David S. Wilkes

Md-Dmed School of Medicine Adm

Primary Location

PO Box 800796
Charlottesville, VA

Primary Phone Number

434.982.4050

Fax Number

434.982.0874

[email protected]

Bio & Overview

Research studies are focused on unraveling the immune mechanisms that lead to allograft destruction. Lung transplantation is a commonly utilized modality for the treatment of end stage pulmonary diseases. However, the lung is rejected more commonly than other solid organ allografts. Repeated acute rejection episodes result in chronic rejection known as bronchiolitis obliterans, the leading cause of death in lung allograft recipients. Presentation of donor major histocompatibility complex (MHC) antigens by antigen presenting cells, such as macrophages and dendritic cells, to recipient lymphocytes is the stimulus for rejection episodes. Conversely, allograft acceptance may be induced by immunizing the recipient, orally, with donor-derived MHC proteins/peptides or MHC-"like" proteins prior to transplantation. This phenomenon, known as oral tolerance, has been shown to prevent rejection in allografts other than the lung, but has not been examined in lung allograft rejection. Although donor MHC-antigens are the stimulus and target of the rejection response, we have reported recently that type V collagen [col(V)], which may be MHC-"like", is also recognized as an antigen during lung allograft rejection in humans, and the local immune response to lung alloantigens in mice. Since col(V) is highly conserved and non-polymorphic then this indicates that lung allograft rejection is a disease of allo- and autoimmunity. Regulatory T cells (Tregs) have a key role in suppressing autoimmune diseases. As such, the autoimmune response to col(V) indicates Treg dysfunction during the rejection response. Treg suppression of immunity is also in part dependent on interactions with dendritic cells (DCs) by upregulating indoleamine 2,3 dioxygenase (IDO) in DC, an enzyme that catabolizes tryptophan resulting in suppression of T cell function. The Wilkes laboratory uses a model of lung transplantation in rats, and several murine models, as well as in vitro studies to examine: Mechanisms of col(V)-induced tolerance to lung transplants, induction of regulatory T cells in this process, the signaling cascades unique to regulatory T cells, and the regulation of IDO expression in lung dendritic cells. It is the ultimate goal of these studies to understand lung allograft rejection as the molecular level such that therapeutic modalities may be developed to improve the survival of the lung transplant recipient.

Academic Information

Department: Md-Dmed School of Medicine Adm

Primary Education

BS, Biology, Villanova University, 1978
MD, Temple University, 1982
Intern, Temple University Hospital, Philadelphia, PA, 1983
Resident, Temple University Hospital, Philadelphia, PA, 1985
Fellowship, University of Texas Sourhwestern Medical Center, 1992

Other Appointments

Medicine- Pulmonary and Critical Care Medicine

Other Affiliations

BIMS

Research Interests

Immunologically mediated lung disease

Selected Publications

Weber DJ, Allette YM, Wilkes DS, White FA, The HMGB1-RAGE Inflammatory Pathway: Implications for Brain Injury-Induced Pulmonary Dysfunction., 2015; Antioxidants & redox signaling. () . PMID: 25751601

Chang TW, Gracon AS, Murphy MP, Wilkes DS, Exploring autoimmunity in the pathogenesis of abdominal aortic aneurysms., 2015; American journal of physiology. Heart and circulatory physiology. 309(5) H719-27. PMID: 26116712

Wu Q, Gupta PK, Suzuki H, Wagner SR, Zhang C, Cummings OW, Fan L, Kaplan MH, Wilkes DS, Shilling RA, CD4 T Cells but Not Th17 Cells Are Required for Mouse Lung Transplant Obliterative Bronchiolitis., 2015; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 15(7) 1793-804. PMID: 25773063

Shah RJ, Diamond JM, Cantu E, Flesch J, Lee JC, Lederer DJ, Lama VN, Orens J, Weinacker A, Wilkes DS, Roe D, Bhorade S, Wille KM, Ware LB, Palmer SM, Crespo M, Demissie E, Sonnet J, Shah A, Kawut SM, Bellamy SL, Localio AR, Christie JD, Objective Estimates Improve Risk Stratification for Primary Graft Dysfunction after Lung Transplantation., 2015; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 15(8) 2188-96. PMID: 25877792

Wilkes DS, Chew T, Flaherty KR, Frye S, Gibson KF, Kaminski N, Klemsz MJ, Lange W, Noth I, Rothhaar K, Oral immunotherapy with type V collagen in idiopathic pulmonary fibrosis., 2015; The European respiratory journal. 45(5) 1393-402. PMID: 25614165

Shah RJ, Wickersham N, Lederer DJ, Palmer SM, Cantu E, Diamond JM, Kawut SM, Lama VN, Bhorade S, Crespo M, Demissie E, Sonett J, Wille K, Orens J, Weinacker A, Shah P, Arcasoy S, Wilkes DS, Christie JD, Ware LB, Preoperative plasma club (clara) cell secretory protein levels are associated with primary graft dysfunction after lung transplantation., 2014; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 14(2) 446-52. PMID: 24400993 | PMCID: PMC3946770

Burlingham W, Wilkes DS, Sullivan JA, Why is the patient out of breath? Collagen V(α1) and K-α1-tubulin take center stage in lung transplantation., 2014; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 14(10) 2201-3. PMID: 25220400

