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Matthew A. Crawford

Matthew A. Crawford

Assistant Research Professor, Medicine- Infectious Diseases and International Health

Primary Location
PO Box 801340
345 Crispell Drive, MR-6, Rm 1704
Charlotesville, Virginia 22908
Primary Phone Number
Fax Number
434.924.0075

Bio & Overview

My current research includes investigations into the emergence and dissemination of antibiotic resistance among bacterial pathogens. Especially interesting are novel examinations regarding how modifications of lipid A that confer colistin resistance impact the function and spread of other clinically relevant resistance determinants such as carbapenemases. My research also includes the development of antimicrobial, chemokine-derived peptides for use as immunostimulatory-antibiotic peptides to treat infections caused by multidrug-resistant bacterial pathogens. Additionally, as part of a collaborative effort, I am also working to develop genotypic / phenotypic diagnostic platforms to identify antibiotic resistance and inform empirical treatment.

Academic Information

Department
Medicine- Infectious Diseases and International Health

Primary Education

  • BS, Biotechnology, Montana State Universitynull
  • PhD, Microbiology, University of Virginianull
  • Research Fellowship, University of Coloradonull

Research Interests

Mechanisms of antimicrobial action and microbial strategies of resistance.

Research Disciplines

  • Infectious Diseases/Biodefense

Research Opportunities

  • Infectious Diseases

Selected Publications

2023

Cui, Z., Crawford, M. A., Rumble, B. A., Krogh, M. M., Hughes, M. A., & Letteri, R. A. (2023). Antimicrobial Peptide-Poly(ethylene glycol) Conjugates: Connecting Molecular Architecture, Solution Properties, and Functional Performance. ACS POLYMERS AU, 4(1), 45-55. doi:10.1021/acspolymersau.3c00026

Crawford, M. A., Lascols, C., Lomonaco, S., Timme, R. E., Fisher, D. J., Anderson, K., . . . Hughes, M. A. (2023). Enterobacterales draft genome sequences: 15 historical (1998-2004) and 30 contemporary (2015-2016) clinical isolates from Pakistan. MICROBIOLOGY RESOURCE ANNOUNCEMENTS. doi:10.1128/MRA.00163-23

Lascols, C., Cherney, B., Conley, A. B., Rishishwar, L., Crawford, M. A., Morse, S. A., . . . Sue, D. (2023). Investigation of multidrug-resistant plasmids from carbapenemase-producing Klebsiella pneumoniae clinical isolates from Pakistan. FRONTIERS IN MICROBIOLOGY, 14. doi:10.3389/fmicb.2023.1192097

2022

Crawford, M. A., Ward, A. E., Gray, V., Bailer, P., Fisher, D. J., Kubicka, E., . . . Hughes, M. A. (2022). Disparate Regions of the Human Chemokine CXCL10 Exhibit Broad-Spectrum Antimicrobial Activity against Biodefense and Antibiotic-Resistant Bacterial Pathogens. ACS INFECTIOUS DISEASES. doi:10.1021/acsinfecdis.2c00456

2021

Cui, Z., Luo, Q., Bannon, M. S., Gray, V. P., Bloom, T. G., Clore, M. F., . . . Letteri, R. A. (2021). Molecular engineering of antimicrobial peptide (AMP)-polymer conjugates. BIOMATERIALS SCIENCE, 9(15), 5069-5091. doi:10.1039/d1bm00423a

Callahan, V., Hawks, S., Crawford, M. A., Lehman, C. W., Morrison, H. A., Ivester, H. M., . . . Kehn-Hall, K. (2021). The Pro-Inflammatory Chemokines CXCL9, CXCL10 and CXCL11 Are Upregulated Following SARS-CoV-2 Infection in an AKT-Dependent Manner. VIRUSES-BASEL, 13(6). doi:10.3390/v13061062

2020

Lomonaco, S., Crawford, M. A., Lascols, C., Fisher, D. J., Anderson, K., Hodge, D. R., . . . Sharma, S. K. (2020). Draft Genome Sequences of Two Extensively Drug-Resistant Strains of Acinetobacter baumannii Isolated from Clinical Samples in Pakistan. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 9(20). doi:10.1128/MRA.00026-20

