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Marie-Louise Hammarskjold

Marie-Louise Hammarskjold

Professor, Microbiology, Immunology, and Cancer Biology

Primary Location
PO Box 800734
Pinn Hall 7086
Primary Phone Number
434.982.1598
Email
[email protected]

Bio & Overview

Many mammalian and viral genes are alternatively spliced and subject to regulation at the post- transcriptional level. However, relatively little is known about the cellular mechanisms for this regulation. We are using retroviruses as model systems to elucidate these mechanisms. Some of our studies focus on HIV Rev, an essential HIV protein. Rev mediates the nucleo-cytoplasmic export of unspliced and incompletely spliced HIV RNAs and provides an important HIV drug target. Another major focus of the laboratory is the function of the constitutive transport element (CTE). The CTE interacts directly with host cell proteins to facilitate export of intron containing RNA. We are currently analyzing the function of cellular proteins that are involved in the CTE mediated export pathway. One is NXF1 (TAP), a protein which has been proposed to play an essential role in cellular mRNA export. A second protein under study is NXT1, an important TAP cofactor. CTE function is also enhanced by SAM68, a major target of Src and Src family kinases and a potential tumor suppressor. Our studies are aimed at identifying the mechanism by which SAM68 promotes CTE function and the role that this protein plays in cellular gene regulation.

Academic Information

Department
Microbiology, Immunology, and Cancer Biology
Academic Title
Professor

Primary Education

  • BS, Chemistry, University of Stockholm, Stockholm, Sweden, 1967
  • MS, Biochemistry, University of Stockholm, Stockholm, Sweden, 1971
  • MD, Medicine, Karolinska Institute, Stockholm, Sweden, 1977
  • PhD, Medical Microbiology, Karolinska Institute, Stockholm, Sweden, 1981
  • Postdoc, Medical Microbiology, SUNY at Stony Brook, 1983

Other Appointments

  • Professor, MII
  • Professor, BMBG
  • Professor, MOMD

Other Affiliations

  • Member, Molecular Genetics and Epigenetics Program
  • Professor, BIMS
  • Associate Director, Myles H. Thaler Center

Research Interests

Post Transcriptional Gene Regulation and the Molecular Biology of Human Retroviruses

Research Disciplines

  • Biotechnology
  • Cancer Biology
  • Immunology
  • Infectious Diseases/Biodefense

Research Opportunities

  • Biotechnology
  • Cancer Biology
  • Immunology
  • Infectious Diseases

PhD Programs

  • Biochemistry & Molecular Genetics
  • Microbiology

Selected Publications

2023

Jackson, P. E. H., Holsey, J., Turse, L., Hammarskjold, M. -L., & Rekosh, D. (2023). Rev-Rev Response Element Activity Selection Bias at the Human Immunodeficiency Virus Transmission Bottleneck. OPEN FORUM INFECTIOUS DISEASES, 10(10). doi:10.1093/ofid/ofad486

Jackson, P. E. H., Holsey, J., Turse, L., Marie-Louise, H., & Rekosh, D. (2023). Rev-Rev Response Element Activity Selection Bias at the HIV Transmission Bottleneck.. bioRxiv. doi:10.1101/2023.04.05.535732

2022

Dzhivhuho, G., Holsey, J., Honeycutt, E., O'Farrell, H., Rekosh, D., Hammarskjold, M. -L., & Jackson, P. E. H. (2022). HIV-1 Rev-RRE functional activity in primary isolates is highly dependent on minimal context-dependent changes in Rev. SCIENTIFIC REPORTS, 12(1). doi:10.1038/s41598-022-21714-2

Cronk, J. M., Dziewulska, K. H., Puchalski, P., Crittenden, R. B., Hammarskjod, M. -L., & Brown, M. G. (2022). Altered-Self MHC Class I Sensing via Functionally Disparate Paired NK Cell Receptors Counters Murine Cytomegalovirus gp34-Mediated Immune Evasion. JOURNAL OF IMMUNOLOGY, 209(8), 1545-1554. doi:10.4049/jimmunol.2200441

2020

Grabski, D. F., Broseus, L., Kumari, B., Rekosh, D., Hammarskjold, M. -L., & Ritchie, W. (2021). Intron retention and its impact on gene expression and protein diversity: A review and a practical guide. WILEY INTERDISCIPLINARY REVIEWS-RNA, 12(1). doi:10.1002/wrna.1631

