Cytek Aurora Borealis

The Cytek Aurora is equipped with five lasers – blue (488nm – 50mW), violet (405nm – 100mW), yellow-green (561nm – 50mW), red (640nm – 80mW) and UV (355nm – 20mW), three scattering channels, up to 64 fluorescence detectors and a plate loader. Spectral fingerprints of each fluorochrome are recorded and then unmixed in a highly multicolor experiment. As a result of this approach, combinations of fluorochromes that previously were incompatible to run together by conventional flow cytometric methods are now possible with this technology. Running CFSE, GFP, and A488 or DAPI, BV421, and Pacific Blue are no longer a problem. As long as a fluorochrome’s spectral fingerprint is distinct from another they can be unmixed.  The Automatic Micro-Sampling System (AMS) utilizes 96 well plate acquisition for maximum productivity.

Click here for a panel design grid to help design your antibody panels for the Cytek Aurora Borealis.

Click here to evaluate and optimize your panel design using the Cytek Full Spectrum Viewer.

Additional resources:

Aurora 5-Laser Panel Guide

Optimized Multicolor Immunofluorescence Panel (OMIP) is a special peer-reviewed Cytometry Part A publication type that reports on newly designed and optimized multicolor panels for flow cytometry.