. M., & Landers, J. P. (2017). Simple, Reagentless Quantification of Total Bilirubin in Blood Via Microfluidic Phototreatment and Image Analysis. ANALYTICAL CHEMISTRY, 89(5), 3228-3234. doi:10.1021/acs.analchem.7b00354

Birch, C., DuVall, J. A., Le Roux, D., Thompson, B. L., Tsuei, A. -C., Li, J., . . . Root, B. E. (2017). Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf. MICROMACHINES, 8(1). doi:10.3390/mi8010017

Birch, C., & Landers, J. P. (2017). Electrode Materials in Microfluidic Systems for the Processing and Separation of DNA: A Mini Review. MICROMACHINES, 8(3). doi:10.3390/mi8030076

Krauss, S. T., Holt, V. C., & Landers, J. P. (2017). Simple reagent storage in polyester-paper hybrid microdevices for colorimetric detection. SENSORS AND ACTUATORS B-CHEMICAL, 246, 740-747. doi:10.1016/j.snb.2017.02.018

Sloane, H. S., Carter, M. B., Cecil, A. E. C., Le Roux, D., Mills, D. L., & Landers, J. P. (2017). Warfarin genotyping with hybridization-induced aggregation on a poly(ethylene terephthalate) microdevice. ANALYST, 142(2), 366-374. doi:10.1039/c6an02325h

Krauss, S. T., Nauman, A. Q., Garner, G. T., & Landers, J. P. (2017). Color manipulation through microchip tinting for colorimetric detection using hue image analysis. LAB ON A CHIP, 17(23), 4089-4096. doi:10.1039/c7lc00796e

2016

Ouyang, Y., Li, J., Haverstick, D. M., & Landers, J. P. (2016). Rotation-Driven Microfluidic Disc for White Blood Cell Enumeration Using Magnetic Bead Aggregation. ANALYTICAL CHEMISTRY, 88(22), 11046-11054. doi:10.1021/acs.analchem.6b02903

Cox, J. O., DeCarmen, T. S., Ouyang, Y., Strachan, B., Sloane, H., Connon, C., . . . Cruz, T. D. (2016). A novel, integrated forensic microdevice on a rotation-driven platform: Buccal swab to STR product in less than 2 h. ELECTROPHORESIS, 37(23-24), 3046-3058. doi:10.1002/elps.201600307

Krauss, S. T., Remcho, T. P., Lipes, S. M., Aranda, R., Maynard, H. P. I. I. I., Shukla, N., . . . Landers, J. P. (2016). Objective Method for Presumptive Field-Testing of Illicit Drug Possession Using Centrifugal Microdevices and Smartphone Analysis. ANALYTICAL CHEMISTRY, 88(17), 8689-8697. doi:10.1021/acs.analchem.6b01982

DuVall, J. A., Cabaniss, S. T., Angotti, M. L., Moore, J. H., Abhyankar, M., Shukla, N., . . . Landers, J. P. (2016). Rapid detection of Clostridium difficile via magnetic bead aggregation in cost-effective polyester microdevices with cell phone image analysis. ANALYST, 141(19), 5637-5645. doi:10.1039/c6an00674d

Jackson, K. R., Borba, J. C., Meija, M., Mills, D. L., Haverstick, D. M., Olson, K. E., . . . Landers, J. P. (2016). DNA purification using dynamic solid-phase extraction on a rotationally-driven polyethylene-terephthalate microdevice. ANALYTICA CHIMICA ACTA, 937, 1-10. doi:10.1016/j.aca.2016.06.036

Sloane, H. S., Landers, J. P., & Kelly, K. A. (2016). Hybridization-Induced Aggregation Technology for Practical Clinical Testing KRAS Mutation Detection in Lung and Colorectal Tumors. JOURNAL OF MOLECULAR DIAGNOSTICS, 18(4), 546-553. doi:10.1016/j.jmoldx.2016.02.004

Thompson, B. L., Birch, C., Li, J., DuVall, J. A., Le Roux, D., Nelson, D. A., . . . Landers, J. P. (2016). Microfluidic enzymatic DNA extraction on a hybrid polyester-toner-PMMA device. ANALYST, 141(15), 4667-4675. doi:10.1039/c6an00209a

Thompson, B. L., Gilbert, R. J., Mejia, M., Shukla, N., Haverstick, D. M., Garner, G. T., & Landers, J. P. (2016). Hematocrit analysis through the use of an inexpensive centrifugal polyester-toner device with finger-to-chip blood loading capability. ANALYTICA CHIMICA ACTA, 924, 1-8. doi:10.1016/j.aca.2016.04.028

