Volker Kiessling, Ph.D.

May 11, 2016 by

Volker Kiessling is from Kirchheim am Neckar, Germany. He earned his PhD at Technical University, Munich, in 1999 and came to the University of Virginia in 2000.


Drunken lipid membranes, not drunken SNARE proteins, promote fusion in a model of neurotransmitter release. Coffman RE, Kraichely KN, Kreutzberger AJB, Kiessling V, Tamm LK, Woodbury DJ.Front Mol Neurosci. 2022 Oct 14;15:1022756. doi: 10.3389/fnmol.2022.1022756. eCollection 2022.PMID: 36311016 (

SARS-CoV-2 requires acidic pH to infect cells. Kreutzberger AJB, Sanyal A, Saminathan A, Bloyet LM, Stumpf S, Liu Z, Ojha R, Patjas MT, Geneid A, Scanavachi G, Doyle CA, Somerville E, Correia RBDC, Di Caprio G, Toppila-Salmi S, Mäkitie A, Kiessling V, Vapalahti O, Whelan SPJ, Balistreri G, Kirchhausen T.Proc Natl Acad Sci U S A. 2022 Sep 20;119(38):e2209514119. doi: 10.1073/pnas.2209514119. Epub 2022 Sep 1.PMID: 36048924 (

Complexin-1 and synaptotagmin-1 compete for binding sites on membranes containing PtdInsP2. Liang Q, Ofosuhene AP, Kiessling V, Liang B, Kreutzberger AJB, Tamm LK, Cafiso DS.Biophys J. 2022 Sep 20;121(18):3370-3380. doi: 10.1016/j.bpj.2022.08.023. Epub 2022 Aug 25.PMID: 36016497 (

Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments. Kreutzberger MAB, Sobe RC, Sauder AB, Chatterjee S, Peña A, Wang F, Giron JA, Kiessling V, Costa TRD, Conticello VP, Frankel G, Kendall MM, Scharf BE, Egelman EH.Nat Commun. 2022 Mar 17;13(1):1422. doi: 10.1038/s41467-022-29069-y.PMID: 35301306 ( )

Endosomes supporting fusion mediated by vesicular stomatitis virus glycoprotein have distinctive motion and acidification. Cabot M, Kiessling V, White JM, Tamm LK.Traffic. 2022 Apr;23(4):221-234. doi: 10.1111/tra.12836. Epub 2022 Feb 21.PMID: 35147273 ( )

Conserved arginine residues in synaptotagmin 1 regulate fusion pore expansion through membrane contact. Nyenhuis SB, Karandikar N, Kiessling V, Kreutzberger AJB, Thapa A, Liang B, Tamm LK, Cafiso DS.Nat Commun. 2021 Feb 3;12(1):761. doi: 10.1038/s41467-021-21090-x.PMID: 33536412 (

Ebola virus glycoprotein interacts with cholesterol to enhance membrane fusion and cell entry. Lee J, Kreutzberger AJB, Odongo L, Nelson EA, Nyenhuis DA, Kiessling V, Liang B, Cafiso DS, White JM, Tamm LK.Nat Struct Mol Biol. 2021 Feb;28(2):181-189. doi: 10.1038/s41594-020-00548-4. Epub 2021 Jan 18.PMID: 33462517 ( )

ATP and large signaling metabolites flux through caspase-activated Pannexin 1 channels. Narahari AK, Kreutzberger AJ, Gaete PS, Chiu YH, Leonhardt SA, Medina CB, Jin X, Oleniacz PW, Kiessling V, Barrett PQ, Ravichandran KS, Yeager M, Contreras JE, Tamm LK, Bayliss DA.Elife. 2021 Jan 7;10:e64787. doi: 10.7554/eLife.64787.PMID: 33410749 ( )

Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2. Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, Kattan I, Harris M, Liang B, Kenworthy AK, Desai BN, Leitinger N, Anantharam A, Castle JD, Tamm LK.Elife. 2020 Nov 9;9:e62506. doi: 10.7554/eLife.62506.PMID: 33164744 (

HIV-cell membrane fusion intermediates are restricted by Serincs as revealed by cryo-electron and TIRF microscopy. Ward AE, Kiessling V, Pornillos O, White JM, Ganser-Pornillos BK, Tamm LK.J Biol Chem. 2020 Nov 6;295(45):15183-15195. doi: 10.1074/jbc.RA120.014466. Epub 2020 Aug 11.PMID: 32788212 (

Synaptotagmin-7 enhances calcium-sensing of chromaffin cell granules and slows discharge of granule cargos. Bendahmane M, Morales A, Kreutzberger AJB, Schenk NA, Mohan R, Bakshi S, Philippe JM, Zhang S, Kiessling V, Tamm LK, Giovannucci DR, Jenkins PM, Anantharam A.J Neurochem. 2020 Sep;154(6):598-617. doi: 10.1111/jnc.14986. Epub 2020 Mar 9.PMID: 32058590 (

