Guidelines for the use and interpretation of assays for monitoring autophagy
4/21/2022
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our…
Recent Experimental Developments in Studying Passive Membrane Transport of Drug Molecules
4/20/2022
The ability to measure the passive membrane permeation of drug-like molecules is of fundamental biological and pharmaceutical importance. Of significance, passive diffusion across the cellular membranes plays an effective role…
Spatial regulation of endosomes in growing dendrites
4/20/2022
Many membrane proteins are highly enriched in either dendrites or axons. This non-uniform distribution is a critical feature of neuronal polarity and underlies neuronal function. The molecular mechanisms responsible for…
Lysosomes to the rescue: Anterograde axonal lysosome transport and neuronal proteostasis
4/20/2022
Niemann-Pick is a lysosomal storage disease caused by loss of the lysosomal cholesterol exporter NPC1 and leads to axon degeneration. Roney et al. report that immature autophagosomes accumulate in axons…
Dynamics and distribution of endosomes and lysosomes in dendrites
4/20/2022
All cells are filled with membrane-bound organelles which are responsible for the synthesis and transport as well as degradation of membrane proteins. The localization of these organelles inside cells is…
4/18/2022
The morphogenic process of convergent thickening (CT) was originally described as the mediolateral convergence and radial thickening of the explanted ventral involuting marginal zone (IMZ) of Xenopus gastrulae (Keller and Danilchik 1988).…
Drug Combinations as a First Line of Defense against Coronaviruses and Other Emerging Viruses
12/22/2021
The world was unprepared for coronavirus disease 2019 (COVID-19) and remains ill-equipped for future pandemics. While unprecedented strides have been made developing vaccines and treatments for COVID-19, there remains a…
Cytokinetic Abscission Regulation in Neural Stem Cells and Tissue Development
8/27/2021
Purpose of Review How stem cells balance proliferation with differentiation, giving rise to specific daughter cells during development to build an embryo or tissue, remains an open question. Here, we…
Cep55: abscission boss or assistant?
8/27/2021
Abscission is the second stage of cytokinesis. Cep55, a coiled-coil protein, is thought to recruit endosomal sorting complexes required for transport (ESCRTs) to the midbody to complete abscission. However, recent…
Mechanical stretch scales centriole number to apical area via Piezo1 in multiciliated cells
7/2/2021
How cells count and regulate organelle number is a fundamental question in cell biology. For example, most cells restrict centrioles to two in number and assemble one cilium; however, multiciliated…