Flow Cytometry Core Facility resource description

The Flow Cytometry Core Facility provides all investigators at the University of Virginia access to high quality, cost effective flow cytometry services. In addition to providing access to a variety of instrumentation, the FCCF also provides scientific expertise necessary to effectively use this technology to enhance the scope and quality of scientific research performed at the University.

The state of the art facility occupies approximately 1800 sq. ft. and houses a variety from basic to high end flow cytometry instrumentation. A CyTOF 2 with Helios upgrade and Mass Cytometry Antibody bank provide advanced mass cytometry analysis. Cell sorting instruments are a 4-laser, 10-color FACSVantage SE TurboSort DIVA Cell Sorter, a 5-laser, 15-color BD FACSAria Fusion Cell Sorter that both provide 4-way high-speed cell sorting and a 5-laser, 15-color BD Influx Cell Sorter that provides up to 6-way high speed cell sorting services. Analytical instruments include two 2-laser 4-color FACSCalibur benchtop analyzers as well as a 3-laser 5-color and a 4-laser 10-color Cytek Upgraded FACSCalibur analyzer and a 4 laser 14 color Attune NxT with 96-well autosampler. Other instruments available include a three laser 9-color CyAn ADP LX benchtop cytometer, a four laser LSRFortessa with 17 fluorescent detectors, an ImageStreamX imaging flow cytometer and two Luminex Magpix bead-based multiplex analyzers. A multi-terabyte server is available for data storage as well as site licenses for data analysis software.

Researchers have the option, once trained, of performing their own analysis or utilizing the expertise of the facility’s staff to run their samples for them. Specialized training classes are offered for those researchers who wish to better understand the principles and techniques employed in this technology and prefer to directly acquire and/or analyze their own samples.