Congenic Mouse

Speed Congenics

Traditional development of a congenic strain, which involves serial backcrossing to transfer a gene (allele) from a donor strain to a recipient inbred strain, requires 2.5 to 3 years to complete 10 generations of backcrossing (N10).  In contrast, speed congenic strain development relies on microsatellite marker difference (simple sequence length polymorphisms, SSLPs) or SNPs between strains by selective breeding of progeny with higher percentage of recipient genome, thereby shortening the generations of backcrossing to 5 (N5, 1.25 to 1.5 years).  In addition, genome scanning can be performed with a panel of mapped SSLP makers or SNPs for not only (1) introgressing target loci onto selected inbred backgrounds, but also (2) linkage analysis and (3) characterizing genetic backgrounds. Please contact GEMM if you need this service.