Volker Kiessling, Ph.D.

May 11, 2016 by klm3b@virginia.edu

Volker Kiessling, Ph.D.
Assistant Professor
Email: vgk3c@Virginia.EDU

Volker Kiessling is from Kirchheim am Neckar, Germany. He earned his PhD at Technical University, Munich, in 1999 and came to the University of Virginia in 2000.





Kreutzberger AJB, Kiessling V, Stroupe C, Liang B, Preobraschenski J, Ganzella M, Kreutzberger MAB, Nakamoto R, Jahn R, Castle JD, Tamm LK. In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties. Nat Commun. 2019 Aug 29;10(1):3904. doi: 10.1038/s41467-019-11873-8. (https://www.ncbi.nlm.nih.gov/pubmed/31467284)

Kreutzberger AJB, Kiessling V, Liang B, Yang ST, Castle JD, Tamm LK. Asymmetric Phosphatidylethanolamine Distribution Controls Fusion Pore Lifetime and Probability. Biophys J. 2017 Oct 13. pii: S0006-3495(17)31026-3. doi: 10.1016/j.bpj.2017.09.014. [Epub ahead of print](https://www.ncbi.nlm.nih.gov/pubmed/29037600)

Kreutzberger AJB, Kiessling V, Liang B, Seelheim P, Jakhanwal S, Jahn R, Castle JD, Tamm LK. Reconstitution of calcium-mediated exocytosis of dense-core vesicles. Science Advances 19 Jul 2017: Vol. 3. No. 7. E1603208 DOI:10.1126/sciadv.1603208 (http://advances.sciencemag.org/content/3/7/e1603208.full)

Yang ST, Kreutzberger AJB, Kiessling V, Ganser-Pornillos BK, White JM, Tamm LK. (2017) HIV virions sense plasma membrane heterogeneity for cell entry. Sci. Adv. Jun 2017: Vol. 3, no. 6, e1700338(http://advances.sciencemag.org/content/3/6/e1700338)

Zdanowicz R, Kreutzberger A, Liang B, Kiessling V, Tamm LK, Cafiso DS. Complexin Binding to Membranes and Acceptor t-SNAREs Explains Its Clamping Effect on Fusion. Biophys J. 2017 Apr 26. pii: S0006-3495(17)30389-2. doi: 10.1016/j.bpj.2017.04.002. [Epub ahead of print]

Kiessling V, Liang B, Kreutzberger AJ, Tamm LK. Planar Supported Membranes with Mobile SNARE Proteins and Quantitative Fluorescence Microscopy Assays to Study Synaptic Vesicle Fusion. Front Mol Neurosci. 2017 Mar 16;10:72. doi: 10.3389/fnmol.2017.00072. eCollection 2017. (https://www.ncbi.nlm.nih.gov/pubmed/28360838)

Kreutzberger AJ, Liang B, Kiessling V, Tamm LK. Assembly and Comparison of Plasma Membrane SNARE Acceptor Complexes.  Biophys J. (2016) May 24;110(10):2147-50. (http://www.ncbi.nlm.nih.gov/pubmed/27178662)

Yang ST, Kreutzberger AJ, Lee J, Kiessling V, Tamm LK. The role of cholesterol in membrane fusion. Chem Phys Lipids. (2016) May 11. pii: S0009-3084(16)30055-X. (http://www.ncbi.nlm.nih.gov/pubmed/27179407)

Yang ST, Kiessling V, Tamm LK.  Line tension at lipid phase boundaries as driving force for HIV fusion peptide-mediated fusion. Nat Commun. (2016) Apr 26;7:11401. (http://www.ncbi.nlm.nih.gov/pubmed/27113279)

Kreutzberger AJ, Kiessling V, Tamm LK High Cholesterol Obviates a Prolonged Hemifusion Intermediate in Fast SNARE-Mediated Membrane Fusion. Biophys J. (2015) Jul 21;109(2):319-29. (http://www.ncbi.nlm.nih.gov/pubmed/26200867)

Kiessling V, Liang B, Tamm LK. Reconstituting SNARE-mediated membrane fusion at the single liposome level. Methods Cell Biol. (2015) 128:339-63.

Hernandez, J.M., Kreutzberger, A.J., Kiessling, V., Tamm, L.K., Jahn, R. Variable cooperativity in SNARE-mediated membrane fusion. Proc Natl Acad Sci USA. Aug 19;111(33);12037-42 (2014). (http://www.ncbi.nlm.nih.gov/pubmed/25092301)

Lu, B., Kiessling, V., Tamm, L.K., Cafiso, D.S.  The Juxtamembrane Linker of Full-length Synaptotagmin 1 Controls Oligomerization and Calcium-dependent Membrane Binding. J Biol Chem.  Aug 8;289(32):22161-71 (2014). (http://www.ncbi.nlm.nih.gov/pubmed/24973220)

Moissoglu, K., Kiessling, V., Wan, C., Hoffman, B.D., Norambuena, A., Tamm, L.K., Schwartz, M.A. Regulation of Rac Translocation and Activation by Membrane Domains and Their Boundaries. Journal of Cell Science. (2014) (In Press). (http://www.ncbi.nlm.nih.gov/pubmed/24695858)

Liang, B., Kiessling, V., Tamm, L.K.,  Prefusion structure of syntaxin-1A suggests pathway for folding into neuronal trans-SNARE complex fusion intermediate. Proc Natl Acad Sci USA. Nov 26; 110 (48):19348-9 (2013).(http://www.ncbi.nlm.nih.gov/pubmed/24218570)

