Binyong Liang, Ph.D.

Binyong Liang, Ph.D.
Assistant Professor
Email: bl9m@Virginia.EDU

Binyong Liang is from Shaoxing, China. He has been at UVA since 1998 and received his PhD in Chemistry from UVA in 2002.

Publications:

Kreutzberger AJB, Kiessling V, Liang B, Seelheim P, Jakhanwal S, Jahn R, Castle JD, Tamm LK. Reconstitution of calcium-mediated exocytosis of dense-core vesicles. Science Advances 19 Jul 2017: Vol. 3. No. 7. E1603208 DOI:10.1126/sciadv.1603208 (http://advances.sciencemag.org/content/3/7/e1603208.full)

Zdanowicz R, Kreutzberger A, Liang B, Kiessling V, Tamm LK, Cafiso DS. Complexin Binding to Membranes and Acceptor t-SNAREs Explains Its Clamping Effect on Fusion. Biophys J. 2017 Apr 26. pii: S0006-3495(17)30389-2. doi: 10.1016/j.bpj.2017.04.002. [Epub ahead of print]

Kiessling V, Liang B, Kreutzberger AJ, Tamm LK. Planar Supported Membranes with Mobile SNARE Proteins and Quantitative Fluorescence Microscopy Assays to Study Synaptic Vesicle Fusion. Front Mol Neurosci. 2017 Mar 16;10:72. doi: 10.3389/fnmol.2017.00072. eCollection 2017. (https://www.ncbi.nlm.nih.gov/pubmed/28360838)

Kucharska I, Liang B, Ursini N, Tamm LK. (2016) Molecular Interactions of Lipopolysaccharide with an Outer Membrane Protein from Pseudomonas aeruginosa Probed by Solution NMR. Biochemistry. 2016 Sep 13;55(36):5061-72. (https://www.ncbi.nlm.nih.gov/pubmed/27532487)

Liang B, Tamm LK. NMR as a tool to investigate the structure, dynamics and function of membrane proteins. Nat Struct Mol Biol. 2016 Jun 7;23(6):468-74. doi: 10.1038/nsmb.3226. (http://www.ncbi.nlm.nih.gov/pubmed/27273629)

Kreutzberger AJ, Liang B, Kiessling V, Tamm LK. Assembly and Comparison of Plasma Membrane SNARE Acceptor Complexes.  Biophys J. 2016 May 24;110(10):2147-50. doi: 10.1016/j.bpj.2016.04.011. Epub 2016 May 10. ( http://www.ncbi.nlm.nih.gov/pubmed/27178662)

Kiessling V, Liang B, Tamm LK. Reconstituting SNARE-mediated membrane fusion at the single liposome level. Methods Cell Biol. 2015;128:339-63. doi: 10.1016/bs.mcb.2015.02.005. Epub 2015 Apr 8. ( http://www.ncbi.nlm.nih.gov/pubmed/25997356)

Tamm LK, Lee J, Liang B. Capturing glimpses of an elusive HIV gp41 prehairpin fusion intermediate. Structure. 2014 Sep 2;22(9):1225-6. doi: 10.1016/j.str.2014.08.010. (http://www.ncbi.nlm.nih.gov/pubmed/25185826)

Kucharska, I.,  Seelheim, P., Edrington, T.,  Liang,  B., Tamm, L.K.  OprG Harnesses the Dynamics of its Extracellular Loops to Transport Small Amino Acids across the Outer Membrane of Pseudomonas aeruginosa  Structure December, 2015, 23:2234-2245. (http://www.ncbi.nlm.nih.gov/pubmed/26655471)

Kucharska, I., Edrington, T., Liang, B., Tamm, L.K.  Optimizing nanodiscs and bicelles for solution NMR studies of two Beta-barrel membrane proteins  J Biomol NMR  February, 2015, 61:261-274. (http://www.ncbi.nlm.nih.gov/pubmed/25869397)

Liang, B., Dawidowski, D., Ellena, J.F., Tamm, L.K., Cafiso, D.S. The SNARE motif of synaptobrevin exhibits an aqueous-interfacial partitioning that is modulated by membrane curvature. Biochemistry. Mar 11;53(9): 1945-94 (2014).  (http://www.ncbi.nlm.nih.gov/pubmed/24552121)

