{"id":720,"date":"2016-12-06T17:17:30","date_gmt":"2016-12-06T17:17:30","guid":{"rendered":"https:\/\/research.med.virginia.edu\/pcen\/?page_id=720"},"modified":"2016-12-06T17:17:30","modified_gmt":"2016-12-06T17:17:30","slug":"training-offered","status":"publish","type":"page","link":"https:\/\/med.virginia.edu\/pcen\/enrichment-program\/training-offered\/","title":{"rendered":"Training Offered"},"content":{"rendered":"<h2>Microsurgical procedures in mice<\/h2>\n<h3><em>Contact: Dr. Sequeira Lopez &#8211;\u00a0<a href=\"mailto:msl7u@virginia.edu\">msl7u@virginia.edu<\/a><\/em><\/h3>\n<ul>\n<li>Acute central blood pressure measurement.<\/li>\n<li>Ambulatory radio telemetry blood pressure, temperature and ECG recordings (with DSI units).<\/li>\n<li>Partial and total unilateral ureteral obstruction and release in neonatal and adult mice.<\/li>\n<li>Complete unilateral ureteral obstruction in adult mice.<\/li>\n<li>Transplantation of embryonic organs under the kidney capsule.<\/li>\n<li>Nephrectomy.<\/li>\n<li>Kidney ischemia reperfusion injury.<\/li>\n<li>Bone marrow transplantation.<\/li>\n<\/ul>\n<hr \/>\n<h2>Cell differentiation<\/h2>\n<h3><em>Contact: Dr. Sequeira Lopez &#8211;\u00a0<a href=\"mailto:msl7u@virginia.edu\">msl7u@virginia.edu<\/a><\/em><\/h3>\n<ul>\n<li>In vitro colony forming assays of hematopoietic cells.<\/li>\n<\/ul>\n<hr \/>\n<h2>Histology<\/h2>\n<p>We provide training protocols and assistance<\/p>\n<h3><em>Contact: Dr. Sequeira Lopez &#8211;\u00a0<a href=\"mailto:msl7u@virginia.edu\">msl7u@virginia.edu<\/a><\/em><\/h3>\n<ul>\n<li>Tissue harvesting<\/li>\n<li>Tissue embedding<\/li>\n<li>Paraffin and Frozen sections<\/li>\n<li>Histochemical stainings (Hematoxylin and eosin, PAS, Mason\u2019s Trichrome, Sirius Red, etc..)<\/li>\n<li>Immunostaining<\/li>\n<li><em>In situ<\/em> hybridization<\/li>\n<li><em>Clearing techniques<\/em><\/li>\n<li>Microdissection<\/li>\n<li>Analysis and quantification using Metamorph<\/li>\n<\/ul>\n<hr \/>\n<h2>Mouse breeding strategies<\/h2>\n<h3><em>Contact: Dr. Sequeira Lopez &#8211;\u00a0<a href=\"mailto:msl7u@virginia.edu\">msl7u@virginia.edu<\/a><\/em><\/h3>\n<hr \/>\n<h2>Cre Lox lineage tracing strategies<\/h2>\n<h3><em>Contact: Dr. Sequeira Lopez &#8211;\u00a0<a href=\"mailto:msl7u@virginia.edu\">msl7u@virginia.edu<\/a><\/em><\/h3>\n<hr \/>\n<h2>Advice for experimental planning using molecular approaches<\/h2>\n<h3><em>Contact: Dr. Medrano &#8211;\u00a0<a href=\"mailto:sm8z@virginia.edu\">sm8z@virginia.edu<\/a><\/em><\/h3>\n<ul>\n<li>Cloning<\/li>\n<li>CRISPR technology<\/li>\n<li>Targeting design<\/li>\n<li>Single Cell Genomics<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Microsurgical procedures in mice Contact: Dr. Sequeira Lopez &#8211;\u00a0msl7u@virginia.edu Acute central blood pressure measurement. Ambulatory radio telemetry blood pressure, temperature and ECG recordings (with DSI units). Partial and total unilateral ureteral obstruction and release in neonatal and adult mice. Complete unilateral ureteral obstruction in adult mice. Transplantation of embryonic organs under the kidney capsule. Nephrectomy. [&hellip;]<\/p>\n","protected":false},"author":1777,"featured_media":0,"parent":76,"menu_order":27,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"tags":[],"class_list":["post-720","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.1.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Training Offered - Pediatric Center of Excellence<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/med.virginia.edu\/pcen\/enrichment-program\/training-offered\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Training Offered - Pediatric Center of Excellence\" \/>\n<meta property=\"og:description\" content=\"Microsurgical procedures in mice Contact: Dr. Sequeira Lopez &#8211;\u00a0msl7u@virginia.edu Acute central blood pressure measurement. Ambulatory radio telemetry blood pressure, temperature and ECG recordings (with DSI units). Partial and total unilateral ureteral obstruction and release in neonatal and adult mice. Complete unilateral ureteral obstruction in adult mice. Transplantation of embryonic organs under the kidney capsule. Nephrectomy. 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