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John Nelson Campbell

John Nelson Campbell

Assistant Professor, Biology

Primary Location
Gilmer Hall, Room 483
485 McCormick Road
Charlottesville, Virginia 22904
Primary Phone Number

Bio & Overview

The Campbell lab uses single-cell transcriptomics and genetic technology to identify the cell types and signaling pathways that control appetite, digestion, and glucose metabolism. Specifically, we apply high-throughput single-cell RNA-Seq to characterize cell types and their marker genes in specific brain regions and to generate hypotheses about the functions of those cell types. These marker genes enable genetic access to each cell type of interest, through the combined use of marker-driven recombinase-expressing mouse strains and targeted injections of recombinase-dependent AAV vectors. Using these AAVs and the transgenic tools they deliver, we map, monitor, and manipulate the activity of specific neuron populations, revealing their synaptic circuitry and physiological function. The ultimate goal of this approach is to match molecularly-defined cell types and circuits to their specific roles in appetite, digestion, and glucose metabolism.

Academic Information

Department
Biology

Primary Education

  • BS, Biological Psychology, College of William and Mary, 2002
  • PhD, Neuroscience, Virginia Commonwealth University School of Medicine, 2012

Other Affiliations

  • MSTP
  • BIMS

Research Interests

The Neurobiology of Energy Balance

Research Disciplines

  • Computational Biology
  • Neuroscience

Research Opportunities

  • Bioinformatics & Genomics
  • Cellular and Molecular Metabolism
  • Computational Biology
  • Molecular and Systems Neuroscience
  • Neuroscience

PhD Programs

  • Neuroscience

Selected Publications

2026

2025

2024

Dorsal motor vagal neurons can elicit bradycardia and reduce anxiety-like behavior
iScience. 2024; 27(3): 109137. DOI:10.1016/J.ISCI.2024.109137.

Ghrelin deletion and conditional ghrelin cell ablation increase pancreatic islet size in mice
Journal of Clinical Investigation. 2024; 134(10):. DOI:10.1172/JCI182168.

Investigating the Role of Arcuate Pirt Neurons in Energy Homeostasis
Physiology. 2024; 39(S1):. DOI:10.1152/PHYSIOL.2024.39.S1.624.

Molecular cell types as functional units of the efferent vagus nerve
Seminars in Cell and Developmental Biology. 2024; 156: 210 - 218. DOI:10.1016/J.SEMCDB.2023.07.007.

Molecular connectomics reveals a glucagon-like peptide 1-sensitive neural circuit for satiety
Nature Metabolism. 2024; 6(12): 2354 - 2373. DOI:10.1038/S42255-024-01168-8.

Molecular organization of adrenergic and noradrenergic neurons in the mouse ventrolateral medulla
Physiology. 2024; 39(S1):. DOI:10.1152/PHYSIOL.2024.39.S1.1195.

Molecular Organization of Autonomic, Respiratory, and Spinally-Projecting Neurons in the Mouse Ventrolateral Medulla
Journal of Neuroscience. 2024; 44(31): e2211232024. DOI:10.1523/JNEUROSCI.2211-23.2024.

2023

Disambiguating control of the diving reflex by the nucleus ambiguus
Physiology. 2023; 38(S1):. DOI:10.1152/PHYSIOL.2023.38.S1.5729637.

Dorsal motor vagal neurons can suppress heart rate in mice
Physiology. 2023; 38(S1):. DOI:10.1152/PHYSIOL.2023.38.S1.5793744.

Genetic and Functional Identification of Cardiovagal Nucleus Ambiguus Neurons
Physiology. 2023; 38(S1):. DOI:10.1152/PHYSIOL.2023.38.S1.5732321.

Ghrelin deletion and conditional ghrelin cell ablation increase pancreatic islet size in mice
Journal of Clinical Investigation. 2023; 133(24):. DOI:10.1172/JCI169349.

Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network
Science Advances. 2023; 9(34):. DOI:10.1126/SCIADV.ADH9570.

Mapping visual functions onto molecular cell types in the mouse superior colliculus
Neuron. 2023; 111(12): 1876 - 1886.e5. DOI:10.1016/J.NEURON.2023.03.036.

2022

1315-P: Optogenetic Activation of Vagal Motor Neurons in Mice Modulates Heart Rate but Not Blood Glucose Levels
Diabetes. 2022; 71(Supplement_1):. DOI:10.2337/DB22-1315-P.

Chemogenetic activation of noradrenergic A5 neurons increases blood pressure and visceral sympathetic activity in adult rats
American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2022; 323(4): R512 - R531. DOI:10.1152/AJPREGU.00119.2022.

Food-induced dopamine signaling in AgRP neurons promotes feeding
Cell Reports. 2022; 41(9): 111718. DOI:10.1016/J.CELREP.2022.111718.

Genetic encoding of an esophageal motor circuit
Cell Reports. 2022; 39(11): 110962. DOI:10.1016/J.CELREP.2022.110962.

Sonic hedgehog-dependent recruitment of GABAergic interneurons into the developing visual thalamus
eLife. 2022; 11:. DOI:10.7554/ELIFE.79833.

2021

2020

2019

2017

2016