Liang, B., Kiessling, V., Tamm, L.K.  Prefusion structure of syntaxin-1A suggests pathway for folding into neuronal trans-SNARE complex fusion intermediate. Proc Natl Acad Sci USA, Nov 26; 110 (48):19348-9 (2013). (http://www.ncbi.nlm.nih.gov/pubmed/24218570)

Gregory, S.M., Harada, E., Liang, B., Delos, S.E., White, J.M., and Tamm, L.K. Structure and Function of the Complete Internal Fusion Loop From Ebolavirus Glycoprotein 2. Proc. Natl. Acad. Sci. U.S.A. 108: 11211-11216 (2011).  (http://www.ncbi.nlm.nih.gov/pubmed/21690393)

Ellena, J.F., Liang, B., Wiktorb, M., Stein, A., Cafiso, D.S., Jahn, R., Tamm, L.K. . Dynamic structure of lipid-bound synaptobrevin suggests a nucleation-propagation mechanism for trans-SNARE complex formation. PNAS 106(48):20306-11 (2009).(http://www.ncbi.nlm.nih.gov/pubmed/19918058)

Liang, B., Arora, A., Tamm, L.K. Fast-time scale dynamics of Outer membrane protein A by extended model-free analysis of NMR relaxation data.  Biochim. Biophys. Acta , 1798, 68-76. (2010). (http://www.ncbi.nlm.nih.gov/pubmed/19665446)

Liang, B. and Tamm, L.K.  Structure of outer membrane protein G by solution NMR spectroscopy. PNAS.104, 41:16140-16145 (2007). (http://www.ncbi.nlm.nih.gov/pubmed/17911261)

Tamm, L.K., Liang, B.   NMR of membrane proteins in solution. Prog. in  Nuc Mag Res Spec.  Vol.48 201-210 (2006).

Cierpicki, T., Liang, B., Tamm, L.K., Bushweller, J.H..  Increasing the Accuracy of Solution NMR Structures of Membrane Proteins by Application of Residual Dipolar Coupling. High-Resolution Structure of Outer Membrane Protein A.. J. Am Chem. Soc  128, 21:6947-6951 (2006). (http://www.ncbi.nlm.nih.gov/pubmed/16719475).

Liang, B., Bushweller, J.H., Tamm, L.K. Site-Directed Parallel Spin-Labeling and Paramagnetic Relaxation Enhancement in Structure Determination of Membrane Proteins by Solution NMR Spectroscopy. J. Am Chem. Soc  128,13:4389-4397 (2006). (http://www.ncbi.nlm.nih.gov/pubmed/16569016).

Tamm, L.K., Hong, H.and Liang, B.  Folding and assembly of beta-barrel membrane proteins. (Review)Biochim. Biophys. Acta. 1666: 250-263 (2004). (http://www.ncbi.nlm.nih.gov/pubmed/15519319).

Liang, B., L. Andrews, J. Li, and B.E. Bursten  Noble gas-actinide compounds: Evidence for the formation of distinct CUO(Ar)4-n(Xe)n and CUO(Ar)4-n(Kr)n (n = 1, 2, 3, 4) complexes. J. Am. Chem. Soc. 124: 9016 (2002).

Liang, B.and L. Andrews (2002). Infrared spectra and density functional theory calculations of group 6 transition metal sulfides in solid argon. J. Phys. Chem. A. 106: 6945.

Liang, B. and L. Andrews (2002). Infrared spectra and density functional theory calculations of group 4 transition metal sulfides. J. Phys. Chem. A. 106: 6295.

Liang, B., L. Andrews, J. Li, and B.E. Bursten (2002). Experimental and theoretical studies of the products of laser-ablated thorium atom reactions with H2O in excess argon. J. Am. Chem. Soc. 124: 6723.

Liang, B., L. Andrews, N. Ismail, and C.J. Marsden (2002). The first infrared spectra and quasirelativistic DFT studies of the US, US2, and US3 molecules. Inorg. Chem. 41: 2811.