Xu, K., Liu, Q., Jackson, K. R., & Landers, J. P. (2016). The μSCAPE System: 3-Dimensional Profiling of Microfluidic Architectural Features Using a Flatbed Scanner. SCIENTIFIC REPORTS, 6. doi:10.1038/srep22246

Xu, K., Wang, X., Ford, R. M., & Landers, J. P. (2016). Self-Partitioned Droplet Array on Laser-Patterned Superhydrophilic Glass Surface for Wall-less Cell Arrays. ANALYTICAL CHEMISTRY, 88(5), 2652-2658. doi:10.1021/acs.analchem.5b03764

Krauss, S. T., Remcho, T. P., Monazami, E., Thompson, B. L., Reinke, P., Begley, M. R., & Landers, J. P. (2016). Inkjet printing on transparency films for reagent storage with polyester-toner microdevices. ANALYTICAL METHODS, 8(39), 7061-7068. doi:10.1039/c6ay01878e

DuVall, J. A., Le Roux, D., Tsuei, A. -C., Thompson, B. L., Birch, C., Li, J., . . . Landers, J. P. (2016). A rotationally-driven polyethylene terephthalate microdevice with integrated reagent mixing for multiplexed PCR amplification of DNA. ANALYTICAL METHODS, 8(40), 7331-7340. doi:10.1039/c6ay01984f

Thompson, B. L., Birch, C., Nelson, D. A., Li, J., DuVall, J. A., Le Roux, D., . . . Landers, J. P. (2016). A centrifugal microfluidic device with integrated gold leaf electrodes for the electrophoretic separation of DNA. LAB ON A CHIP, 16(23), 4569-4580. doi:10.1039/c6lc00953k

Ouyang, Y., Li, J., Phaneuf, C., Riehl, P. S., Forest, C., Begley, M., . . . Landers, J. P. (2016). Multilevel fluidic flow control in a rotationally-driven polyester film microdevice created using laser print, cut and laminate. LAB ON A CHIP, 16(2), 377-387. doi:10.1039/c5lc01332a

Liu, Q., Chernish, A., DuVall, J. A., Ouyang, Y., Li, J., Qian, Q., . . . Landers, J. P. (2016). The ARTμS: a novel microfluidic CD4+T-cell enumeration system for monitoring antiretroviral therapy in HIV patients. LAB ON A CHIP, 16(3), 506-514. doi:10.1039/c5lc01153a

2015

Strachan, B. C., Sloane, H. S., Houpt, E., Lee, J. C., Miranian, D. C., Li, J., . . . Landers, J. P. (2016). A simple integrated microfluidic device for the multiplexed fluorescence-free detection of Salmonella enterica. ANALYST, 141(3), 947-955. doi:10.1039/c5an01969a

Ouyang, Y., Duarte, G. R. M., Poe, B. L., Riehl, P. S., dos Santos, F. M., Martin-Didonet, C. C. G., . . . Landers, J. P. (2015). A disposable laser print-cut-laminate polyester microchip for multiplexed PCR via infra-red-mediated thermal control. ANALYTICA CHIMICA ACTA, 901, 59-67. doi:10.1016/j.aca.2015.09.042

Sloane, H. S., Kelly, K. A., & Landers, J. P. (2015). Rapid KRAS Mutation Detection via Hybridization-Induced Aggregation of Microbeads. ANALYTICAL CHEMISTRY, 87(20), 10275-10282. doi:10.1021/acs.analchem.5b01876

Thompson, B. L., Ouyang, Y., Duarte, G. R. M., Carrilho, E., Krauss, S. T., & Landers, J. P. (2015). Inexpensive, rapid prototyping of microfluidic devices using overhead transparencies and a laser print, cut and laminate fabrication method. NATURE PROTOCOLS, 10(6), 875-886. doi:10.1038/nprot.2015.051

Durney, B. C., Bachert, B. A., Sloane, H. S., Lukomski, S., Landers, J. P., & Holland, L. A. (2015). Reversible phospholipid nanogels for deoxyribonucleic acid fragment size determinations up to 1500 base pairs and integrated sample stacking. ANALYTICA CHIMICA ACTA, 880, 136-144. doi:10.1016/j.aca.2015.03.009

Strachan, B. C., Sloane, H. S., Lee, J. C., Leslie, D. C., & Landers, J. P. (2015). Investigation of the DNA target design parameters for effective hybridization-induced aggregation of particles for the sequence-specific detection of DNA. ANALYST, 140(6), 2008-2015. doi:10.1039/c4an02101k

