Products

Media, Cells and Reagents

The catalog of items available from the Tissue Culture Facility is available through iLab (https://uva.corefacilties.org). To see the catalog and purchase items, you need to have an account in iLab and have access to a PTAEO. See:
https://med.virginia.edu/core-facilities/ilab-billing-and-reservation-system-2/ilab-billing-and-reservation-system/
https://med.virginia.edu/core-facilities/ilab-billing-and-reservation-system-2/ilab-billing-and-reservation-system/ptaeo-in-ilab/

On the MolMart Catalog tab, you can search for products by name or catalog number. The UNSPSC Category search is not yet fully implemented so you may not some stocked items with a category search.

To purchase items, come to room 226 Pinn Hall  9 a.m. to 5 p.m. M-F.

  • Find media in cold cabinets, buffers and pipet tips in cabinets against wall nearest to hall.
  • check out items at a self service station, see instructions
  • Bills will be sent the next month.

The items in the Tissue Culture Facility are available only to researchers at the University of Virginia.

Media

The media below are supplied by Invitrogen

Description

Basal Media

Invitrogen Catalog
Number
Unit
DMEM (high glucose) [with 4500 mg/ml D-glucose, L-glutamine, without 11965-092
500 ml
DMEM (low glucose) [with 1000 mg/ml D-glucose, 110 mg/L sodium pyruvate, 11054-020
500 ml
DMEM (low glucose) [with 1000 mg/ml D-glucose, L- glutamine, 110 mg/L 11885-084
500 ml
DMEM/F-12 (1:1) [with 15 mM HEPES, L-glutamine]
11330-032
500 ml
DMEM/F-12 (1:1) [with L-glutamine, 15 mM HEPES, pyridoxine-HCl, without 11039-021
500 ml
DMEM/F-12 (1:1) [with L-glutamine, without HEPES]
11320-033
500 ml
F-12 Nutrient Mixture (Ham’s) [with L-glutamine]11765-054
500 ml
Grace’s Insect Medium, supplemented
11605-094
500 ml
IMDM [with L-glutamine, 25 mM HEPES, 3024 mg/L sodium 12440-046
1000 ml
IMDM [with L-glutamine, 25 mM HEPES, 3024 mg/L sodium 12440-053
500 ml
McCoy’s 5A Medium (modified) [with L-glutamine]16600-082
500 ml
Medium 199 [with Earle’s salts, L-glutamine, 2200 mg/L sodium 11150-059500 ml
MEM [with Earle’s salts, L-glutamine]
11095-080
500 ml
MEM [with Earle’s salts, non-essential amino acids, without L-glutamine]10370-021
500 ml
Opti-MEM I, reduced serum medium [with HEPES, 2400 mg/L sodium bicarbonate, hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace 31985-070
500 ml
RPMI Medium 1640 [with L-glutamine, without phenol red]11835-030
500 ml
RPMI Medium 1640 [with L-glutamine]
11875-093
500 ml

Antibiotics & Antimycotics

Description

Basal Media
Invitrogen Catalog
Number
Unit
Antibiotic/Antimycotic (100x)
15240-062
100 ml
Fungizone antimycotic
15290-018
20 ml
Penicillin-Streptomycin (10,000 units/ml)15140-122
100 ml
Geneticin selective antibiotic (G418 sulfate)
11811-023
1 g
Geneticin selective antibiotic (G418 sulfate)11811-031
5 g

Balanced Salt Solutions

Description

Basal Media
Invitrogen Catalog
Number
Unit
DPBS (10x) [without calcium, magnesium, phenol red]14200-075
500 ml
DPBS (1x) [without calcium, magnesium, phenol red]
14190-144
500 ml

Dissociation Reagents

Description

Basal Media

Invitrogen Catalog
Number
Unit
TrypLE™ Express stable trypsin-like enzyme [with phenol red}
12605-010
100 ml
Trypsin-EDTA (1x) [0.05% trypsin, 0.53 mM EDTA-4Na, with phenol red]25300-054
100 ml
Trypsin-EDTA (10x) [0.5% trypsin, 5.3 mM EDTA-4Na]15400-054
100 ml

Buffers & Supplements

Description

Basal Media
Invitrogen Catalog
Number
Unit
HEPES Buffer (1M)
15630-080
100 ml
Sodium Bicarbonate (7.5%, w/v)
25080-094
100 ml
B-27 supplement (50x)
17504-044
10 ml
L-Glutamine (200 mM) (100x)
25030-081
100 ml
MEM Non-Essential Amino Acids (10 mM) (100x)
11140-050
100 ml
Sodium Pyruvate (100 mM) (100x)
11360-070
100 ml

Sera

Description

Basal Media
Invitrogen Catalog
Number
Unit
Fetal Bovine Serum, Certified [origin: USA]16000-044

500 ml
Fetal Bovine Serum, Certified [origin: USA]16000-xxx
50 ml
aliquot
Fetal Bovine Serum, Qualified [USDA approved sources]10437-028
500 ml
Horse Serum [origin: New Zealand]
16050-122
500 ml
Newborn Calf Serum [origin: New Zealand]
16010-159
500 ml

Transfection Reagents

Description

Basal Media
Invitrogen Catalog
Number
Unit
Lipofectamine 2000
11668-027
0.75 ml
Oligofectamine
12252-011
1 ml

Competent Cells

Description

Basal Media
Invitrogen Catalog
Number
Unit
MAX Efficiency DH5α competent cells
18258-0121 ml
Library Efficiency DH5α competent cells
18263-012
1 ml
Subcloning Efficiency DH5α competent cells18265-017
2 ml
One Shot TOP10 chemically competent E. coliC4040-03
20 rxns
XL1-Blue Subcloning Grade competent cells (Stratagene)200130each
XL1-Blue competent cells (Stratagene)
200249
each
XL10-Gold competent cells (Stratagene)200315each
BL21-CodonPlus (DE3)-RIL competent cells (Stratagene)230245
each

PCR Cloning

Description

Basal Media
Invitrogen Catalog
Number
Unit
TOPO TA Cloning Kit (with PCR 2.1-TOPO vector) with One Shot TOP10 E. coliK4500-01
20 rxns