Liang, B. and L. Andrews (2002). Reactions of laser-ablated Os, Ru atoms with carbon dioxide: Matrix infrared spectra and density functional calculations on OMCO, O2MCO, OMCO (M = Os, Ru), O2Os(CO)2 and OCRu(O2)CO. J. Phys. Chem. A. 106: 4042.

Liang, B. and L. Andrews (2002). Matrix infrared spectra and quasirelativistic DFT Studies of ThS and ThS2. J. Phys. Chem. A. 106: 4038.

Liang, B. and L. Andrews (2002). Infrared Spectra and Density Functional Theory Calculations of Group V Transition Metal Sulfides. J. Phys. Chem. A. 106: 3738.

Li, J., B.E. Bursten, B. Liang, B., L. Andrews (2002). Noble Gas-Actinide Compounds: Complexation of the CUO Molecule by Ar, Kr, and Xe Atoms in Noble-Gas Matrices. Science. 295: 2242.

Liang, B. and L. Andrews (2002). Reactions of laser-ablated rhenium atoms with carbon dioxide: Matrix infrared spectra and density functional calculations on OReCO, O2ReCO, ORe(CO)2, O2Re(CO)2, OReCOand ORe(CO)2. J. Phys. Chem. A. 106: 595.

Andrews, L., X. Wang, M.F. Zhou, and B. Liang (2002). Infrared spectrum of the hyponitrite dianion, N2O22-, isolated and insulated from stabilizing metal cations in solid neon. J. Phys. Chem. A. 106: 92.

Liang, B. and L. Andrew (2001). Infrared spectra of cis- and trans-peroxynitrite anion, OONO, in solid argon.J. Am. Chem. Soc. 123: 9848.

Andrews, L. and B. Liang (2001). Infrared spectrum of the hyponitrite dianion, N2O22-, isolated and insulated from stabilizing metal cations in solid argon. J. Am. Chem. Soc. 123: 1997.

Andrews, L., B. Liang, J. Li, and B.E. Bursten (2000). Ground-state reversal by matrix interaction: Electronic states and vibrational frequencies of CUO in solid argon and neon. Angew. Chem. Int. Edit. 39: 4565.

Andrews, L., M.F. Zhou, B. Liang, B., J. Li, and B.E. Bursten (2000). Reactions of laser-ablated U and Th with CO2: Neon matrix infrared spectra and density functional calculations of OUCO, OThCO, and other products.J. Am. Chem. Soc. 122: 11440.

Liang, B. and L. Andrews (2000). Reactions of laser-ablated Ag and Au atoms with carbon monoxide: Matrix infrared spectra and density functional calculations on Ag(CO)n (n = 2, 3), Au(CO)n (n = 1, 2) and M(CO)n+ (n = 1-4; M = Ag, Au). J. Phys. Chem. A. 104: 9156.

Kushto, G. P., F. Ding, B. Liang, X. Wang, A. Citra, and L. Andrews (2000). Infrared spectra of BeNO and MgNO in solid argon. Chem. Phys. 257: 223.

Liang, B., M.F. Zhou, and L. Andrews (2000). Reactions of laser-ablated Ni, Pd, and Pt atoms with carbon monoxide: Matrix infrared spectra and density functional calculations on M(CO)n (n = 1-4), M(CO)n (n = 1-3), and M(CO)n+ (n = 1-2), (M = Ni, Pd, Pt). J. Phys. Chem. 104: 3905.

Zhou, M. F., A. Citra, B. Liang, and L. Andrews (2000). Infrared spectra and density functional calculations of MO2, MO3, (O2)MO2, MO4, MO2 (M = Re, Ru, Os) and ReO3, ReO4 in solid neon and argon. J. Phys. Chem. A. 104: 3457.

Andrews, L., P.F. Souter, W.D. Bare, and B. Liang (1999). Reactions of laser-ablated Mo and W atoms with dinitrogen: Infrared spectra of metal nitrides, dinitrides, and complexes in solid argon and nitrogen. J. Phys. Chem. A. 103: 4649.
Zhou, M. F., B. Liang, and L. Andrews (1999). Infrared spectra of OMCO (M = Cr-Ni), OMCO (M = Cr-Cu), and MCO2 (M = Co-Cu) in solid argon. J. Phys. Chem. A. 103: 2013.