Xu, K., Begley, M. R., & Landers, J. P. (2015). Simultaneous metering and dispensing of multiple reagents on a passively controlled microdevice solely by finger pressing. LAB ON A CHIP, 15(3), 867-876. doi:10.1039/c4lc01319k

DuVall, J. A., Borba, J. C., Shafagati, N., Luzader, D., Shukla, N., Li, J., . . . Landers, J. P. (2015). Optical Imaging of Paramagnetic Bead-DNA Aggregation Inhibition Allows for Low Copy Number Detection of Infectious Pathogens. PLOS ONE, 10(6). doi:10.1371/journal.pone.0129830

2014

Le Roux, D., Root, B. E., Hickey, J. A., Scott, O. N., Tsuei, A., Li, J., . . . de Mazancourt, P. (2014). An integrated sample-in-answer-out microfluidic chip for rapid human identification by STR analysis. LAB ON A CHIP, 14(22), 4415-4425. doi:10.1039/c4lc00685b

Le Roux, D., Root, B. E., Reedy, C. R., Hickey, J. A., Scott, O. N., Bienvenue, J. M., . . . de Mazancourt, P. (2014). DNA Analysis Using an Integrated Microchip for Multiplex PCR Amplification and Electrophoresis for Reference Samples. ANALYTICAL CHEMISTRY, 86(16), 8192-8199. doi:10.1021/ac501666b

Nelson, D. A., Strachan, B. C., Sloane, H. S., Li, J., & Landers, J. P. (2014). Dual-force aggregation of magnetic particles enhances label-free quantification of DNA at the sub-single cell level. ANALYTICA CHIMICA ACTA, 819, 34-41. doi:10.1016/j.aca.2014.01.052

Liu, Q., Li, J., Liu, H., Tora, I., Ide, M. S., Lu, J., . . . Landers, J. P. (2014). Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles. NANO RESEARCH, 7(5), 755-764. doi:10.1007/s12274-014-0436-9

2013

Li, J., Liu, Q., Xiao, L., Haverstick, D. M., Dewald, A., Columbus, L., . . . Landers, J. P. (2013). Label-Free Method for Cell Counting in Crude Biological Samples via Paramagnetic Bead Aggregation. ANALYTICAL CHEMISTRY, 85(23), 11233-11239. doi:10.1021/ac401402h

Collino, R. R., Reilly-Shapiro, N., Foresman, B., Xu, K., Utz, M., Landers, J. P., & Begley, M. R. (2013). Flow switching in microfluidic networks using passive features and frequency tuning. LAB ON A CHIP, 13(18), 3668-3674. doi:10.1039/c3lc50481f

Lounsbury, J. A., Nambiar, S. M., Karlsson, A., Cunniffe, H., Norris, J. V., Ferrance, J. P., & Landers, J. P. (2014). Enhanced recovery of spermatozoa and comprehensive lysis of epithelial cells from sexual assault samples having a low cell counts or aged up to one year. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 8(1), 84-89. doi:10.1016/j.fsigen.2013.06.015

Marchiarullo, D. J., Sklavounos, A. H., Oh, K., Poe, B. L., Barker, N. S., & Landers, J. P. (2013). Low-power microwave-mediated heating for microchip-based PCR. LAB ON A CHIP, 13(17), 3417-3425. doi:10.1039/c3lc50461a

Durney, B. C., Lounsbury, J. A., Poe, B. L., Landers, J. P., & Holland, L. A. (2013). A Thermally Responsive Phospholipid Pseudogel: Tunable DNA Sieving with Capillary Electrophoresis. ANALYTICAL CHEMISTRY, 85(14), 6617-6625. doi:10.1021/ac303745g

Lounsbury, J. A., & Landers, J. P. (2013). Ultrafast Amplification of DNA on Plastic Microdevices for Forensic Short Tandem Repeat Analysis. JOURNAL OF FORENSIC SCIENCES, 58(4), 866-874. doi:10.1111/1556-4029.12162

Ouyang, Y., Wang, S., Li, J., Riehl, P. S., Begley, M., & Landers, J. P. (2013). Rapid patterning of 'tunable' hydrophobic valves on disposable microchips by laser printer lithography. LAB ON A CHIP, 13(9), 1762-1771. doi:10.1039/c3lc41275j

Lounsbury, J. A., Karlsson, A., Miranian, D. C., Cronk, S. M., Nelson, D. A., Li, J., . . . Landers, J. P. (2013). From sample to PCR product in under 45 minutes: a polymeric integrated microdevice for clinical and forensic DNA analysis. LAB ON A CHIP, 13(7), 1384-1393. doi:10.1039/c3lc41326h

