Nicholas E. Sherman

Associate Professor of Research, Microbiology, Immunology, and Cancer Biology

Primary Location

PO Box 800734

Primary Phone Number

434.924.2356

Bio & Overview

Peptides and proteins are coded for by genes but are often post-translationally modified at their termini or on specific amino acid side chains. These different 'chemical species' are responsible for the wide variety of functions in living organisms. In addition, the relationship between gene expression and protein expression is often poor. Mass spectrometry is a unique technique that can measure precise masses to determine the primary sequence of a protein (identification) and the modifications (relating function). Current work focuses on the identification of proteins at sub-microgram levels (silver stain and lower) and the characterization of post-translational modifications leading to function. This task primarily starts with high quality 2D gels followed by digestion and mass spectrometry. Other methods are being explored to work on complex mixtures of proteins without 2D gel separation (2D HPLC, ICAT, etc.).

Academic Information

Department: Microbiology, Immunology, and Cancer Biology

Academic Title: Associate Professor

Primary Education

BS, Chemistry, Bridgewater College, 1990
PhD, Chemistry, University of Virginia, 1995

Other Affiliations

Co-Resource Director, Biomolecular Analysis Facility, Biomolecular Analysis Facility
Graduate Student, Chemistry

Research Interests

Mass spectrometry and related techniques to elucidate primary protein sequence and post-translational modifications

Selected Publications

2022

Dillard, L. R., Wase, N., Ramakrishnan, G., Park, J. J., Sherman, N. E., Carpenter, R., . . . Papin, J. A. (2022). Leveraging metabolic modeling to identify functional metabolic alterations associated with COVID-19 disease severity. METABOLOMICS, 18(7). doi:10.1007/s11306-022-01904-9

Lee, H. -S., Sun, H., Lagarrigue, F., Kim, S. H. J., Fox, J. W., Sherman, N. E., . . . Ginsberg, M. H. (2022). Phostensin enables lymphocyte integrin activation and population of peripheral lymphoid organs. JOURNAL OF EXPERIMENTAL MEDICINE, 219(8). doi:10.1084/jem.20211637

2021

Gimenes, S. N. C., Sachett, J. A. G., Colombini, M., Freitas-de-Sousa, L. A., Ibiapina, H. N. S., Costa, A. G., . . . Fox, J. W. (2021). Observation of Bothrops atrox Snake Envenoming Blister Formation from Five Patients: Pathophysiological Insights. TOXINS, 13(11). doi:10.3390/toxins13110800

Yang, C. -S., Jividen, K., Kamata, T., Dworak, N., Oostdyk, L., Remlein, B., . . . Paschal, B. M. (2021). Androgen signaling uses a writer and a reader of ADP-ribosylation to regulate protein complex assembly. NATURE COMMUNICATIONS, 12(1). doi:10.1038/s41467-021-23055-6

2020

Belmont-Monroy, L., Saitz-Rojas, W., Soria-Bustos, J., Mickey, A. S., Sherman, N. E., Orsburn, B. C., . . . Santiago, A. E. (2020). Characterization of a novel AraC/XylS-regulated family of N-acyltransferases in pathogens of the order Enterobacterales. PLOS PATHOGENS, 16(8). doi:10.1371/journal.ppat.1008776

2019

Wang, F., Cvirkaite-Krupovic, V., Kreutzberger, M. A. B., Su, Z., de Oliveira, G. A. P., Osinski, T., . . . Egelman, E. H. (2019). An extensively glycosylated archaeal pilus survives extreme conditions. NATURE MICROBIOLOGY, 4(8), 1401-1410. doi:10.1038/s41564-019-0458-x

Akande, O. E., Damle, P. K., Pop, M., Sherman, N. E., Szomju, B. B., Litovchick, L. V., & Grossman, S. R. (2019). DBC1 Regulates p53 Stability via Inhibition of CBP-Dependent p53 Polyubiquitination. CELL REPORTS, 26(12), 3323-+. doi:10.1016/j.celrep.2019.02.076

Du, T. -T., Dewey, J. B., Wagner, E. L., Cui, R., Heo, J., Park, J. -J., . . . Shin, J. -B. (2019). LMO7 deficiency reveals the significance of the cuticular plate for hearing function. NATURE COMMUNICATIONS, 10. doi:10.1038/s41467-019-09074-4

2018

Oostdyk, L. T., Shank, L., Jividen, K., Dworak, N., Sherman, N. E., & Paschal, B. M. (2019). Towards improving proximity labeling by the biotin ligase BirA. METHODS, 157, 66-79. doi:10.1016/j.ymeth.2018.11.003

2017

Yang, C. -S., Jividen, K., Spencer, A., Dworak, N., Ni, L., Oostdyk, L. T., . . . Paschal, B. M. (2017). Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9. MOLECULAR CELL, 66(4), 503-+. doi:10.1016/j.molcel.2017.04.028

2016

Ghazanfar, S., Fatima, I., Aslam, M., Musharraf, S. G., Sherman, N. E., Moskaluk, C., . . . Sadaf, S. (2017). Identification of actin beta-like 2 (ACTBL2) as novel, upregulated protein in colorectal cancer. JOURNAL OF PROTEOMICS, 152, 33-40. doi:10.1016/j.jprot.2016.10.011

