The Molecular Electron Microscopy Core (MEMC) is a state-of-the-art facility dedicated to high-resolution electron cryomicroscopy and electron cryotomography. It houses three electron microscopes, a 120kV Spirit, a 200kV F20, and a 300kV Titan Krios. These microscopes are available to researchers either for direct use, or aided by MEMC personnel, to collect data aimed at high-resolution structural biology projects.
By examining frozen, unstained samples in thse high resolution transmission electron microscopes and taking images from different angles, researchers get structural data down to the molecular level, without having to crystallize proteins. This data complements the expertise in protein X-ray crystallography and NMR in the Department of Molecular Physiology and Biophysics
The goal of the UVA SOM Shared Research Resource Cores is to provide researchers with access to state-of-the-art instrumentation, technology and scientific expertise for the communal advantage of our institution’s investigators, so that they can effectively carry out the research mission of the University. The MEMC supports this by providing access to the best microscopes available.
Please acknowledge use of the core with the following statement or similar: “The EM work was conducted at the Molecular Electron Microscopy Core facility at the University of Virginia, which is supported by the School of Medicine and built with NIH grant (G20-RR31199).”
Users of the Titan Krios must acknowledge its use and funding by NIH SIG grant S10-RR025067 and also use of the Falcon II direct detector, NIH SIG S10-OD018149.
The MEMC facility is located in the Life Sciences Annex on the Fontaine Research Campus.