Media, Cells and Reagents

The catalog of items available from the Tissue Culture Facility is available through iLab ( To see the catalog and purchase items, you need to have an account in iLab and have access to a PTAEO. See:

On the MolMart Catalog tab, you can search for products by name or catalog number. The UNSPSC Category search is not yet fully implemented so you may not some stocked items with a category search.

To purchase items, come to room 226 Pinn Hall  9 a.m. to 5 p.m. M-F.

  • Find media in cold cabinets, buffers and pipet tips in cabinets against wall nearest to hall.
  • check out items at a self service station, see instructions
  • Bills will be sent the next month.

The items in the Tissue Culture Facility are available only to researchers at the University of Virginia.


The media below are supplied by Invitrogen

Description Invitrogen Catalog
Basal Media
DMEM (high glucose) [with 4500 mg/ml D-glucose, L-glutamine, without sodium pyruvate] 11965-092 500 ml
DMEM (low glucose) [with 1000 mg/ml D-glucose, 110 mg/L sodium pyruvate, without L-glutamine, phenol red] 11054-020 500 ml
DMEM (low glucose) [with 1000 mg/ml D-glucose, L- glutamine, 110 mg/L sodium pyruvate] 11885-084 500 ml
DMEM/F-12 (1:1) [with 15 mM HEPES, L-glutamine] 11330-032 500 ml
DMEM/F-12 (1:1) [with L-glutamine, 15 mM HEPES, pyridoxine-HCl, without phenol red] 11039-021 500 ml
DMEM/F-12 (1:1) [with L-glutamine, without HEPES] 11320-033 500 ml
F-12 Nutrient Mixture (Ham’s) [with L-glutamine] 11765-054 500 ml
Grace’s Insect Medium, supplemented 11605-094 500 ml
IMDM [with L-glutamine, 25 mM HEPES, 3024 mg/L sodium bicarbonate, without α-thioglycerol, β-mercaptoethanol] 12440-046 1000 ml
IMDM [with L-glutamine, 25 mM HEPES, 3024 mg/L sodium bicarbonate, without α-thioglycerol, β-mercaptoethanol] 12440-053 500 ml
McCoy’s 5A Medium (modified) [with L-glutamine] 16600-082 500 ml
Medium 199 [with Earle’s salts, L-glutamine, 2200 mg/L sodium bicarbonate] 11150-059 500 ml
MEM [with Earle’s salts, L-glutamine] 11095-080 500 ml
MEM [with Earle’s salts, non-essential amino acids, without L-glutamine] 10370-021 500 ml
Opti-MEM I, reduced serum medium [with HEPES, 2400 mg/L sodium bicarbonate, hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements, growth factors, phenol red reduced to 1.1 mg/L] 31985-070 500 ml
RPMI Medium 1640 [with L-glutamine, without phenol red] 11835-030 500 ml
RPMI Medium 1640 [with L-glutamine] 11875-093 500 ml
Antibiotics & Antimycotics
Antibiotic/Antimycotic (100x) 15240-062 100 ml
Fungizone antimycotic 15290-018 20 ml
Penicillin-Streptomycin (10,000 units/ml) 15140-122 100 ml
Geneticin selective antibiotic (G418 sulfate) 11811-023 1 g
Geneticin selective antibiotic (G418 sulfate) 11811-031 5 g
Balanced Salt Solutions
DPBS (10x) [without calcium, magnesium, phenol red] 14200-075 500 ml
DPBS (1x) [without calcium, magnesium, phenol red] 14190-144 500 ml
Dissociation Reagents
TrypLE™ Express stable trypsin-like enzyme [with phenol red] 12605-010 100 ml
Trypsin-EDTA (1x) [0.05% trypsin, 0.53 mM EDTA-4Na, with phenol red] 25300-054 100 ml
Trypsin-EDTA (10x) [0.5% trypsin, 5.3 mM EDTA-4Na] 15400-054 100 ml
Buffers & Supplements
HEPES Buffer (1M) 15630-080 100 ml
Sodium Bicarbonate (7.5%, w/v) 25080-094 100 ml
B-27 supplement (50x) 17504-044 10 ml
L-Glutamine (200 mM) (100x) 25030-081 100 ml
MEM Non-Essential Amino Acids (10 mM) (100x) 11140-050 100 ml
Sodium Pyruvate (100 mM) (100x) 11360-070 100 ml
Fetal Bovine Serum, Certified [origin: USA] 16000-044 500 ml
Fetal Bovine Serum, Certified [origin: USA] 16000-xxx 50 ml
Fetal Bovine Serum, Qualified [USDA approved sources] 10437-028 500 ml
Horse Serum [origin: New Zealand] 16050-122 500 ml
Newborn Calf Serum [origin: New Zealand] 16010-159 500 ml
Transfection Reagents
Lipofectamine 2000 11668-027 0.75 ml
Oligofectamine 12252-011 1 ml
Competent Cells
MAX Efficiency DH5α competent cells 18258-012 1 ml
Library Efficiency DH5α competent cells 18263-012 1 ml
Subcloning Efficiency DH5α competent cells 18265-017 2 ml
One Shot TOP10 chemically competent E. coli C4040-03 20 rxns
XL1-Blue Subcloning Grade competent cells (Stratagene) 200130 each
XL1-Blue competent cells (Stratagene) 200249 each
XL10-Gold competent cells (Stratagene) 200315 each
BL21-CodonPlus (DE3)-RIL competent cells (Stratagene) 230245 each
PCR Cloning
TOPO TA Cloning Kit (with PCR 2.1-TOPO vector) with One Shot TOP10 E. coli K4500-01 20 rxns