Grabski, D. F., Ratan, A., Gray, L. R., Bekiranov, S., Rekosh, D., Hammarskjold, M. -L., & Rasmussen, S. K. (2021). Upregulation of human endogenous retrovirus-K (HML-2) mRNAs in hepatoblastoma: Identification of potential new immunotherapeutic targets and biomarkers. JOURNAL OF PEDIATRIC SURGERY, 56(2), 286-292. doi:10.1016/j.jpedsurg.2020.05.022

Mavhandu, L. G., Cheng, H., Bor, Y. -C., Tebit, D. M., Hammarskjold, M. -L., Rekosh, D., & Bessong, P. O. (2020). Development of a pseudovirus assay and evaluation to screen natural products for inhibition of HIV-1 subtype C reverse transcriptase. JOURNAL OF ETHNOPHARMACOLOGY, 258. doi:10.1016/j.jep.2020.112931

Jackson, K. N., Kahler, D. M., Kucharska, I., Rekosh, D., Hammarskjold, M. -L., & Smith, J. A. (2020). Inactivation of MS2 Bacteriophage and Adenovirus with Silver and Copper in Solution and Embedded in Ceramic Water Filters. JOURNAL OF ENVIRONMENTAL ENGINEERING, 146(3). doi:10.1061/(ASCE)EE.1943-7870.0001634

Jackson, P. E. H., Dzhivhuho, G., Rekosh, D., & Hammarskjold, M. -L. (2020). Sequence and Functional Variation in the HIV-1 Rev Regulatory Axis. CURRENT HIV RESEARCH, 18(2), 85-98. doi:10.2174/1570162X18666200106112842

2019

Gamache, A., Cronk, J. M., Nash, W. T., Puchalski, P., Gillespie, A., Wei, H., . . . Brown, M. G. (2019). Ly49R activation receptor drives self-MHC-educated NK cell immunity against cytomegalovirus infection. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 116(52), 26768-26778. doi:10.1073/pnas.1913064117

Gray, L. R., Jackson, R. E., Jackson, P. E. H., Bekiranov, S., Rekosh, D., & Hammarskjold, M. -L. (2019). HIV-1 Rev interacts with HERV-K RcREs present in the human genome and promotes export of unspliced HERV-K proviral RNA. RETROVIROLOGY, 16(1). doi:10.1186/s12977-019-0505-y

Sherpa, C., Jackson, P. E. H., Gray, L. R., Anastos, K., Le Grice, S. F. J., Hammarskjold, M. -L., & Rekosh, D. (2019). Evolution of the HIV-1 Rev Response Element during Natural Infection Reveals Nucleotide Changes That Correlate with Altered Structure and Increased Activity over Time. JOURNAL OF VIROLOGY, 93(11). doi:10.1128/JVI.02102-18

Jackson, P. E. H., Huang, J., Sharma, M., Rasmussen, S. K., Hammarskjold, M. -L., & Rekosh, D. (2019). A novel retroviral vector system to analyze expression from mRNA with retained introns using fluorescent proteins and flow cytometry. SCIENTIFIC REPORTS, 9. doi:10.1038/s41598-019-42914-3

Matume, N. D., Tebit, D. M., Gray, L. R., Turner, S. D., Rekosh, D., Bessong, P. O., & Hammarskjold, M. -L. (2019). Characterization of APOBEC3 variation in a population of HIV-1 infected individuals in northern South Africa. BMC MEDICAL GENETICS, 20. doi:10.1186/s12881-018-0740-4

2018

Adelman, Z. N., Albritton, L. M., Boris-Lawrie, K., Buchmeier, M. J., Cannon, P., Cho, M., . . . Zoloth, L. (2018). Protect NIH's DNA advisory committee. SCIENCE, 362(6413), 409-410. doi:10.1126/science.aav2483

Rekosh, D., & Hammarskjold, M. -L. (2018). Intron retention in viruses and cellular genes: Detention, border controls and passports. WILEY INTERDISCIPLINARY REVIEWS-RNA, 9(3). doi:10.1002/wrna.1470

Matume, N. D., Tebit, D. M., Gray, L. R., Hammarskjold, M. -L., Rekosh, D., & Bessong, P. O. (2018). Next generation sequencing reveals a high frequency of CXCR4 utilizing viruses in HIV-1 chronically infected drug experienced individuals in South Africa. JOURNAL OF CLINICAL VIROLOGY, 103, 81-87. doi:10.1016/j.jcv.2018.02.008

