The goal of the UVA SOM Shared Research Resource Cores is to provide researchers with access to state-of-the-art instrumentation, technology and scientific expertise for the communal advantage of our institution’s investigators, so that they can effectively carry out the research mission of the University. The MEMC supports this by providing access to the best microscopes available.
By examining frozen, unstained samples in these high resolution transmission electron microscopes and taking images from different angles, researchers get structural data down to the molecular level, without having to crystallize proteins. This data complements the expertise in protein X-ray crystallography and NMR in the Department of Molecular Physiology and Biophysics
Acheson, J. F., Ho, R., Goularte, N. F., Cegelski, L., and Zimmer, J. Molecular organization of the E. coli cellulose synthase macrocomplex. Nat Struct Mol Biol. 28(3):310-318, Mar 2021.
Zheng, W., Peña, A., Ilangovan, A., Clark, J. N.-B., Frankel, G., Egelman, E. H., and Costa, T. R. D. Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament. Proc Natl Acad Sci USA. 118(2):e2022826118, Jan 2021.
Wang, F., Gnewou, O., Modlin, C., Beltran, L. C., Xu, C., Su, Z., Juneja, P., Grigoryan, G., Egelman, E. H., and Conticello, V. P. Structural analysis of cross α-helical nanotubes provides insight into the designability of filamentous peptide nanomaterials. Nat. Commun. 12(1):407, Jan 2021.
Ward, A. E., Kiessling, V., Pornillos, O., White, J. M., Ganser-Pornillos, B. K., and Tamm, L. K. HIV-cell membrane fusion intermediates are restricted by Serincs as revealed by cryo-electron and TIRF microscopy. J Biol Chem. 295(45):15183-15195, Nov 2020.
Christensen, D. E., Ganser-Pornillos, B. K., Johnson, J. S., Pornillos, O., and Sundquist, W. I. Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro. Science. 370(6513):eabc8420, Oct 2020.