Wu, J., Xu, K., Landers, J. P., & Weber, S. G. (2013). An in Situ Measurement of Extracellular Cysteamine, Homocysteine, and Cysteine Concentrations in Organotypic Hippocampal Slice Cultures by Integration of Electroosmotic Sampling and Microfluidic Analysis. ANALYTICAL CHEMISTRY, 85(6), 3095-3103. doi:10.1021/ac302676q

Li, J., Liu, Q., Alsamarri, H., Lounsbury, J. A., Haversitick, D. M., & Landers, J. P. (2013). Label-free DNA quantification via a 'pipette, aggregate and blot' (PAB) approach with magnetic silica particles on filter paper. LAB ON A CHIP, 13(5), 955-961. doi:10.1039/c2lc40975e

2012

Phaneuf, C. R., Oh, K., Pak, N., Saunders, D. C., Conrardy, C., Landers, J. P., . . . Forest, C. R. (2013). Sensitive, microliter PCR with consensus degenerate primers for Epstein Barr virus amplification. BIOMEDICAL MICRODEVICES, 15(2), 221-231. doi:10.1007/s10544-012-9720-1

Lounsbury, J. A., Bienvenue, J. M., & Landers, J. P. (2012). Sample-to-Result STR Genotyping Systems: Potential and Status.. Forensic science review, 24(2), 123-142.

Duarte, G. R. M., Coltro, W. K. T., Borba, J. C., Price, C. W., Landers, J. P., & Carrilho, E. (2012). Disposable polyester-toner electrophoresis microchips for DNA analysis. ANALYST, 137(11), 2692-2698. doi:10.1039/c2an16220b

Lounsbury, J. A., Coult, N., Miranian, D. C., Cronk, S. M., Haverstick, D. M., Kinnon, P., . . . Landers, J. P. (2012). An enzyme-based DNA preparation method for application to forensic biological samples and degraded stains. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 6(5), 607-615. doi:10.1016/j.fsigen.2012.01.011

Leslie, D. C., Li, J., Strachan, B. C., Begley, M. R., Finkler, D., Bazydlo, L. A. L., . . . Landers, J. P. (2012). New Detection Modality for Label-Free Quantification of DNA in Biological Samples via Superparamagnetic Bead Aggregation. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(12), 5689-5696. doi:10.1021/ja300839n

Poe, B. L., Haverstick, D. M., & Landers, J. P. (2012). Warfarin Genotyping in a Single PCR Reaction for Microchip Electrophoresis. CLINICAL CHEMISTRY, 58(4), 725-731. doi:10.1373/clinchem.2011.180356

Lounsbury, J. A., Poe, B. L., Do, M., & Landers, J. P. (2012). Laser-ablated poly(methyl methacrylate) microdevices for sub-microliter DNA amplification suitable for micro-total analysis systems. JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 22(8). doi:10.1088/0960-1317/22/8/085006

2011

Leslie, D. C., Seker, E., Bazydlo, L. A. L., Strachan, B. C., & Landers, J. P. (2012). Platinum nanoparticle-facilitated reflective surfaces for non-contact temperature control in microfluidic devices for PCR amplification. LAB ON A CHIP, 12(1), 127-132. doi:10.1039/c1lc20779b

Duarte, G. R. M., Price, C. W., Augustine, B. H., Carrilho, E., & Landers, J. P. (2011). Dynamic Solid Phase DNA Extraction and PCR Amplification in Polyester-Toner Based Microchip. ANALYTICAL CHEMISTRY, 83(13), 5182-5189. doi:10.1021/ac200292m

Hagan, K. A., Reedy, C. R., Bienvenue, J. M., Dewald, A. H., & Landers, J. P. (2011). A valveless microfluidic device for integrated solid phase extraction and polymerase chain reaction for short tandem repeat (STR) analysis. ANALYST, 136(9), 1928-1937. doi:10.1039/c0an00922a

Reedy, C. R., Price, C. W., Sniegowski, J., Ferrance, J. P., Begley, M., & Landers, J. P. (2011). Solid phase extraction of DNA from biological samples in a post-based, high surface area poly(methyl methacrylate) (PMMA) microdevice. LAB ON A CHIP, 11(9), 1603-1611. doi:10.1039/c0lc00597e

Cakal, C., Ferrance, J. P., Landers, J. P., & Caglar, P. (2011). Microchip extraction of catecholamines using a boronic acid functional affinity monolith. ANALYTICA CHIMICA ACTA, 690(1), 94-100. doi:10.1016/j.aca.2011.02.009