Kiessling, V.,  Ahmed, S.,  Domanska, M.,  Holt, M.,  Jahn, R., Tamm, L.K.  Rapid Fusion of Synaptic Vesicles with Reconstituted Target SNARE Membranes. Biophysical Journal 104:1950-1958 (2013).(http://www.ncbi.nlm.nih.gov/pubmed/23663838)

Kiessling, V. Transbilayer coupling of lipid dynamics. Biophys J. Dec. 19;103(12):2409-10 (2012). (http://www.ncbi.nlm.nih.gov/pubmed/23260042)

Wan, C., Kiessling, V., Cafiso, D.S., & Tamm, L.K. Partitioning of Synaptotagamin 1 C2 Domains between Liquid-Ordered and Liquid-Disordered Inner Leaflet Lipid Phases. Biochemistry 50: 2478-2485 (2011). (http://www.ncbi.nlm.nih.gov/pubmed/21322640)

Arouri, A., Kiessling, V.,  Tamm, L.K., Dathe, M. & Blume, A. Morphological changes induced by the action of antimicrobial peptides on supported lipid bilayers. Journal of Physical Chemistry B 115: 158-167 (2011). (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033229)

Kiessling, V., Domanska, M.K. & Tamm, L.K.  Single SNARE-mediated vesicle fusion observed in vitro by polarized TIRFM. Biophysical Journal 99: 4047-4055 (2010). (http://www.ncbi.nlm.nih.gov/pubmed/21156148)

Domanska, M.K., Kiessling, V. & Tamm, L.K. Docking and fast fusion of synaptobrevin vesicles depends on the lipid compositions of the vesicle and the acceptor SNARE complex-containing target membrane. Biophysical Journal 99: 2936-2946 (2010). (http://www.ncbi.nlm.nih.gov/pubmed/21044591)

Domanska, M.K., Kiessling, V., Stein, A., Fasshauer, D. & Tamm, L.T.  Single Vesicle Millisecond Fusion Kinetics Reveals Number of SNARE Complexes Optimal for Fast SNARE-mediated Membrane Fusion. Journal of Biological Chemistry. 284(46): 32158-32166 (2009). (http://www.ncbi.nlm.nih.gov/pubmed/19759010)

Murray, D.H., Tamm, L.K., Kiessling, V.  Supported Double Membranes. J. Structural Biology 168, 183-189 (2009). (http://www.ncbi.nlm.nih.gov/pubmed/19236921)

Kiessling, V., Wan, C., & Tamm, L.K. Domain coupling in asymmetric lipid bilayers. Biochimica et Biophysica Acta, 1788: 64-71 (2009). (http://www.ncbi.nlm.nih.gov/pubmed/18848518)

Wan, C., Kiessling, V., & Tamm, L.K. Coupling of Cholesterol-Rich Lipid Phases in Assymmetric Bilayers. Biochemistry 47: 2190-2198 (2008). (http://www.ncbi.nlm.nih.gov/pubmed/18215072)

Kiessling, V., Domanska, M.K., Murray, D., Wan, C., and Tamm L.K. Supported Lipid Bilayers: Development and Application in Chemical Biology.  Wiley Encyclopedia of Chemical Biology, John Wiley & Sons, Hoboken, Volume 4, pp. 411-422 (2008). (Full text in PDF)

Kiessling V., Crane, J.M., Tamm L.K.  Transbilayer Effects of Raft-Like Lipid Domains in Asymmetric Planar Bilayer Measured by Single Molecule Tracking.  Biophys J. 91: 3313-3326 (2006). Erratum in: Biophys J. Jan 15;92(2):698 (2007). (http://www.ncbi.nlm.nih.gov/pubmed/16905614) (Erratum)

Kiessling, V. Imaging Fast SNARE Mediated-Membrane Fusion in Planar-Supported Bilayers. Biophysical Journal. 89: 2185-2186 (2005). (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1366721/)

Kiessling, V., Vassanelli, S.  Potassium Channel Gating in Adhesion: from an Oocyte-Silicon to a Neuron-Astrocyte Adhesion Contact. Eur Biophys J. 34: 113-126 (2005). (http://www.ncbi.nlm.nih.gov/pubmed/15776285)

Crane, J.M., Kiessling, V. & Tamm, L.K.  Measuring Lipid Asymmetry In Planar Supported Bilayers by Fluorescence Interference Contrast Micorscopy. Langmuir. 21: 1377-1388 (2005). (http://www.ncbi.nlm.nih.gov/pubmed/15697284)

Tamm, L.K., Crane, J. & Kiessling, V. Membrane fusion: a structural perspective on the interplay of lipids and proteins. (Review) Curr. Op. Struct. Biol. 13: 453-466 (2003). (http://www.ncbi.nlm.nih.gov/pubmed/12948775)

Kiessling, V., Tamm, L.K.  Measuring distances in supported bilayers by fluorescence interference-contrast microscopy: polymer supports and SNARE proteins. Biophys. J. 84: 408-418 (2003). (http://www.ncbi.nlm.nih.gov/pubmed/12524294)

Tamm, L.K., Kiessling, V. & Wagner, M.L. Membrane Dynamics. Encyclopedia of Life Sciences. Nature Publishing Group, Macmillan Publishers, Ltd. (2001). (http://www.els.net)

Kiessling, V., Müller, B., Fromherz, P.  Extracellular Resistance in Cell Adhesion measured with a Transistor Probe. Langmuir. 16(7): 3517-3521 (2000).

Fromherz, P., Kiessling, V., Kottig, K., Zeck, G. Membrane transistor with giant lipid vesicle touching a silicon chip. Applied Physics A. 69(5): 571-576 (1999).