Methods and Resources

The UVa Molecular Electron Microscopy Core laboratory is committed to transmission electron microscopy of samples larger than 250 KDa, with the goal of image reconstruction at better than 1nm resolution. The facility’s three electron microscopes provide a progression of capability, from the Tecnai Spirit for initial screening of negative-stained samples, to the Tecnai F20 for preliminary electron cryomicroscopy, to the advanced Titan Krios, which is dedicated to automated data collection for high-resolution analysis.

Kelly Dryden – Core Director and Assistant Professor of Research in Molecular Biology and Biological Physics

The nuclear magnetic resonance (NMR) spectroscopy facility provides information on molecular structure and dynamics at atomic level resolution. The biomolecular nuclear magnetic resonance facility at UVa is home to one 800, four 600, and one 500 MHz spectrometers, which are equipped with triple-resonance cryoprobes. A 500 MHz solid-state NMR spectrometer with a heteronuclear MAS-probe is available for solids experiments. EPR equipment includes two X-band cw spectrometers, a pulse X-band machine capable for DEER, and a pulse Q band spectrometer.