Equipment

Zeiss Lightsheet 7

Standalone sealed box system (laser safe) for multiview imaging of living and optically cleared specimens up to 2cm in size at subcellular resolution given any refractive index between 1.33 – 1.58
Laser lines: 405nm, 445nm, 488nm, 515nm, 561nm, 638nm
Objectives: 5x, 20x
Live cell imaging: includes sample chamber providing heating & cooling

Zeiss LSM 980 Confocal w/ AiryScan 2

Inverted spectral imaging confocal microscope equipped with AiryScan 2 which enables 2x higher resolution images at high speed acquisition, and an ensemble of 2 multi-alkaline PMTs, 32-channel spectral GaAsP, and 2 near-IR GaAs detectors
Equipped with Ai sample finder for automatically locating tissue section
Laser lines: 405nm, 458nm, 488nm, 514nm, 543nm, 594nm, 633nm, and 730nm
Objectives: 10x, 20x, 40x, and 63x
Long-term live cell imaging: includes a fully enclosed CO2 chamber

System Specifications

Operation Instructions

Zeiss LSM 880 Confocal w/ AiryScan

Inverted spectral imaging confocal microscope equipped with AiryScan/fastAiryScan which enables 1.7x higher resolution images at high speed acquisition
Equipped with a 34 channel spectral array
Laser lines: 405nm, 458nm, 488nm, 514nm, 543nm, 594nm and 633nm
Objectives: 10x, 20x, 40x, and 63x
Long-term live cell imaging: includes a fully enclosed CO2 chamber

Zeiss LSM 700 Confocal

Inverted microscope ideal for collecting transmitted light images as well as conventional confocal fluorescence images
Equipped with 4 channel detection
Laser lines: 405, 488, 543, 633 nm
Objectives: 10x, 20x, 40x, and 63x
Short-term live cell imaging: includes a CO2 incubator

Leica Thunder Imager 3D Cell Culture w/ TIRF

Fully automated inverted microscope equipped with a W-View Gemini for dual color TIRF as well as color and monochromatic cameras for widefield and brightfield, respectively
Laser lines for TIRF: 405, 488, 561, and 638nm
Objectives: 5x, 10x, 20x, 40x, 63x
Live cell imaging: includes CO2 connection with stage top incubation

Jeol JSM-IT-800HL FE-SEM

The integrated In-Lens Schottky Plus and low aberration condenser lens achieves high beam brightness. Ample probe current (100nA@5kV) is available even at low accelerating voltage.
Stable observation is possible with Neo Engine (New Electron Optical Engine) while adjusting various microscope parameters.
The hybrid lens (HL) lens are equipped with an electromagnetic/electrostatic field superposed objective lens developed from the versatile out-lens. The high resolution observation and analysis of a wide variety of specimens from metals to nano materials are possible.
Scintillator Backscattered Electron Detector (SBED)

Acquifer HIVE

WorkStation 1

Workstation 2

Analysis Software

The Core has licenses and service agreements for cutting-edge image analysis software.

Lightsheet Microscopy Sample Preparation

LifeCanvas SmartBatch+ Tissue Clearing and Immunolabeling
Active 3D tissue clearing (Clear+ method) and immunolabeling (eFLASH with stochastic electrotransport technologies) for intact tissue volumes
Duration: ~2 weeks w/ immunolabeling (endogenous FPs absent) or ~1 week w/o immunolabeling (endogenous FPs present)

Electron Microscopy Sample Preparation

Crest X-Light V3 Spinning Disk Confocal System

High-speed spinning disk confocal system built on a motorized Nikon Ti2-E platform, designed for fast, gentle live-cell imaging.

Crest X-Light V3 LFOV25 spinning disk with 50/250 µm switchable pinhole disk
7-line Celesta laser launch with despeckling for uniform illumination
Motorized Nikon Ti2-E stand with Perfect Focus System (PFS) and encoded motorized stage
Hamamatsu ORCA-FusionBT back-thinned sCMOS camera (2304 x 2304, up to 95% QE, 6.5 µm pixel, 31 fps at 16-bit)
NIS-Elements AR+6D acquisition software with wavelength switcher and filter wheel modules
Objective lineup: CFI60 Plan Apochromat Lambda D 10x, 20x, 40x, and 60x oil immersion (up to NA 1.42)
HP Z4 workstation (Xeon 6-core, 128GB RAM, NVIDIA RTX PRO 4000 GPU) with 31.5" 4K Thunderbolt display

Advanced Microscopy Facility