Basic Science Research Program – Scott Hollenbeck, MD

Characterizing the Lipedema Adipose Tissue Microenvironment: Focusing on Inflammation and Stem Cells

Lipedema is a chronic, progressive disorder characterized by the abnormal accumulation of fat, typically in the lower limbs, and is often associated with pain, swelling, and bruising. Unlike obesity, Lipedema is driven by complex molecular and cellular mechanisms, which are not yet fully understood. The adipose tissue microenvironment plays a central role in the development and progression of lipedema, with inflammation, angiogenesis, stem cells, and hormonal factors all contributing to the disease’s unique pathophysiology.
The laboratory focuses on two main aspects of lipedema research: (1) Understanding the role of inflammation and the signaling pathways that drive the progression of the disease, and (2) investigating the role of stem cells as key drivers of lipedema development. The research will explore how chronic inflammation in the adipose tissue microenvironment leads to alterations in blood vessel structure and function, contributing to swelling, fluid buildup, and vascular dysfunction. Additionally, by examining the interactions between stem cells, hormones, and inflammation, the laboratory aims to uncover key factors that influence disease progression. Advanced molecular techniques such as single-cell RNA sequencing, immunohistochemistry, and gene and protein expression profiling allow for detailed analysis of the tissue microenvironment at both the cellular and molecular levels. These techniques enable the identification of specific inflammatory pathways and stem cell interactions that may drive disease progression.

The laboratory collaborates with plastic surgery clinics and pioneers in the field, which helps pave the way for identifying biomarkers for lipedema, bridging the gap between scientific discovery and clinical application to improve patient care.