Ardery NL, Krol DM, Wilkes DS, Leveraging diversity in American academic medicine. The Harold Amos medical faculty development program., 2014; Annals of the American Thoracic Society. 11(4) 600-2. PMID: 24708173

Pandya PH, Wilkes DS, Complement system in lung disease., 2014; American journal of respiratory cell and molecular biology. 51(4) 467-73. PMID: 24901241 | PMCID: PMC4189484

Sullivan JA, Jankowska-Gan E, Shi L, Roenneburg D, Hegde S, Greenspan DS, Wilkes DS, Denlinger LC, Burlingham WJ, Differential requirement for P2X7R function in IL-17 dependent vs. IL-17 independent cellular immune responses., 2014; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 14(7) 1512-22. PMID: 24866539 | PMCID: PMC4295495

Shah RJ, Emtiazjoo AM, Diamond JM, Smith PA, Roe DW, Wille KM, Orens JB, Ware LB, Weinacker A, Lama VN, Bhorade SM, Palmer SM, Crespo M, Lederer DJ, Cantu E, Eckert GJ, Christie JD, Wilkes DS, Plasma complement levels are associated with primary graft dysfunction and mortality after lung transplantation., 2014; American journal of respiratory and critical care medicine. 189(12) 1564-7. PMID: 24930532 | PMCID: PMC4226014

Braun NA, Celada LJ, Herazo-Maya JD, Abraham S, Shaginurova G, Sevin CM, Grutters J, Culver DA, Dworski R, Sheller J, Massion PP, Polosukhin VV, Johnson JE, Kaminski N, Wilkes DS, Oswald-Richter KA, Drake WP, Blockade of the programmed death-1 pathway restores sarcoidosis CD4(+) T-cell proliferative capacity., 2014; American journal of respiratory and critical care medicine. 190(5) 560-71. PMID: 25073001 | PMCID: PMC4214083

Weber DJ, Gracon AS, Ripsch MS, Fisher AJ, Cheon BM, Pandya PH, Vittal R, Capitano ML, Kim Y, Allette YM, Riley AA, McCarthy BP, Territo PR, Hutchins GD, Broxmeyer HE, Sandusky GE, White FA, Wilkes DS, The HMGB1-RAGE axis mediates traumatic brain injury-induced pulmonary dysfunction in lung transplantation., 2014; Science translational medicine. 6(252) 252ra124. PMID: 25186179

Gu H, Mickler EA, Cummings OW, Sandusky GE, Weber DJ, Gracon A, Woodruff T, Wilkes DS, Vittal R, Crosstalk between TGF-β1 and complement activation augments epithelial injury in pulmonary fibrosis., 2014; FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 28(10) 4223-34. PMID: 24958208 | PMCID: PMC4202097

Meyer KC, Raghu G, Verleden GM, Corris PA, Aurora P, Wilson KC, Brozek J, Glanville AR, An international ISHLT/ATS/ERS clinical practice guideline: diagnosis and management of bronchiolitis obliterans syndrome., 2014; The European respiratory journal. 44(6) 1479-503. PMID: 25359357

Gracon AS, Wilkes DS, Lung transplantation: chronic allograft dysfunction and establishing immune tolerance., 2014; Human immunology. 75(8) 887-94. PMID: 24979671 | PMCID: PMC4357397

Vittal R, Mickler EA, Fisher AJ, Zhang C, Rothhaar K, Gu H, Brown KM, Emtiazdjoo A, Lott JM, Frye SB, Smith GN, Sandusky GE, Cummings OW, Wilkes DS, Type V collagen induced tolerance suppresses collagen deposition, TGF-β and associated transcripts in pulmonary fibrosis., 2013; PloS one. 8(10) e76451. PMID: 24204629 | PMCID: PMC3804565

Lott JM, Sehra S, Mehrotra P, Mickler EA, Fisher AJ, Zhang W, Presson RG, Busk MF, Goenka S, Gunst SJ, Kaplan MH, Wilkes DS, Wenzel SE, Type V collagen-induced tolerance prevents airway hyperresponsiveness., 2013; American journal of respiratory and critical care medicine. 187(4) 454-7. PMID: 23418334 | PMCID: PMC3603593

Diamond JM, Lee JC, Kawut SM, Shah RJ, Localio AR, Bellamy SL, Lederer DJ, Cantu E, Kohl BA, Lama VN, Bhorade SM, Crespo M, Demissie E, Sonett J, Wille K, Orens J, Shah AS, Weinacker A, Arcasoy S, Shah PD, Wilkes DS, Ware LB, Palmer SM, Christie JD, Clinical risk factors for primary graft dysfunction after lung transplantation., 2013; American journal of respiratory and critical care medicine. 187(5) 527-34. PMID: 23306540 | PMCID: PMC3733407

Vittal R, Fisher A, Gu H, Mickler EA, Panitch A, Lander C, Cummings OW, Sandusky GE, Wilkes DS, Peptide-mediated inhibition of mitogen-activated protein kinase-activated protein kinase-2 ameliorates bleomycin-induced pulmonary fibrosis., 2013; American journal of respiratory cell and molecular biology. 49(1) 47-57. PMID: 23470623 | PMCID: PMC3727887