2017

Hammarskjold, M. -L., & Rekosh, D. (2017). SR proteins: To shuttle or not to shuttle, that is the question. JOURNAL OF CELL BIOLOGY, 216(7), 1875-1877. doi:10.1083/jcb.201705009

2016

Li, Y., Bor, Y. -C., Fitzgerald, M. P., Lee, K. S., Rekosh, D., & Hammarskjold, M. -L. (2016). An NXF1 mRNA with a retained intron is expressed in hippocampal and neocortical neurons and is translated into a protein that functions as an Nxf1 cofactor. MOLECULAR BIOLOGY OF THE CELL, 27(24), 3903-3912. doi:10.1091/mbc.E16-07-0515

Wynn, J. E., Zhang, W., Tebit, D. M., Gray, L. R., Hammarskjold, M. -L., Rekosh, D., & Santos, W. L. (2016). Effect of intercalator and Lewis acid-base branched peptide complex formation: boosting affinity towards HIV-1 RRE RNA. MEDCHEMCOMM, 7(7), 1436-1440. doi:10.1039/c6md00171h

Jackson, P. E., Tebit, D. M., Rekosh, D., & Hammarskjold, M. -L. (2016). Rev-RRE Functional Activity Differs Substantially Among Primary HIV-1 Isolates. AIDS RESEARCH AND HUMAN RETROVIRUSES, 32(9), 923-934. doi:10.1089/aid.2016.0047

Wynn, J. E., Zhang, W., Tebit, D. M., Gray, L. R., Hammarskjold, M. -L., Rekosh, D., & Santos, W. L. (2016). Characterization and in vitro activity of a branched peptide boronic acid that interacts with HIV-1 RRE RNA. BIOORGANIC & MEDICINAL CHEMISTRY, 24(17), 3947-3952. doi:10.1016/j.bmc.2016.04.009

2015

Sherpa, C., Rausch, J. W., Le Grice, S. F. J., Hammarskjold, M. -L., & Rekosh, D. (2015). The HIV-1 Rev response element (RRE) adopts alternative conformations that promote different rates of virus replication. NUCLEIC ACIDS RESEARCH, 43(9), 4676-4686. doi:10.1093/nar/gkv313

Hammarskjoeld, M. -L. (2015). RNA and lessons from viruses. RNA, 21(4), 632-633. doi:10.1261/rna.050310.115

Wang, B., Rekosh, D., & Hammarskjold, M. -L. (2015). Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns. RNA, 21(3), 426-437. doi:10.1261/rna.048520.114

2013

Sloan, E. A., Kearney, M. F., Gray, L. R., Anastos, K., Daar, E. S., Margolick, J., . . . Rekosh, D. (2013). Limited Nucleotide Changes in the Rev Response Element (RRE) during HIV-1 Infection Alter Overall Rev-RRE Activity and Rev Multimerization. JOURNAL OF VIROLOGY, 87(20), 11173-11186. doi:10.1128/JVI.01392-13

2011

Iweriebor, B. C., Mavhandu, L. G., Masebe, T., Rekosh, D., Hammarskjold, M. -L., Mphahlele, J. M., & Bessong, P. O. (2012). Molecular epidemiology of HIV in two highly endemic areas of northeastern South Africa. ARCHIVES OF VIROLOGY, 157(3), 455-465. doi:10.1007/s00705-011-1180-z

Coyle, J. H., Bor, Y. -C., Rekosh, D., & Hammarskjold, M. -L. (2011). The Tpr protein regulates export of mRNAs with retained introns that traffic through the Nxf1 pathway. RNA, 17(7), 1344-1356. doi:10.1261/rna.2616111

Hammarskjold, M. -L., & Rekosh, D. (2011). A Long-Awaited Structure Is Rev-ealed. VIRUSES-BASEL, 3(5), 484-492. doi:10.3390/v3050484

Shuck-Lee, D., Chang, H., Sloan, E. A., Hammarskjold, M. -L., & Rekosh, D. (2011). Single-Nucleotide Changes in the HIV Rev-Response Element Mediate Resistance to Compounds That Inhibit Rev Function. JOURNAL OF VIROLOGY, 85(8), 3940-3949. doi:10.1128/JVI.02683-10