Equipment

The nCounter Pro Analysis System and the Sprint Profiler provide a cost-effective automated solution for multiplex analysis of up to 800 targets. The simple workflow requires just 15 minutes of hands-on time and produces highly reproducible data, requiring no amplification or technical replicates.

The panels cover different areas of research, such as Oncology, Immunology, Cardiovascular Diseases, Cell & Gene Therapy, Stem Cells, Regenerative Medicine, Infections Disease, Neuroscience, etc.

Customizing panels is another available tool. It is possible to include different targets in the chosen panel or customize your panel.

nCounter can be a shift option in studies that use the PCR technique, eliminating primer design and validation steps and optimizing the use of biological samples without technical replicates.

The nCounter technology requires no amplification or cDNA synthesis.

Analyzes can be performed using nSolver or Rosalinda software, both online platforms. Both software is easy to use, generating high throughput data and publishable pictures in less than 1 hour.

More information:

nCounter Overview

The Visium CytAssist is an instrument designed to transfer transcriptomic probes from standard glass slides to Visium slides.

Whole transcriptome spatial discovery in a single-cell scale.

Visium HD Spatial Gene Expression slides contain two 6.5 x 6.5 mm Capture Areas with a continuous lawn of oligonucleotides arrayed in ~11 million 2 x 2 μm barcoded squares without gaps, achieving single–cell–scale spatial resolution.

More information:

If you have an interest or want to know more about this platform, please contact Dr Ana de Oliveira at [email protected]

CosMxTM Spatial Molecular Imager (SMI) is the first high-plex in situ analysis platform to provide spatial multi-omics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution. CosMx SMI enables rapid quantification and visualization of 1,000 or 6,000 RNA and 64 or 120 proteins at the same time. It is the flexible, spatial single-cell imaging platform that will drive deeper insights for cell atlasing, tissue phenotyping, cell-cell interactions, cellular processes, and biomarker discovery.

The CosMxTM SMI platform is an integrated system with mature cyclic in situ hybridization chemistry, an ultra-high-resolution imaging readout instrument, and interactive data analysis and visualization software.

CosMx Same-Cell MultiOmics

Performing spatially-resolved multi-omics analysis on the same tissue section.

Run the RNA and Protein panels in the same tissue using CosMx platform. This workflow enable the detection of over 19,000, 6,000 or 1,000 RNA and 64 proteins (+ 8 custom protein) in the FFPE samples.

Applications:

Cell phenotyping
Tissue context (cells vs stroma structures)
Validate the candidate biomarkers directly in tissue by aligning protein detection with RNA expression at subcellular resolution

More information:

CosMx Overview

IsoCode Chip – Single-Cell Analysis

Isoplexis is a multiplex phenotyping analysis that allows complete functional characterization of a single-cell.

Cells are initially stained (2 markers) and captured individually in the chip’s microchambers. Over 30 cytokines, chemokines, and a full range of phosphoproteins are monitored for 20 hours.

Panel List:

Single-Cell Secretome

Human Adaptive Immune

Granzyme B, IFN-γ, MIP-1α, Perforin, TNF-α, TNF-β, GM-CSF, IL-2, IL-5, IL-7, IL-8, IL-9, IL-12, IL-15, IL-21, CCL11, IP-10, MIP-1β, RANTES, IL-4, IL-10, IL-13, IL-22, TGFβ1, sCD137, sCD40L, IL-1β, IL-6, IL-17A, IL-17F, MCP-1, MCP-4

Human Natural Killer

Granzyme B, IFN-γ, MIP-1α, Perforin, TNF-α, TNF-β, GM-CSF, IL-2, IL-5, IL-7, IL-8, IL-9, IL-12, IL-15, IL-21, CCL11, IP-10, MIP-1β, RANTES, IL-4, IL-10, IL-13, IL-22, TGFβ1, sCD137, sCD40L, IL-1β, IL-6, IL-17A, IL-17F, MCP-1, MCP-4

Human Innate Immune

IFN-γ, MIP-1α, TNF-α, TNF-β, GM-CSF, IL-8, IL-9, IL-15, IL-18, TGF-α, IL-5, CCL11, IP-10, MIP-1β, RANTES, BCA-1, IL-10, IL-13, IL-22, sCD40L, IL-1β, IL-6, IL-12-p40, IL-12, IL-17A, IL-17F, MCP-1, MCP-4, MIF, EGF, PDGF-BB, VEGF

Human Inflammation

GM-CSF, IFN-γ, IL-2, IL-12, TNF-α, TNF-β, IL-4, IL-5, IL-7, IL-9, IL-13, CCL11, IL-8, IP-10, MCP-1, MCP-4, MIP-1α, MIP-1β, RANTES, IL-10, IL-15, IL-22, TGF-β1, IL-1β, IL-6, IL-17A, IL-17F, IL-21, Granzyme B, Perforin, sCD40L, sCD137

Mouse Adaptive Immune

Granzyme B, IFN-γ, MIP-1α, TNF-α, GM-CSF, IL-2, IL-5, IL-7, IL-12p70, IL-15, IL-21, sCD137, CCL11, CXCL1, CXCL13, IP-10, RANTES, Fas, IL-4, IL-10, IL-13, IL-27, TGFβ1, IL-6, IL-17A, MCP-1, IL-1β

Single-Cell Signaling

Human Adaptive Immune

TNF-a, IFN-g, PERFORIN, GRANZYME B, IL-10, MIP-1b, IL-2, GM-CSF, P-IkBA, P-NF-kB p65, P-Stat3, P-MEK1/2, P-Stat1, P-Stat5, IL-8

CodePlex Chip - Secretome Analysis in Supernatant or Biological Fluid

Completely automated workflow

5.5 uL per sample in duplicate
Measure more than 30 cytokines in bulk
Run 8 samples per Chip
Modular, load 8-32 samples per run
Limit of detection: 5-5000 pg/ml

Panel List

Human Adaptive Immune

GM-CSF, Granzyme B, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-13, IL-15, IL-17A, IP-10, MCP-1, MIP-1α, MIP-1β, Perforin, sCD137, TNF-α, TNF-β

Human Innate Immune

EGF, GM-CSF, Granzyme B, IFN-γ, IL-1β, IL-4, IL-6, IL-7, IL-8, IL-10, IL-15, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-BB, sCD137, TNF-α, VEGF

Mouse Adaptive Immune

GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-17A, IP-10, KC, MCP-1, MIP-1α, RANTES, TNF-α

Mouse Innate Immune

IFN-γ, TNF-α, MIP-1α, IL-15, GM-CSF, IL-5, IL-10, IL-13, IL-6, IL-17A, MCP-1, IP-10, MIP-1β, EGF, PDGF-BB, MIF

Mouse Inflammation

IFN-γ, TNF-α, MIP-1α, IL-2, IL-5, IL-10, IL-13, IL-4, IL-6, IL-1β, IL-17A, IL-12, MCP-1, IP-10, KC, GM-CSF

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