McIntosh, D., Zhou, Q., Lara, F. J., Landers, J., & Campbell, J. C. (2011). Fluorescence Detection 400-480nm Using Microfluidic System Integrated GaP Photodiodes. ADVANCES IN OPTOELECTRONICS, 2011. doi:10.1155/2011/491609

Reedy, C. R., Hagan, K. A., Marchiarullo, D. J., Dewald, A. H., Barron, A., Bienvenue, J. M., & Landers, J. P. (2011). A modular microfluidic system for deoxyribonucleic acid identification by short tandem repeat analysis (vol 687, pg 150, 2011). ANALYTICA CHIMICA ACTA, 699(1), 126. doi:10.1016/j.aca.2011.04.042

McIntosh, D., Zhou, Q., Lara, F. J., Landers, J., & Campbell, J. C. (2011). Flip-Chip Bonded GaP Photodiodes for Detection of 400-to 480-nm Fluorescence. IEEE PHOTONICS TECHNOLOGY LETTERS, 23(13), 878-880. doi:10.1109/LPT.2011.2141979

2010

Reedy, C. R., Hagan, K. A., Marchiarullo, D. J., Dewald, A. H., Barron, A., Bienvenue, J. M., & Landers, J. P. (2011). A modular microfluidic system for deoxyribonucleic acid identification by short tandem repeat analysis. ANALYTICA CHIMICA ACTA, 687(2), 150-158. doi:10.1016/j.aca.2010.12.016

Hagan, K. A., Reedy, C. R., Uchimoto, M. L., Basu, D., Engel, D. A., & Landers, J. P. (2011). An integrated, valveless system for microfluidic purification and reverse transcription-PCR amplification of RNA for detection of infectious agents. LAB ON A CHIP, 11(5), 957-961. doi:10.1039/c0lc00136h

Wu, J., Ferrance, J. P., Landers, J. P., & Weber, S. G. (2010). Integration of a Precolumn Fluorogenic Reaction, Separation, and Detection of Reduced Glutathione. ANALYTICAL CHEMISTRY, 82(17), 7267-7273. doi:10.1021/ac101182r

Leslie, D. C., Melnikoff, B. A., Marchiarullo, D. J., Cash, D. R., Ferrance, J. P., & Landers, J. P. (2010). A simple method for the evaluation of microfluidic architecture using flow quantitation via a multiplexed fluidic resistance measurement. LAB ON A CHIP, 10(15), 1960-1966. doi:10.1039/c003244a

Cakal, C., Ferrance, J. P., Landers, J. P., & Caglar, P. (2010). Development of a micro-total analysis system (μ-TAS) for the determination of catecholamines. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398(5), 1909-1917. doi:10.1007/s00216-010-3998-8

Bienvenue, J. M., & Landers, J. P. (2010). DNA Extraction on Microfluidic Devices.. Forensic science review, 22(2), 187-197.

Huang, L., Seker, E., Landers, J. P., Begley, M. R., & Utz, M. (2010). Molecular Interactions in Surface-Assembled Monolayers of Short Double-Stranded DNA. LANGMUIR, 26(13), 11574-11580. doi:10.1021/la100860d

Reedy, C. R., Hagan, K. A., Strachan, B. C., Higginson, J. J., Bienvenue, J. M., Greenspoon, S. A., . . . Landers, J. P. (2010). Dual-Domain Microchip-Based Process for Volume Reduction Solid Phase Extraction of Nucleic Acids from Dilute, Large Volume Biological Samples. ANALYTICAL CHEMISTRY, 82(13), 5669-5678. doi:10.1021/ac100649b

Leslie, D. C., Sohrabi, A., Ikonomi, P., McKee, M. L., & Landers, J. P. (2010). Size-based separations as an important discriminator in development of proximity ligation assays for protein or organism detection. ELECTROPHORESIS, 31(10), 1615-1622. doi:10.1002/elps.201000008

Duarte, G. R. M., Price, C. W., Littlewood, J. L., Haverstick, D. M., Ferrance, J. P., Carrilho, E., & Landers, J. P. (2010). Characterization of dynamic solid phase DNA extraction from blood with magnetically controlled silica beads. ANALYST, 135(3), 531-537. doi:10.1039/b918996c

Sofla, A., Seker, E., Landers, J. P., & Begley, M. R. (2010). PDMS-Glass Interface Adhesion Energy Determined Via Comprehensive Solutions for Thin Film Bulge/Blister Tests. JOURNAL OF APPLIED MECHANICS-TRANSACTIONS OF THE ASME, 77(3). doi:10.1115/1.4000428