Yao W, Zhang Y, Jabeen R, Nguyen ET, Wilkes DS, Tepper RS, Kaplan MH, Zhou B, Interleukin-9 is required for allergic airway inflammation mediated by the cytokine TSLP., 2013; Immunity. 38(2) 360-72. PMID: 23376058 | PMCID: PMC3582776

Diamond JM, Porteous MK, Cantu E, Meyer NJ, Shah RJ, Lederer DJ, Kawut SM, Lee J, Bellamy SL, Palmer SM, Lama VN, Bhorade SM, Crespo M, Demissie E, Wille K, Orens J, Shah PD, Weinacker A, Weill D, Arcasoy S, Wilkes DS, Ware LB, Christie JD, Elevated plasma angiopoietin-2 levels and primary graft dysfunction after lung transplantation., 2013; PloS one. 7(12) e51932. PMID: 23284823 | PMCID: PMC3526525

Walline CC, Sehra S, Fisher AJ, Guindon LM, Kratzke IM, Montgomery JB, Lipking KP, Glosson NL, Benson HL, Sandusky GE, Wilkes DS, Brutkiewicz RR, Kaplan MH, Blum JS, Allergic airway disease in mice alters T and B cell responses during an acute respiratory poxvirus infection., 2013; PloS one. 8(4) e62222. PMID: 23620814 | PMCID: PMC3631162

Shah RJ, Diamond JM, Cantu E, Lee JC, Lederer DJ, Lama VN, Orens J, Weinacker A, Wilkes DS, Bhorade S, Wille KM, Ware LB, Palmer SM, Crespo M, Localio AR, Demissie E, Kawut SM, Bellamy SL, Christie JD, Latent class analysis identifies distinct phenotypes of primary graft dysfunction after lung transplantation., 2013; Chest. 144(2) 616-22. PMID: 23429890 | PMCID: PMC3734891

Suzuki H, Lasbury ME, Fan L, Vittal R, Mickler EA, Benson HL, Shilling R, Wu Q, Weber DJ, Wagner SR, Lasaro M, Devore D, Wang Y, Sandusky GE, Lipking K, Pandya P, Reynolds J, Love R, Wozniak T, Gu H, Brown KM, Wilkes DS, Role of complement activation in obliterative bronchiolitis post-lung transplantation., 2013; Journal of immunology (Baltimore, Md. : 1950). 191(8) 4431-9. PMID: 24043901 | PMCID: PMC3873138

Oswald-Richter KA, Richmond BW, Braun NA, Isom J, Abraham S, Taylor TR, Drake JM, Culver DA, Wilkes DS, Drake WP, Reversal of global CD4+ subset dysfunction is associated with spontaneous clinical resolution of pulmonary sarcoidosis., 2013; Journal of immunology (Baltimore, Md. : 1950). 190(11) 5446-53. PMID: 23630356 | PMCID: PMC3660530

Raghu G, Anstrom KJ, King TE, Lasky JA, Martinez FJ, Prednisone, azathioprine, and N-acetylcysteine for pulmonary fibrosis., 2012; The New England journal of medicine. 366(21) 1968-77. PMID: 22607134 | PMCID: PMC3422642

Emtiazjoo AM, Wilkes DS, Humoral immunity and the development of obliterative bronchiolitis after lung transplantation: is there a link?, 2012; American journal of respiratory cell and molecular biology. 48(2) 145-9. PMID: 23087052 | PMCID: PMC3604061

Weber DJ, Wilkes DS, The role of autoimmunity in obliterative bronchiolitis after lung transplantation., 2012; American journal of physiology. Lung cellular and molecular physiology. 304(5) L307-11. PMID: 23262227 | PMCID: PMC3602736

Vittal R, Fan L, Greenspan DS, Mickler EA, Gopalakrishnan B, Gu H, Benson HL, Zhang C, Burlingham W, Cummings OW, Wilkes DS, IL-17 induces type V collagen overexpression and EMT via TGF-β-dependent pathways in obliterative bronchiolitis., 2012; American journal of physiology. Lung cellular and molecular physiology. 304(6) L401-14. PMID: 23262228 | PMCID: PMC3602743

Benson HL, Suzuki H, Lott J, Fisher AJ, Walline C, Heidler KM, Brutkiewicz R, Blum JS, Wilkes DS, Donor lung derived myeloid and plasmacytoid dendritic cells differentially regulate T cell proliferation and cytokine production., 2012; Respiratory research. 13() 25. PMID: 22433165 | PMCID: PMC3352265

Diab KJ, Yu Z, Wood KL, Shmalo JA, Sheski FD, Farber MO, Wilkes DS, Nelson RP, Comparison of pulmonary complications after nonmyeloablative and conventional allogeneic hematopoietic cell transplant., 2012; Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 18(12) 1827-34. PMID: 22766224

Shah RJ, Bellamy SL, Localio AR, Wickersham N, Diamond JM, Weinacker A, Lama VN, Bhorade S, Belperio JA, Crespo M, Demissie E, Kawut SM, Wille KM, Lederer DJ, Lee JC, Palmer SM, Orens J, Reynolds J, Shah A, Wilkes DS, Ware LB, Christie JD, A panel of lung injury biomarkers enhances the definition of primary graft dysfunction (PGD) after lung transplantation., 2012; The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation. 31(9) 942-9. PMID: 22694851 | PMCID: PMC3418416

