{"id":1651,"date":"2018-01-18T16:36:19","date_gmt":"2018-01-18T21:36:19","guid":{"rendered":"https:\/\/med.virginia.edu\/gatc\/?page_id=1651"},"modified":"2022-11-22T14:42:08","modified_gmt":"2022-11-22T19:42:08","slug":"sample-requirements","status":"publish","type":"page","link":"https:\/\/med.virginia.edu\/gatc\/services\/sample-requirements\/","title":{"rendered":"Sample Requirements"},"content":{"rendered":"

To help ensure you get the quality of data you expect, please follow the sample requirements below.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Service<\/th>\nRequired sample volume<\/th>\nSample concentration<\/th>\nSample container<\/th>\nComments<\/th>\n<\/tr>\n
NanoVue – RNA or DNA<\/span><\/td>\n2.5 \u00b5L<\/td>\n\u00a05 ng\/\u00b5L or higher<\/td>\n1.5 ml tube<\/td>\n<\/td>\n<\/tr>\n
Qubit – DNA HS<\/span><\/td>\n2 \u00b5L<\/td>\n0.2 – 100 ng\/\u00b5L<\/td>\n1.5 ml tube<\/td>\n<\/td>\n<\/tr>\n
Qubit – RNA HS<\/td>\n2 \u00b5L<\/td>\n5 – 100 ng\/\u00b5L<\/td>\n1.5 ml tube<\/td>\n<\/td>\n<\/tr>\n
Agilent – Tape Station – RNA kit<\/td>\n2 \u00b5L<\/td>\n25 \u2013 500 ng\/\u03bcL<\/td>\n8 tube strip or 96 well plate<\/td>\n<\/td>\n<\/tr>\n
Agilent – Tape Station – RNA HS kit<\/td>\n3 \u00b5L<\/td>\n0.5 \u2013 10 ng\/\u03bcL<\/td>\n8 tube strip or 96 well plate<\/td>\n<\/td>\n<\/tr>\n
Agilent – Tape Station – DNA5000 HS kit<\/td>\n3 \u00b5L<\/td>\n100 \u2013 5000 bp, 10\u00a0\u2013\u00a01000 pg\/\u03bcL<\/td>\n8 tube strip or 96 well plate<\/td>\n<\/td>\n<\/tr>\n
Agilent – Bioanalyzer – DNA HS<\/td>\n2 \u00b5L<\/td>\n50\u00a0\u2013\u00a07000 bp, 5\u00a0\u2013\u00a0500 pg\/\u03bcL<\/td>\n1.5 ml tube<\/td>\n<\/td>\n<\/tr>\n
Pippin Prep – core run per sample<\/td>\nup to 30\u00a0\u00b5L<\/td>\n5 ng – 10 \u00b5g of material<\/td>\n1.5 ml tube<\/td>\n<\/td>\n<\/tr>\n
NGS library prep – 10X Genomics – single cell RNAseq<\/td>\n1 ml<\/td>\n500 cells\/ \u00b5L in PBS + 0.04% non-acetylated BSA<\/td>\nWash and resuspend in 1.5ml low binding tube<\/td>\n\u00a0Use wide bore low binding pipet tips, see core for specifics<\/td>\n<\/tr>\n
NGS library prep – RNAseq – mRNA isolation<\/td>\n\u00a0up to 50\u00a0\u00b5L<\/td>\n100 ng – 1 \u00b5g in up to 50 \u00b5L, 1 \u00b5g preferred (20ng\/\u00b5L)<\/td>\n8 tube strip or 96 well plate<\/td>\n\u00a0Additional material for NanoVue and Agilent REQUIRED<\/td>\n<\/tr>\n
NGS library prep – RNAseq – rRNA depletion<\/td>\nup to 12\u00a0\u00b5L<\/td>\n100 ng – 1 \u00b5g in up to 12 \u00b5L, 1 \u00b5g preferred (83 ng\/\u00b5L)<\/td>\n8 tube strip or 96 well plate<\/td>\n\u00a0Additional material for NanoVue and Agilent REQUIRED<\/td>\n<\/tr>\n
\u00a0NGS Illumina NextSeq Run Setup<\/td>\n\u00a018.2 \u00b5L – 5\u00a0\u00b5L<\/td>\n\u00a01.1 nM – 4 nM<\/td>\n\u00a01.5 ml tube<\/td>\n\u00a0This does not leave any excess for QC – additional volume for Qubit, Agilent & qPCR recommended<\/td>\n<\/tr>\n
\u00a0NGS Illumina MiSeq Run Setup<\/td>\n\u00a018.2 \u00b5L – 5\u00a0\u00b5L<\/td>\n\u00a01.1 nM – 4 nM<\/td>\n\u00a01.5 ml tube<\/td>\n\u00a0This does not leave any excess for QC – additional volume for Qubit, Agilent & qPCR recommended<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/h4>\n","protected":false},"excerpt":{"rendered":"

To help ensure you get the quality of data you expect, please follow the sample requirements below. Service Required sample volume Sample concentration Sample container Comments NanoVue – RNA or DNA 2.5 \u00b5L \u00a05 ng\/\u00b5L or higher 1.5 ml tube Qubit – DNA HS 2 \u00b5L 0.2 – 100 ng\/\u00b5L 1.5 ml tube Qubit – […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":13,"menu_order":30,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"tags":[],"class_list":["post-1651","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nSample Requirements - Genome Analysis and Technology Core<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/gatc\/services\/sample-requirements\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Sample Requirements - Genome Analysis and Technology Core\" \/>\n<meta property=\"og:description\" content=\"To help ensure you get the quality of data you expect, please follow the sample requirements below. 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