Transgene Detection Methods
- PCR primers should be designed to be transgene-specific, usually spanning a novel promoter-gene junction.
- Southern blot digestions should be similarly designed to give a transgene-specific band, distinguishable from possible endogenous bands if the gene is mouse or mammal-derived.
- In this example, the upper bands are endogenous mouse bands, and the lower band is transgene-specific. Note that the endogenous bands are single-copy in intensity, and the transgene band increases in intensity in proportion to how many copies are spiked into the mouse genomic DNA.