Suzuki H, Fan L, Wilkes DS, Development of obliterative bronchiolitis in a murine model of orthotopic lung transplantation., 2012; Journal of visualized experiments : JoVE. (65) . PMID: 22805894 | PMCID: PMC3476401

Diamond JM, Meyer NJ, Feng R, Rushefski M, Lederer DJ, Kawut SM, Lee JC, Cantu E, Shah RJ, Lama VN, Bhorade S, Crespo M, Demissie E, Sonett J, Wille K, Orens J, Weinacker A, Weill D, Arcasoy S, Shah PD, Belperio JA, Wilkes D, Ware LB, Palmer SM, Christie JD, Variation in PTX3 is associated with primary graft dysfunction after lung transplantation., 2012; American journal of respiratory and critical care medicine. 186(6) 546-52. PMID: 22822025 | PMCID: PMC3480532

Benson HL, Wilkes DS, Matrix metalloproteinases in T cell mediated pulmonary diseases., 2011; Frontiers in bioscience (Elite edition). 4() 2162-9. PMID: 22202028

Feghali-Bostwick CA, Wilkes DS, Autoimmunity in idiopathic pulmonary fibrosis: are circulating autoantibodies pathogenic or epiphenomena?, 2011; American journal of respiratory and critical care medicine. 183(6) 692-3. PMID: 21471064

Shekhar A, Denne S, Tierney W, Wilkes D, Brater DC, A model for engaging public-private partnerships., 2011; Clinical and translational science. 4(2) 80-3. PMID: 21463491

Wilkes DS, Autoantibody formation in human and rat studies of chronic rejection and primary graft dysfunction., 2011; Seminars in immunology. 24(2) 131-5. PMID: 21925897 | PMCID: PMC3873136

Fan L, Benson HL, Vittal R, Mickler EA, Presson R, Fisher AJ, Cummings OW, Heidler KM, Keller MR, Burlingham WJ, Wilkes DS, Neutralizing IL-17 prevents obliterative bronchiolitis in murine orthotopic lung transplantation., 2011; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 11(5) 911-22. PMID: 21521466 | PMCID: PMC3083638

Diamond JM, Lederer DJ, Kawut SM, Lee J, Ahya VN, Bellamy S, Palmer SM, Lama VN, Bhorade S, Crespo M, Demissie E, Sonett J, Wille K, Orens J, Shah PD, Weinacker A, Weill D, Kohl BA, Deutschman CC, Arcasoy S, Shah AS, Belperio JA, Wilkes D, Reynolds JM, Ware LB, Christie JD, Elevated plasma long pentraxin-3 levels and primary graft dysfunction after lung transplantation for idiopathic pulmonary fibrosis., 2011; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 11(11) 2517-22. PMID: 21883907 | PMCID: PMC3206646

Wilkes DS, Chronic lung allograft rejection and airway microvasculature: is HIF-1 the missing link?, 2011; The Journal of clinical investigation. 121(6) 2155-7. PMID: 21606603 | PMCID: PMC3104786

Shilling RA, Wilkes DS, Role of Th17 cells and IL-17 in lung transplant rejection., 2011; Seminars in immunopathology. 33(2) 129-34. PMID: 21279808 | PMCID: PMC3945469

Gibau GS, Foertsch J, Blum J, Brutkiewicz R, Queener S, Roman A, Rhodes S, Sturek M, Wilkes D, Broxmeyer H, Diversifying Biomedical Training: A Synergistic Intervention., 2011; Journal of women and minorities in science and engineering. 16(3) 215-235. PMID: 21796238 | PMCID: PMC3143029

Dart ML, Jankowska-Gan E, Huang G, Roenneburg DA, Keller MR, Torrealba JR, Rhoads A, Kim B, Bobadilla JL, Haynes LD, Wilkes DS, Burlingham WJ, Greenspan DS, Interleukin-17-dependent autoimmunity to collagen type V in atherosclerosis., 2010; Circulation research. 107(9) 1106-16. PMID: 20814021 | PMCID: PMC3010213

Benson HL, Mobashery S, Chang M, Kheradmand F, Hong JS, Smith GN, Shilling RA, Wilkes DS, Endogenous matrix metalloproteinases 2 and 9 regulate activation of CD4+ and CD8+ T cells., 2010; American journal of respiratory cell and molecular biology. 44(5) 700-8. PMID: 20639459 | PMCID: PMC3095987

Wilkes DS, A breath of fresh air for lung transplant recipients., 2010; Science translational medicine. 1(4) 4ps5. PMID: 20368169

Wilkes DS, Airway hypoxia, bronchiolar artery revascularization, and obliterative bronchiolitis/bronchiolitis obliterans syndrome: are we there yet?, 2010; American journal of respiratory and critical care medicine. 182(2) 136-7. PMID: 20634500

Qu P, Du H, Wilkes DS, Yan C, Critical roles of lysosomal acid lipase in T cell development and function., 2009; The American journal of pathology. 174(3) 944-56. PMID: 19179613 | PMCID: PMC2665754

