Image and Video Gallery
Microglial process extension in response to glutamate in brain slices
Microglia in a hippocampal brain slice of a CX3CR1-GFP expressing mouse extend their processes toward the region of neuronal cell bodies during glutamate (1mM) treatment. Time is displayed as hr:min. Read more in Eyo UB, Peng J, Przemyslaw S, Mukherjee A, Bispo A, Wu LJ (2014). Neuronal Hyperactivity Recruits Microglial Processes via Neuronal NMDA Receptors and Microglial P2Y12 Receptors after Status Epilepticus. Journal of Neuroscience, 34 (32): 10528-10540.
Microglial process convergence towards a neuronal dendrite in response to glutamate in brain slices
Microglial (green) processes in a brain slice of a CX3CR1-GFP expressing mouse converge on a neuronal dendrite (red) after glutamate (1mM) treatment. Time is displayed as hr:min. Read more in Eyo UB, Peng, J, Murugan M, Mo M, Lalani A, Xie P, Xu P, Margolis DJ, Wu LJ (2017). Regulation of Physical Microglia-Neuron Interactions by Fractalkine Signaling after Status Epilepticus. eNeuro. doi: 10.1523/ENEURO.0209-16.2016.
Microglial process convergence in response to extracellular calcium depletion in vivo
Microglial processes in the somatosensory cortex of a CX3CR1-GFP expressing mouse converge at certain “hotspot” when bathed in Ca2+-deficient ACSF with 2mM EGTA to eliminate the extracellular calcium concentrations. Time is displayed as hr:min. Read more in Eyo UB, Gu N, De S, Dong H, Richardson JR, Wu LJ (2015). Modulation of Microglial Process Convergence towards Neuronal Dendrites by Extracellular Calcium. Journal of Neuroscience, 35(6): 2417-2422.
Microglial landscape changes in by translocation in vivo
Microglial cells (four cells tracked with various colors) translocate to varying degrees through the naïve brain over several days. Read more in Eyo UB, Mo M, Yi M-H , Murugan M, Liu J, Yarlagadda R, Margolis DJ, Xu P, Wu LJ (2018). P2Y12R-Dependent Translocation Mechanisms Gate the Changing Microglial Landscape. Cell Rep. Apr 24; 23(4):979-966. doi: 1016/j.celrep.2018.04.001.