Mouse in vitro Fertilization (IVF)

When a male mouse repeatedly fails to plug or impregnate normal female mice due to unknown reasons, IVF is the preferred first choice of methods to overcome male fertility problems.  However, the success of IVF relies largely on that reasonable number of motile sperm can be retrieved.  This is often unknown until after the male mouse is euthanized, the sperm collected from cauda epididymides, and the sperm number and motility assessed under the microscope.  If an insufficient number of sperm and/or immotile sperm are found, the investigators will be informed on the day of the procedure and, if IVF is cancelled, a responsible charge levied for the service provided.  If a sufficient number of motile sperm are obtained, the transgenic facility will perform to produce offspring.