Braun RK, Molitor-Dart M, Wigfield C, Xiang Z, Fain SB, Jankowska-Gan E, Seroogy CM, Burlingham WJ, Wilkes DS, Brand DD, Torrealba J, Love RB, Transfer of tolerance to collagen type V suppresses T-helper-cell-17 lymphocyte-mediated acute lung transplant rejection., 2009; Transplantation. 88(12) 1341-8. PMID: 20029330 | PMCID: PMC2804859

Arefayene M, Philips S, Cao D, Mamidipalli S, Desta Z, Flockhart DA, Wilkes DS, Skaar TC, Identification of genetic variants in the human indoleamine 2,3-dioxygenase (IDO1) gene, which have altered enzyme activity., 2009; Pharmacogenetics and genomics. 19(6) 464-76. PMID: 19514129

Yamada Y, Sekine Y, Yoshida S, Yasufuku K, Petrache I, Benson HL, Brand DD, Yoshino I, Wilkes DS, Type V collagen-induced oral tolerance plus low-dose cyclosporine prevents rejection of MHC class I and II incompatible lung allografts., 2009; Journal of immunology (Baltimore, Md. : 1950). 183(1) 237-45. PMID: 19542435 | PMCID: PMC2997997

Shilling RA, Wilkes DS, Immunobiology of chronic lung allograft dysfunction: new insights from the bench and beyond., 2009; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 9(8) 1714-8. PMID: 19519813 | PMCID: PMC2734467

Wilkes DS, Primary graft dysfunction: it's all the RAGE., 2009; American journal of respiratory and critical care medicine. 180(10) 915-6. PMID: 19897768

Khan BA, Duncan M, Reynolds J, Wilkes DS, Nocardia infection in lung transplant recipients., 2008; Clinical transplantation. 22(5) 562-6. PMID: 18435787 | PMCID: PMC3755737

Bobadilla JL, Love RB, Jankowska-Gan E, Xu Q, Haynes LD, Braun RK, Hayney MS, Munoz del Rio A, Meyer K, Greenspan DS, Torrealba J, Heidler KM, Cummings OW, Iwata T, Brand D, Presson R, Burlingham WJ, Wilkes DS, Th-17, monokines, collagen type V, and primary graft dysfunction in lung transplantation., 2008; American journal of respiratory and critical care medicine. 177(6) 660-8. PMID: 18174545 | PMCID: PMC2267340

Iwata T, Chiyo M, Yoshida S, Smith GN, Mickler EA, Presson R, Fisher AJ, Brand DD, Cummings OW, Wilkes DS, Lung transplant ischemia reperfusion injury: metalloprotease inhibition down-regulates exposure of type V collagen, growth-related oncogene-induced neutrophil chemotaxis, and tumor necrosis factor-alpha expression., 2008; Transplantation. 85(3) 417-26. PMID: 18322435

Murata K, Iwata T, Nakashima S, Fox-Talbot K, Qian Z, Wilkes DS, Baldwin WM, C4d deposition and cellular infiltrates as markers of acute rejection in rat models of orthotopic lung transplantation., 2008; Transplantation. 86(1) 123-9. PMID: 18622289 | PMCID: PMC2659637

Judson MA, Baughman RP, Costabel U, Flavin S, Lo KH, Kavuru MS, Drent M, Efficacy of infliximab in extrapulmonary sarcoidosis: results from a randomised trial., 2008; The European respiratory journal. 31(6) 1189-96. PMID: 18256069

Iwata T, Philipovskiy A, Fisher AJ, Presson RG, Chiyo M, Lee J, Mickler E, Smith GN, Petrache I, Brand DB, Burlingham WJ, Gopalakrishnan B, Greenspan DS, Christie JD, Wilkes DS, Anti-type V collagen humoral immunity in lung transplant primary graft dysfunction., 2008; Journal of immunology (Baltimore, Md. : 1950). 181(8) 5738-47. PMID: 18832733 | PMCID: PMC2997998

Chiyo M, Iwata T, Webb TJ, Vasko MR, Thompson EL, Heidler KM, Cummings OW, Yoshida S, Fujisawa T, Brand DD, Wilkes DS, Silencing S1P1 receptors regulates collagen-V reactive lymphocyte-mediated immunobiology in the transplanted lung., 2008; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 8(3) 537-46. PMID: 18294150

Moss M, Wilkes D, Ware LB, Clinical year in review II: Pulmonary infections in the immunocompetent host, issues in the training of fellows and residents, asthma, and chronic obstructive pulmonary disease., 2008; Proceedings of the American Thoracic Society. 5(7) 745-50. PMID: 18757311

Wilkes DS, Clinical year in review III: Idiopathic pulmonary fibrosis, occupational medicine, and lung transplantation., 2008; Proceedings of the American Thoracic Society. 5(7) 751-4. PMID: 18757312

Kahi CJ, Saxena R, Temkit M, Canlas K, Roberts S, Knox K, Wilkes D, Kwo PY, Hepatobiliary disease in sarcoidosis., 2007; Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG / World Association of Sarcoidosis and Other Granulomatous Disorders. 23(2) 117-23. PMID: 17937107