Braun, T., Vos, M. R., Kalisman, N., Sherman, N. E., Rachel, R., Wirth, R., . . . Egelman, E. H. (2016). Archaeal flagellin combines a bacterial type IV pilin domain with an Ig-like domain. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113(37), 10352-10357. doi:10.1073/pnas.1607756113

Zelanis, A., Menezes, M. C., Kitano, E. S., Liberato, T., Tashima, A. K., Pinto, A. F. M., . . . Serrano, S. M. T. (2016). Proteomic identification of gender molecular markers in Bothrops jararaca venom. JOURNAL OF PROTEOMICS, 139, 26-37. doi:10.1016/j.jprot.2016.02.030

2015

Kawahara, R., Meirelles, G. V., Heberle, H., Domingues, R. R., Granato, D. C., Yokoo, S., . . . Paes Leme, A. F. (2015). Integrative analysis to select cancer candidate biomarkers to targeted validation. ONCOTARGET, 6(41), 43635-43652. doi:10.18632/oncotarget.6018

Suryavathi, V., Panneerdoss, S., Wolkowicz, M. J., Shetty, J., Sherman, N. E., Flickinger, C. J., & Herr, J. C. (2015). Dynamic Changes in Equatorial Segment Protein 1 (SPESP1) Glycosylation During Mouse Spermiogenesis. BIOLOGY OF REPRODUCTION, 92(5). doi:10.1095/biolreprod.114.121095

Krey, J. F., Sherman, N. E., Jeffery, E. D., Choi, D., & Barr-Gillespie, P. G. (2015). The proteome of mouse vestibular hair bundles over development. SCIENTIFIC DATA, 2. doi:10.1038/sdata.2015.47

2014

Reddy, L. A., Mikesh, L., Moskulak, C., Harvey, J., Sherman, N., Zigrino, P., . . . Fox, J. W. (2014). Host Response to Human Breast Invasive Ductal Carcinoma (IDC) as Observed by Changes in the Stromal Proteome. JOURNAL OF PROTEOME RESEARCH, 13(11), 4739-4751. doi:10.1021/pr500620x

Paige, M., Wang, K., Burdick, M., Park, S., Cha, J., Jeffery, E., . . . Shim, Y. M. (2014). Role of Leukotriene A₄ Hydrolase Aminopeptidase in the Pathogenesis of Emphysema. JOURNAL OF IMMUNOLOGY, 192(11), 5059-5068. doi:10.4049/jimmunol.1400452

Granato, D. C., Zanetti, M. R., Kawahara, R., Yokoo, S., Domingues, R. R., Aragao, A. Z., . . . Paes Leme, A. F. (2014). Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model. PLOS ONE, 9(5). doi:10.1371/journal.pone.0098208

Tirloni, L., Reck, J., Soares Terra, R. M., Martins, J. R., Mulenga, A., Sherman, N. E., . . . Vaz, I. D. S. J. (2014). Proteomic Analysis of Cattle Tick Rhipicephalus (Boophilus) microplus Saliva: A Comparison between Partially and Fully Engorged Females. PLOS ONE, 9(4). doi:10.1371/journal.pone.0094831

Linford, A. S., Jiang, N. M., Edwards, T. E., Sherman, N. E., Van Voorhis, W. C., Stewart, L. J., . . . Petri, W. A. J. (2014). Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 193(1), 33-44. doi:10.1016/j.molbiopara.2014.01.003

2013

Krey, J. F., Wilmarth, P. A., Shin, J. -B., Klimek, J., Sherman, N. E., Jeffery, E. D., . . . Barr-Gillespie, P. C. (2014). Accurate Label-Free Protein Quantitation with High- and Low-Resolution Mass Spectrometers. JOURNAL OF PROTEOME RESEARCH, 13(2), 1034-1044. doi:10.1021/pr401017h

Lu, G., Su, G., Zhao, Y., Johnston, W. F., Sherman, N. E., Rissman, E. F., . . . Upchurch, G. R. J. (2014). Dietary phytoestrogens inhibit experimental aneurysm formation in male mice. JOURNAL OF SURGICAL RESEARCH, 188(1), 326-338. doi:10.1016/j.jss.2013.11.1108

Winck, F. V., Belloni, M., Pauletti, B. A., Zanella, J. D. L., Domingues, R. R., Sherman, N. E., & Paes Leme, A. F. (2014). Phosphoproteome analysis reveals differences in phosphosite profiles between tumorigenic and non-tumorigenic epithelial cells. JOURNAL OF PROTEOMICS, 96, 67-81. doi:10.1016/j.jprot.2013.10.039

Pullikuth, A. K., Ozdemir, A., Cardenas, D., Bailey, E., Sherman, N. E., Pfister, K. K., & Catling, A. D. (2013). Epidermal Growth Factor Stimulates Extracellular-Signal Regulated Kinase Phosphorylation of a Novel Site on Cytoplasmic Dynein Intermediate Chain 2. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 14(2), 3595-3620. doi:10.3390/ijms14023595

