{"id":442,"date":"2015-08-17T14:11:24","date_gmt":"2015-08-17T18:11:24","guid":{"rendered":"https:\/\/med.virginia.edu\/genetically-engineered-murine-model-core\/?page_id=442"},"modified":"2025-10-09T13:27:42","modified_gmt":"2025-10-09T17:27:42","slug":"research","status":"publish","type":"page","link":"https:\/\/med.virginia.edu\/genetically-engineered-murine-model-core\/research\/","title":{"rendered":"Resources and References"},"content":{"rendered":"<h2 class=\"post-title\">Mice Strains Used in GEMM<\/h2>\n<h3>Transgene injections:<\/h3>\n<p>B6SJLF1\/J, (C57BL\/6 Female X\u00a0SJL Male), agouti, Jackson Lab Stock #100012<\/p>\n<p>Transgenic\u00a0founders\u00a0are B6SJLF2.<\/p>\n<p>This is the default strain we will use unless otherwise requested.<\/p>\n<h5>The inbred strains are generally\u00a0less efficient for production of transgenic mice, and will therefore be more costly for you.<\/h5>\n<p>C57Bl\/6NHsd, non-agouti (black) inbred, abbrev. B6N, Envigo (formerly Harlan)<\/p>\n<p>C57BL\/6J, non-agouti (black) inbred, abbrev. B6J, Jackson Lab Stock #000664<\/p>\n<hr \/>\n<h3>ES cell injections:<\/h3>\n<p>For 129, agouti B6 or C57BL\/6J-Tyr<sup>c-2J<\/sup>\u00a0ES cells:<\/p>\n<p>B6D2F1N1, (C57BL\/6 Female\u00a0x B6D2F1 Male), black, Jackson Lab Stock #000664 x #100006<\/p>\n<p>Diagram of 129\/Sv ES cells into B6 blastocyst<\/p>\n<p>For black B6 ES cells:<\/p>\n<p>C57BL\/6J-Tyr<sup>c-2J<\/sup>, albino, Harlan Stock \u201cC57BL\/6 Albino Mice\u201d or Jackson Lab Stock #000058<\/p>\n<p>BALB\/cJ, albino, Jackson Lab Stock #000651<\/p>\n<hr \/>\n<h3 class=\"post-title\">Pattern for ear punch numbering system<\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-249 size-medium\" src=\"https:\/\/med.virginia.edu\/genetically-engineered-murine-model-core\/wp-content\/uploads\/sites\/172\/2015\/08\/EARNotches2-300x211.jpg\" alt=\"Pattern for ear punch numbering system\" width=\"300\" height=\"211\" srcset=\"https:\/\/med.virginia.edu\/genetically-engineered-murine-model-core\/wp-content\/uploads\/sites\/172\/2015\/08\/EARNotches2-300x211.jpg 300w, https:\/\/med.virginia.edu\/genetically-engineered-murine-model-core\/wp-content\/uploads\/sites\/172\/2015\/08\/EARNotches2.jpg 470w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p>Drawn by William Ober (2001)<\/p>\n<p>From page 86, Figure 31 in Manipulating the Mouse Embryo, A Laboratory Manual, 1986, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Mice Strains Used in GEMM Transgene injections: B6SJLF1\/J, (C57BL\/6 Female X\u00a0SJL Male), agouti, Jackson Lab Stock #100012 Transgenic\u00a0founders\u00a0are B6SJLF2. This is the default strain we will use unless otherwise requested. The inbred strains are generally\u00a0less efficient for production of transgenic mice, and will therefore be more costly for you. C57Bl\/6NHsd, non-agouti (black) inbred, abbrev. B6N, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":6,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"tags":[],"class_list":["post-442","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Resources and References - Genetically Engineered Murine Model (GEMM) Core<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/genetically-engineered-murine-model-core\/research\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Resources and References - Genetically Engineered Murine Model (GEMM) Core\" \/>\n<meta property=\"og:description\" content=\"Mice Strains Used in GEMM Transgene injections: B6SJLF1\/J, (C57BL\/6 Female X\u00a0SJL Male), agouti, Jackson Lab Stock #100012 Transgenic\u00a0founders\u00a0are B6SJLF2. This is the default strain we will use unless otherwise requested. The inbred strains are generally\u00a0less efficient for production of transgenic mice, and will therefore be more costly for you. C57Bl\/6NHsd, non-agouti (black) inbred, abbrev. 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