Smith GN, Mickler EA, Payne KK, Lee J, Duncan M, Reynolds J, Foresman B, Wilkes DS, Lung transplant metalloproteinase levels are elevated prior to bronchiolitis obliterans syndrome., 2007; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 7(7) 1856-61. PMID: 17524078

Yoshida S, Iwata T, Chiyo M, Smith GN, Foresman BH, Mickler EA, Heidler KM, Cummings OW, Fujisawa T, Brand DD, Baker A, Wilkes DS, Metalloproteinase inhibition has differential effects on alloimmunity, autoimmunity, and histopathology in the transplanted lung., 2007; Transplantation. 83(6) 799-808. PMID: 17414715

Hong L, Webb TJ, Wilkes DS, Dendritic cell-T cell interactions: CD8 alpha alpha expressed on dendritic cells regulates T cell proliferation., 2007; Immunology letters. 108(2) 174-8. PMID: 17224189 | PMCID: PMC1910694

Benichou G, Alessandrini A, Charrad RS, Wilkes DS, Induction of autoimmunity after allotransplantation., 2007; Frontiers in bioscience : a journal and virtual library. 12() 4362-9. PMID: 17485380

Yao Y, Li P, Singh P, Thiele AT, Wilkes DS, Renukaradhya GJ, Brutkiewicz RR, Travers JB, Luker GD, Hong SC, Blum JS, Chang CH, Vaccinia virus infection induces dendritic cell maturation but inhibits antigen presentation by MHC class II., 2007; Cellular immunology. 246(2) 92-102. PMID: 17678637 | PMCID: PMC2100387

Banks KE, Humphreys TL, Li W, Katz BP, Wilkes DS, Spinola SM, Haemophilus ducreyi partially activates human myeloid dendritic cells., 2007; Infection and immunity. 75(12) 5678-85. PMID: 17923525 | PMCID: PMC2168323

Burlingham WJ, Love RB, Jankowska-Gan E, Haynes LD, Xu Q, Bobadilla JL, Meyer KC, Hayney MS, Braun RK, Greenspan DS, Gopalakrishnan B, Cai J, Brand DD, Yoshida S, Cummings OW, Wilkes DS, IL-17-dependent cellular immunity to collagen type V predisposes to obliterative bronchiolitis in human lung transplants., 2007; The Journal of clinical investigation. 117(11) 3498-506. PMID: 17965778 | PMCID: PMC2040314

Sumpter TL, Payne KK, Wilkes DS, Regulation of the NFAT pathway discriminates CD4+CD25+ regulatory T cells from CD4+CD25- helper T cells., 2007; Journal of leukocyte biology. 83(3) 708-17. PMID: 18032693

Humphreys TL, Li L, Li X, Janowicz DM, Fortney KR, Zhao Q, Li W, McClintick J, Katz BP, Wilkes DS, Edenberg HJ, Spinola SM, Dysregulated immune profiles for skin and dendritic cells are associated with increased host susceptibility to Haemophilus ducreyi infection in human volunteers., 2007; Infection and immunity. 75(12) 5686-97. PMID: 17893130 | PMCID: PMC2168359

Thiele AT, Sumpter TL, Walker JA, Xu Q, Chang CH, Bacallao RL, Kher R, Wilkes DS, Pulmonary immunity to viral infection: adenovirus infection of lung dendritic cells renders T cells nonresponsive to interleukin-2., 2006; Journal of virology. 80(4) 1826-36. PMID: 16439539 | PMCID: PMC1367134

Yoshida S, Haque A, Mizobuchi T, Iwata T, Chiyo M, Webb TJ, Baldridge LA, Heidler KM, Cummings OW, Fujisawa T, Blum JS, Brand DD, Wilkes DS, Anti-type V collagen lymphocytes that express IL-17 and IL-23 induce rejection pathology in fresh and well-healed lung transplants., 2006; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 6(4) 724-35. PMID: 16539629

Roberts SD, Kohli LL, Wood KL, Wilkes DS, Knox KS, CD4+CD28-T cells are expanded in sarcoidosis., 2005; Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG / World Association of Sarcoidosis and Other Granulomatous Disorders. 22(1) 13-9. PMID: 15881275

Duncan MD, Wilkes DS, Transplant-related immunosuppression: a review of immunosuppression and pulmonary infections., 2005; Proceedings of the American Thoracic Society. 2(5) 449-55. PMID: 16322599 | PMCID: PMC2713333

Wilkes DS, Egan TM, Reynolds HY, Lung transplantation: opportunities for research and clinical advancement., 2005; American journal of respiratory and critical care medicine. 172(8) 944-55. PMID: 16020804 | PMCID: PMC2718411

Roe DW, Fehrenbacher JW, Niemeier MR, Zieger M, Labarrere C, Wilkes DS, Lung preservation: pulmonary flush route affects bronchial mucosal temperature and expression of IFN-gamma and Gro in regional lymph nodes., 2005; American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 5(5) 995-1001. PMID: 15816879

Sumpter TL, Wilkes DS, Role of autoimmunity in organ allograft rejection: a focus on immunity to type V collagen in the pathogenesis of lung transplant rejection., 2004; American journal of physiology. Lung cellular and molecular physiology. 286(6) L1129-39. PMID: 15136293