Shin, J. -B., Krey, J. F., Hassan, A., Metlagel, Z., Tauscher, A. N., Pagana, J. M., . . . Barr-Gillespie, P. G. (2013). Molecular architecture of the chick vestibular hair bundle. NATURE NEUROSCIENCE, 16(3), 365-374. doi:10.1038/nn.3312

2012

Aragao, A. Z. B., Nogueira, M. L. C., Granato, D. C., Simabuco, F. M., Honorato, R. V., Hoffman, Z., . . . Paes Leme, A. F. (2012). Identification of Novel Interaction between ADAM17 (a Disintegrin and Metalloprotease 17) and Thioredoxin-1. JOURNAL OF BIOLOGICAL CHEMISTRY, 287(51), 43071-43082. doi:10.1074/jbc.M112.364513

Grigera, P. R., Ma, L., Borgman, C. A., Pinto, A. F., Sherman, N. E., Parsons, J. T., & Fox, J. W. (2012). Mass spectrometric analysis identifies a cortactin-RCC2/TD60 interaction in mitotic cells. JOURNAL OF PROTEOMICS, 75(7), 2153-2159. doi:10.1016/j.jprot.2012.01.012

Ali, I. K. M., Haque, R., Siddique, A., Kabir, M., Sherman, N. E., Gray, S. A., . . . Petri, W. A. J. (2012). Proteomic Analysis of the Cyst Stage of Entamoeba histolytica. PLOS NEGLECTED TROPICAL DISEASES, 6(5). doi:10.1371/journal.pntd.0001643

Tilghman, R. W., Blais, E. M., Cowan, C. R., Sherman, N. E., Grigera, P. R., Jeffery, E. D., . . . Parsons, J. T. (2012). Matrix Rigidity Regulates Cancer Cell Growth by Modulating Cellular Metabolism and Protein Synthesis. PLOS ONE, 7(5). doi:10.1371/journal.pone.0037231

2011

Kim, J., Sherman, N. E., Fox, J. W., & Ginsberg, M. H. (2011). Phosphorylation sites in the cerebral cavernous malformations complex. JOURNAL OF CELL SCIENCE, 124(23), 3929-3932. doi:10.1242/jcs.095471

Zelanis, A., Tashima, A. K., Pinto, A. F. M., Leme, A. F. P., Stuginski, D. R., Furtado, M. F., . . . Serrano, S. M. T. (2011). Bothrops jararaca venom proteome rearrangement upon neonate to adult transition. PROTEOMICS, 11(21), 4218-4228. doi:10.1002/pmic.201100287

Falick, A. M., Lane, W. S., Lilley, K. S., MacCoss, M. J., Phinney, B. S., Sherman, N. E., . . . Yates, N. A. (2011). ABRF-PRG07: advanced quantitative proteomics study.. Journal of biomolecular techniques : JBT, 22(1), 21-26.

Friedman, D. B., Andacht, T. M., Bunger, M. K., Chien, A. S., Hawke, D. H., Krijgsveld, J., . . . Turck, C. W. (2011). The ABRF Proteomics Research Group Studies: Educational exercises for qualitative and quantitative proteomic analyses. PROTEOMICS, 11(8), 1371-1381. doi:10.1002/pmic.201000736

Rao, J., Damron, F. H., Basler, M., DiGiandomenico, A., Sherman, N. E., Fox, J. W., . . . Goldberg, J. B. (2011). Comparisons of two proteomic analyses of non-mucoid and mucoid Pseudomonas aeruginosa clinical isolates from a cystic fibrosis patient. FRONTIERS IN MICROBIOLOGY, 2. doi:10.3389/fmicb.2011.00162

McIntire, W. E., Wang, D. B., Sherman, N., Shannon, J., Leonhardt, S., Yeager, M., . . . Garrison, J. C. (2011). Specific association of β₄γ₅ to adenosine A₁ and A_2A receptors determined by stable isotope labeling with heavy amino acids in cell culture and mass spectrometry. FASEB JOURNAL, 25. Retrieved from https://www.webofscience.com/

2010

Wang, D. B., Sherman, N. E., Shannon, J. D., Leonhardt, S. A., Mayeenuddin, L. H., Yeager, M., & McIntire, W. E. (2011). Binding of β₄γ₅ by Adenosine A₁ and A_2A Receptors Determined by Stable Isotope Labeling with Amino Acids in Cell Culture and Mass Spectrometry. BIOCHEMISTRY, 50(2), 207-220. doi:10.1021/bi101227y

Castro, C., Fox, J., Reuther, R., Shannon, J., Sherman, N. E., & Farmar, J. (2010). The Mass Spectrometry Core Laboratory of the Biomolecular Research Facility at the University of Virginia Health System. Journal of biomolecular techniques : JBT, 21(3 Suppl), S73.

Zelanis, A., Tashima, A. K., Furtado, M. F., Ho, P. L., Pinto, A. F., Sherman, N. E., . . . Serrano, S. M. (2010). Bothrops Jararaca Venom: Insights into the Ontogenetic Variation from Pharmacological and Proteomic Approaches. Journal of biomolecular techniques : JBT, 21(3 Suppl), S52.