2019

Lomonaco, S., Lascols, C., Crawford, M. A., Anderson, K., Hodge, D. R., Fisher, D. J., . . . Sharma, S. K. (2019). Draft Genome Sequences of Antimicrobial-Resistant Shigella Clinical Isolates from Pakistan. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 8(30). doi:10.1128/MRA.00500-19

2018

Kim, J. -S., Liu, L., Fitzsimmons, L. F., Wang, Y., Crawford, M. A., Mastrogiovanni, M., . . . Vazquez-Torres, A. (2018). DksA-DnaJ redox interactions provide a signal for the activation of bacterial RNA polymerase. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115(50), E11780-E11789. doi:10.1073/pnas.1813572115

Tapscott, T., Kim, J. -S., Crawford, M. A., Fitzsimmons, L., Liu, L., Jones-Carson, J., & Vazquez-Torres, A. (2018). Guanosine tetraphosphate relieves the negative regulation of Salmonella pathogenicity island-2 gene transcription exerted by the AT-rich ssrA discriminator region. SCIENTIFIC REPORTS, 8. doi:10.1038/s41598-018-27780-9

Crawford, M. A., Margulieux, K. R., Singh, A., Nakamoto, R. K., & Hughes, M. A. (2019). Mechanistic insights and therapeutic opportunities of antimicrobial chemokines. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 88, 119-128. doi:10.1016/j.semcdb.2018.02.003

Lomonaco, S., Crawford, M. A., Lascols, C., Timme, R. E., Anderson, K., Hodge, D. R., . . . Sharma, S. K. (2018). Resistome of carbapenem-and colistin-resistant Klebsiella pneumoniae clinical isolates. PLOS ONE, 13(6). doi:10.1371/journal.pone.0198526

2017

Crawford, M. A., Fisher, D. J., Leung, L. M., Lomonaco, S., Lascols, C., Cannatelli, A., . . . Hughes, M. A. (2017). CXC Chemokines Exhibit Bactericidal Activity against Multidrug-Resistant Gram-Negative Pathogens. MBIO, 8(6). doi:10.1128/mBio.01549-17

2016

Crawford, M. A., Timme, R., Lomonaco, S., Lascols, C., Fisher, D. J., Sharma, S. K., . . . Hughes, M. A. (2016). Genome Sequences of Multidrug-Resistant, Colistin-Susceptible and-Resistant Klebsiella pneumoniae Clinical Isolates from Pakistan. GENOME ANNOUNCEMENTS, 4(6). doi:10.1128/genomeA.01419-16

Crawford, M. A., Tapscott, T., Fitzsimmons, L. F., Liu, L., Reyes, A. M., Libby, S. J., . . . Vazquez-Torres, A. (2016). Redox-Active Sensing by Bacterial DksA Transcription Factors Is Determined by Cysteine and Zinc Content. MBIO, 7(2). doi:10.1128/mBio.02161-15

Crawford, M. A., Henard, C. A., Tapscott, T., Porwollik, S., McClelland, M., & Vazquez-Torres, A. (2016). DksA-Dependent Transcriptional Regulation in Salmonella Experiencing Nitrosative Stress. FRONTIERS IN MICROBIOLOGY, 7. doi:10.3389/fmicb.2016.00444

2014

Henard, C. A., Tapscott, T., Crawford, M. A., Husain, M., Doulias, P. -T., Porwollik, S., . . . Vazquez-Torres, A. (2014). The 4-cysteine zinc-finger motif of the RNA polymerase regulator DksA serves as a thiol switch for sensing oxidative and nitrosative stress. MOLECULAR MICROBIOLOGY, 91(4), 790-804. doi:10.1111/mmi.12498

2011

Crawford, M. A., Lowe, D. E., Fisher, D. J., Stibitz, S., Plaut, R. D., Beaber, J. W., . . . Hughes, M. A. (2011). Identification of the bacterial protein FtsX as a unique target of chemokine-mediated antimicrobial activity against Bacillus anthracis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(41), 17159-17164. doi:10.1073/pnas.1108495108

2010

Crawford, M. A., Burdick, M. D., Glomski, I. J., Boyer, A. E., Barr, J. R., Mehrad, B., . . . Hughes, M. A. (2010). Interferon-Inducible CXC Chemokines Directly Contribute to Host Defense against Inhalational Anthrax in a Murine Model of Infection. PLOS PATHOGENS, 6(11). doi:10.1371/journal.ppat.1001199