Mizobuchi T, Sekine Y, Yasufuku K, Fujisawa T, Wilkes DS, Comparison of surgical procedures for vascular and airway anastomoses that utilize a modified non-suture external cuff technique for experimental lung transplantation in rats., 2004; The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation. 23(7) 889-93. PMID: 15261185

Swanson KA, Zheng Y, Heidler KM, Zhang ZD, Webb TJ, Wilkes DS, Flt3-ligand, IL-4, GM-CSF, and adherence-mediated isolation of murine lung dendritic cells: assessment of isolation technique on phenotype and function., 2004; Journal of immunology (Baltimore, Md. : 1950). 173(8) 4875-81. PMID: 15470028

Mizobuchi T, Yasufuku K, Zheng Y, Haque MA, Heidler KM, Woods K, Smith GN, Cummings OW, Fujisawa T, Blum JS, Wilkes DS, Differential expression of Smad7 transcripts identifies the CD4+CD45RChigh regulatory T cells that mediate type V collagen-induced tolerance to lung allografts., 2003; Journal of immunology (Baltimore, Md. : 1950). 171(3) 1140-7. PMID: 12874199

Gordon SB, Miller DE, Day RB, Ferry T, Wilkes DS, Schnizlein-Bick CT, Zijlstra EE, Read RC, Molyneux ME, Twigg HL, Pulmonary immunoglobulin responses to Streptococcus pneumoniae are altered but not reduced in human immunodeficiency virus-infected Malawian adults., 2003; The Journal of infectious diseases. 188(5) 666-70. PMID: 12934182

Swanson KA, Zheng Y, Heidler KM, Mizobuchi T, Wilkes DS, CDllc+ cells modulate pulmonary immune responses by production of indoleamine 2,3-dioxygenase., 2003; American journal of respiratory cell and molecular biology. 30(3) 311-8. PMID: 12959949

Wilkes DS, The role of autoimmunity in the pathogenesis of lung allograft rejection., 2003; Archivum immunologiae et therapiae experimentalis. 51(4) 227-30. PMID: 12956431

Zhang S, Zeng G, Wilkes DS, Reed GE, McGarry RC, Eble JN, Cheng L, Dendritic cells transfected with interleukin-12 and pulsed with tumor extract inhibit growth of murine prostatic carcinoma in vivo., 2003; The Prostate. 55(4) 292-8. PMID: 12712408

Haque MA, Mizobuchi T, Yasufuku K, Fujisawa T, Brutkiewicz RR, Zheng Y, Woods K, Smith GN, Cummings OW, Heidler KM, Blum JS, Wilkes DS, Evidence for immune responses to a self-antigen in lung transplantation: role of type V collagen-specific T cells in the pathogenesis of lung allograft rejection., 2002; Journal of immunology (Baltimore, Md. : 1950). 169(3) 1542-9. PMID: 12133982

Knox KS, Behnia M, Smith LR, Vance GH, Busk M, Cummings OW, Kwo PY, Wilkes DS, Acute graft-versus-host disease of the lung after liver transplantation., 2002; Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society. 8(10) 968-71. PMID: 12360443

Yasufuku K, Heidler KM, Woods KA, Smith GN, Cummings OW, Fujisawa T, Wilkes DS, Prevention of bronchiolitis obliterans in rat lung allografts by type V collagen-induced oral tolerance., 2002; Transplantation. 73(4) 500-5. PMID: 11889419

Yasufuku K, Heidler KM, O'Donnell PW, Smith GN, Cummings OW, Foresman BH, Fujisawa T, Wilkes DS, Oral tolerance induction by type V collagen downregulates lung allograft rejection., 2001; American journal of respiratory cell and molecular biology. 25(1) 26-34. PMID: 11472972

Wilkes DS, Twigg HL, B-lymphocytes in the lung: a topic to be revisited., 2001; Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG / World Association of Sarcoidosis and Other Granulomatous Disorders. 18(1) 34-49. PMID: 11354546

Mares DC, Heidler KM, Smith GN, Cummings OW, Harris ER, Foresman B, Wilkes DS, Type V collagen modulates alloantigen-induced pathology and immunology in the lung., 2000; American journal of respiratory cell and molecular biology. 23(1) 62-70. PMID: 10873154

Heidler KM, Baker K, Woods K, Schnizlein-Bick C, Cummings OW, Sidner R, Foresman B, Wilkes DS, Instillation of allogeneic lung antigen-presenting cells deficient in expression of major histocompatibility complex class I or II antigens have differential effects on local cellular and humoral immunity and on pathology in recipient murine lungs., 2000; American journal of respiratory cell and molecular biology. 23(4) 499-505. PMID: 11017915

Sekine Y, Yasufuku K, Heidler KM, Cummings OW, Van Rooijen N, Fujisawa T, Brown J, Wilkes DS, Monocyte chemoattractant protein-1 and RANTES are chemotactic for graft infiltrating lymphocytes during acute lung allograft rejection., 2000; American journal of respiratory cell and molecular biology. 23(6) 719-26. PMID: 11104723

