Auble, David T.
Professor, Biochemistry and Molecular Genetics
- AB, Biochemistry, Bowdoin College
- PhD, Biochemistry, Case Western Reserve University
- Postdoc, Molecular Biology, Fred Hutchinson Cancer Research Center
Biochemistry, Bioinformatics and Genomics, Biophysics, Biophysics & Structural Biology, Epigenetics, Genetics, Molecular Biology
Molecular Mechanisms of Transcriptional Regulation
We have a longstanding interest in transcription and chromatin. The work in the Auble lab is multidisciplinary, including biochemical, biophysical, molecular biological, genetic and genomic approaches to understanding fundamental molecular mechanisms controlling transcription in eukaryotic cells, using budding yeast and human cells as model systems. A major focus is to understand how transcriptional regulatory states are established and maintained on a molecular level. To understand molecular mechanisms by which regulatory changes give rise to altered levels of RNA in real time, we are developing and applying methods for measuring chromatin-binding dynamics, which can occur on a broad time scale, from seconds to hours or more. Budding yeast offer an ideal system which allows us to employ the full armament of yeast tools and methodologies to this problem. In parallel, we also using genomic approaches to document and understand expression and epigenetic signatures in primary human cells, as well as mouse and rat cell lines and tissues. The goal is to determine how epigenetic modifications impact health and disease by reinforcing stable changes in gene expression that may persist for the life of an individual or even across generations. Thus, the overarching goal of our research program is to understand transcriptional dynamics as it occurs on all time scales, from seconds, to minutes, hours or even days and years.