A molecular mechanism for calcium-mediated synaptotagmin-triggered exocytosis. Kiessling V, Kreutzberger AJB, Liang B, Nyenhuis SB, Seelheim P, Castle JD, Cafiso DS, Tamm LK.Nat Struct Mol Biol. 2018 Oct;25(10):911-917. doi: 10.1038/s41594-018-0130-9. Epub 2018 Oct 5.PMID: 30291360 (

Quaternary structure of the small amino acid transporter OprG from Pseudomonas aeruginosa. Sanganna Gari RR, Seelheim P, Marsh B, Kiessling V, Creutz CE, Tamm LK.J Biol Chem. 2018 Nov 2;293(44):17267-17277. doi: 10.1074/jbc.RA118.004461. Epub 2018 Sep 20.PMID: 30237175 (

Distinct reaction mechanisms for hyaluronan biosynthesis in different kingdoms of life.  Blackburn MR, Hubbard C, Kiessling V, Bi Y, Kloss B, Tamm LK, Zimmer J.Glycobiology. 2018 Feb 1;28(2):108-121. doi: 10.1093/glycob/cwx096.PMID: 29190396  (

In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties. Kreutzberger AJB, Kiessling V, Stroupe C, Liang B, Preobraschenski J, Ganzella M, Kreutzberger MAB, Nakamoto R, Jahn R, Castle JD, Tamm LK.  Nat Commun. 2019 Aug 29;10(1):3904. doi: 10.1038/s41467-019-11873-8. (

Asymmetric Phosphatidylethanolamine Distribution Controls Fusion Pore Lifetime and Probability. Kreutzberger AJB, Kiessling V, Liang B, Yang ST, Castle JD, Tamm LK. Biophys J. 2017 Oct 13. pii: S0006-3495(17)31026-3. doi: 10.1016/j.bpj.2017.09.014. [Epub ahead of print](

Reconstitution of calcium-mediated exocytosis of dense-core vesicles. Kreutzberger AJB, Kiessling V, Liang B, Seelheim P, Jakhanwal S, Jahn R, Castle JD, Tamm LK.  Science Advances 19 Jul 2017: Vol. 3. No. 7. E1603208 DOI:10.1126/sciadv.1603208 (

HIV virions sense plasma membrane heterogeneity for cell entry. Yang ST, Kreutzberger AJB, Kiessling V, Ganser-Pornillos BK, White JM, Tamm LK. (2017)  Sci. Adv. Jun 2017: Vol. 3, no. 6, e1700338(

Complexin Binding to Membranes and Acceptor t-SNAREs Explains Its Clamping Effect on Fusion. Zdanowicz R, Kreutzberger A, Liang B, Kiessling V, Tamm LK, Cafiso DS.  Biophys J. 2017 Apr 26. pii: S0006-3495(17)30389-2. doi: 10.1016/j.bpj.2017.04.002. [Epub ahead of print]

Planar Supported Membranes with Mobile SNARE Proteins and Quantitative Fluorescence Microscopy Assays to Study Synaptic Vesicle Fusion. Kiessling V, Liang B, Kreutzberger AJ, Tamm LK.  Front Mol Neurosci. 2017 Mar 16;10:72. doi: 10.3389/fnmol.2017.00072. eCollection 2017. (

Assembly and Comparison of Plasma Membrane SNARE Acceptor Complexes. Kreutzberger AJ, Liang B, Kiessling V, Tamm LK.  Biophys J. (2016) May 24;110(10):2147-50. (

The role of cholesterol in membrane fusion. Yang ST, Kreutzberger AJ, Lee J, Kiessling V, Tamm LK.  Chem Phys Lipids. (2016) May 11. pii: S0009-3084(16)30055-X. (

Line tension at lipid phase boundaries as driving force for HIV fusion peptide-mediated fusion. Yang ST, Kiessling V, Tamm LK.   Nat Commun. (2016) Apr 26;7:11401. (

High Cholesterol Obviates a Prolonged Hemifusion Intermediate in Fast SNARE-Mediated Membrane Fusion. Kreutzberger AJ, Kiessling V, Tamm LK  Biophys J. (2015) Jul 21;109(2):319-29. (

Reconstituting SNARE-mediated membrane fusion at the single liposome level. Kiessling V, Liang B, Tamm LK.  Methods Cell Biol. (2015) 128:339-63.

Variable cooperativity in SNARE-mediated membrane fusion. Hernandez, J.M., Kreutzberger, A.J., Kiessling, V., Tamm, L.K., Jahn, R.  Proc Natl Acad Sci USA. Aug 19;111(33);12037-42 (2014). (

The Juxtamembrane Linker of Full-length Synaptotagmin 1 Controls Oligomerization and Calcium-dependent Membrane Binding. Lu, B., Kiessling, V., Tamm, L.K., Cafiso, D.S.   J Biol Chem.  Aug 8;289(32):22161-71 (2014). (

Regulation of Rac Translocation and Activation by Membrane Domains and Their Boundaries. Moissoglu, K., Kiessling, V., Wan, C., Hoffman, B.D., Norambuena, A., Tamm, L.K., Schwartz, M.A. Journal of Cell Science. (2014) (In Press). (