Twigg HL, Soliman DM, Day RB, Knox KS, Anderson RJ, Wilkes DS, Schnizlein-Bick CT, Lymphocytic alveolitis, bronchoalveolar lavage viral load, and outcome in human immunodeficiency virus infection., 1999; American journal of respiratory and critical care medicine. 159(5) 1439-44. PMID: 10228108

Wilkes DS, Bowman D, Cummings OW, Heidler KM, Allogeneic bronchoalveolar lavage cells induce the histology and immunology of lung allograft rejection in recipient murine lungs: role of intercellular adhesion molecule-1 on donor cells., 1999; Transplantation. 67(6) 890-6. PMID: 10199739

Twigg HL, Spain BA, Soliman DM, Knox K, Sidner RA, Schnizlein-Bick C, Wilkes DS, Iwamoto GK, Production of interferon-gamma by lung lymphocytes in HIV-infected individuals., 1999; The American journal of physiology. 276(2) L256-62. PMID: 9950887

Mares DC, Wilkes DS, Bronchoscopy in the diagnosis of respiratory infections., 1998; Current opinion in pulmonary medicine. 4(3) 123-9. PMID: 9675513

Wilkes DS, Thompson LK, Cummings OW, Bragg S, Heidler KM, Instillation of allogeneic lung macrophages and dendritic cells cause differential effects on local IFN-gamma production, lymphocytic bronchitis, and vasculitis in recipient murine lungs., 1998; Journal of leukocyte biology. 64(5) 578-86. PMID: 9823761

Sekine Y, Bowen LK, Heidler KM, Van Rooijen N, Brown JW, Cummings OW, Wilkes DS, Role of passenger leukocytes in allograft rejection: effect of depletion of donor alveolar macrophages on the local production of TNF-alpha, T helper 1/T helper 2 cytokines, IgG subclasses, and pathology in a rat model of lung transplantation., 1997; Journal of immunology (Baltimore, Md. : 1950). 159(8) 4084-93. PMID: 9378999

Wilkes DS, Sidner RA, Mathur PN, Niemeier M, Schwenk R, Heidler KM, Bowen LK, Preferential production of IgG2 antibodies by parenchymal lung B-lymphocytes during lung allograft rejection., 1997; Transplantation proceedings. 29(3) 1891-5. PMID: 9142313

Twigg HL, Spain BA, Soliman DM, Bowen LK, Heidler KM, Wilkes DS, Impaired IgG production in the lungs of HIV-infected individuals., 1996; Cellular immunology. 170(1) 127-33. PMID: 8660808

Pascuzzi RM, Shapiro SA, Rau AN, Schultz CE, Bowman RM, Caldemeyer KS, Wilkes DS, Sarcoid myelopathy., 1996; Journal of neuroimaging : official journal of the American Society of Neuroimaging. 6(1) 61-2. PMID: 8555667

Wilkes DS, Neimeier M, Mathur PN, Soliman DM, Twigg HL, Bowen LK, Heidler KM, Effect of human lung allograft alveolar macrophages on IgG production: immunoregulatory role of interleukin-10, transforming growth factor-beta, and interleukin-6., 1995; American journal of respiratory cell and molecular biology. 13(5) 621-8. PMID: 7576699

Wilkes DS, Heidler KM, Bowen LK, Quinlan WM, Doyle NA, Cummings OW, Doerschuk CM, Allogeneic bronchoalveolar lavage cells induce the histology of acute lung allograft rejection, and deposition of IgG2a in recipient murine lungs., 1995; Journal of immunology (Baltimore, Md. : 1950). 155(5) 2775-83. PMID: 7650403

Wilkes DS, Heidler KM, Niemeier M, Schwenk GR, Mathur PN, Breite WM, Cummings OW, Weissler JC, Increased bronchoalveolar IgG2/IgG1 ratio is a marker for human lung allograft rejection., 1994; Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 42(4) 652-9. PMID: 8521028

Wilkes DS, Weissler JC, Alloantigen-induced immunoglobulin production in human lung: differential effects of accessory cell populations on IgG synthesis., 1994; American journal of respiratory cell and molecular biology. 10(3) 339-46. PMID: 8117452

Yarbrough WC, Wilkes DS, Weissler JC, Inhibition of lymphokine-activated killer cells by human pulmonary macrophages: discordance between up-regulation of the beta chain (p75) of the interleukin-2 receptor on CD56+ cells and limited response to interleukin-2., 1994; American journal of respiratory cell and molecular biology. 10(2) 184-91. PMID: 7509162

Twigg HL, Wilkes DS, Soliman DM, Measurement of IL-6 inhibitory activity in cultured cell supernatants and lysates., 1994; The Journal of laboratory and clinical medicine. 124(2) 283-92. PMID: 8051493

Wilkes DS, Yarbrough WC, Weissler JC, Human alveolar macrophages inhibit immunoglobulin production in response to direct B cell mitogen., 1993; American journal of respiratory cell and molecular biology. 9(2) 141-7. PMID: 8338683

Yarbrough WC, Wilkes DS, Weissler JC, Human alveolar macrophages inhibit receptor-mediated increases in intracellular calcium concentration in lymphocytes., 1991; American journal of respiratory cell and molecular biology. 5(5) 411-5. PMID: 1657060