Prefusion structure of syntaxin-1A suggests pathway for folding into neuronal trans-SNARE complex fusion intermediate. Liang, B., Kiessling, V., Tamm, L.K.,  Proc Natl Acad Sci USA. Nov 26; 110 (48):19348-9 (2013).(

Rapid Fusion of Synaptic Vesicles with Reconstituted Target SNARE Membranes. Kiessling, V.,  Ahmed, S.,  Domanska, M.,  Holt, M.,  Jahn, R., Tamm, L.K.  Biophysical Journal 104:1950-1958 (2013).(

Transbilayer coupling of lipid dynamics. Kiessling, V.  Biophys J. Dec. 19;103(12):2409-10 (2012). (

Partitioning of Synaptotagamin 1 C2 Domains between Liquid-Ordered and Liquid-Disordered Inner Leaflet Lipid Phases. Wan, C., Kiessling, V., Cafiso, D.S., & Tamm, L.K.  Biochemistry 50: 2478-2485 (2011). (

Morphological changes induced by the action of antimicrobial peptides on supported lipid bilayers. Arouri, A., Kiessling, V.,  Tamm, L.K., Dathe, M. & Blume, A.  Journal of Physical Chemistry B 115: 158-167 (2011). (

Single SNARE-mediated vesicle fusion observed in vitro by polarized TIRFM. Kiessling, V., Domanska, M.K. & Tamm, L.K.   Biophysical Journal 99: 4047-4055 (2010). (

Docking and fast fusion of synaptobrevin vesicles depends on the lipid compositions of the vesicle and the acceptor SNARE complex-containing target membrane. Domanska, M.K., Kiessling, V. & Tamm, L.K. Biophysical Journal 99: 2936-2946 (2010). (

Single Vesicle Millisecond Fusion Kinetics Reveals Number of SNARE Complexes Optimal for Fast SNARE-mediated Membrane Fusion. Domanska, M.K., Kiessling, V., Stein, A., Fasshauer, D. & Tamm, L.T.   Journal of Biological Chemistry. 284(46): 32158-32166 (2009). (

Supported Double Membranes.  Murray, D.H., Tamm, L.K., Kiessling, V.  J. Structural Biology 168, 183-189 (2009). (

Domain coupling in asymmetric lipid bilayers. Kiessling, V., Wan, C., & Tamm, L.K.  Biochimica et Biophysica Acta, 1788: 64-71 (2009). (

Coupling of Cholesterol-Rich Lipid Phases in Assymmetric Bilayers. Wan, C., Kiessling, V., & Tamm, L.K. Biochemistry 47: 2190-2198 (2008). (

Supported Lipid Bilayers: Development and Application in Chemical Biology.  Kiessling, V., Domanska, M.K., Murray, D., Wan, C., and Tamm L.K. Wiley Encyclopedia of Chemical Biology, John Wiley & Sons, Hoboken, Volume 4, pp. 411-422 (2008). (Full text in PDF)

Transbilayer Effects of Raft-Like Lipid Domains in Asymmetric Planar Bilayer Measured by Single Molecule Tracking. Kiessling V., Crane, J.M., Tamm L.K.   Biophys J. 91: 3313-3326 (2006). Erratum in: Biophys J. Jan 15;92(2):698 (2007). (

Imaging Fast SNARE Mediated-Membrane Fusion in Planar-Supported Bilayers. Kiessling, V.  Biophysical Journal. 89: 2185-2186 (2005). (

Potassium Channel Gating in Adhesion: from an Oocyte-Silicon to a Neuron-Astrocyte Adhesion Contact. Kiessling, V., Vassanelli, S.   Eur Biophys J. 34: 113-126 (2005). (

Measuring Lipid Asymmetry In Planar Supported Bilayers by Fluorescence Interference Contrast Micorscopy. Crane, J.M., Kiessling, V. & Tamm, L.K.  Langmuir. 21: 1377-1388 (2005). (

Membrane fusion: a structural perspective on the interplay of lipids and proteins.  Tamm, L.K., Crane, J. & Kiessling, V. (Review) Curr. Op. Struct. Biol. 13: 453-466 (2003). (

Measuring distances in supported bilayers by fluorescence interference-contrast microscopy: polymer supports and SNARE proteins. Kiessling, V., Tamm, L.K.   Biophys. J. 84: 408-418 (2003). (

Membrane Dynamics.Tamm, L.K., Kiessling, V. & Wagner, M.L.  Encyclopedia of Life Sciences. Nature Publishing Group, Macmillan Publishers, Ltd. (2001). (

Extracellular Resistance in Cell Adhesion measured with a Transistor Probe Kiessling, V., Müller, B., Fromherz, P.  . Langmuir. 16(7): 3517-3521 (2000).

Membrane transistor with giant lipid vesicle touching a silicon chip Fromherz, P., Kiessling, V., Kottig, K., Zeck, G. . Applied Physics A. 69(5): 571-576 (1999).