Protocols

Instruction on how to use the autoclave

1. First make sure if Jacket is on (it will be showing on the screen on the top right corner). If it is off, press the jacket bottom.
2. Press number 1 to select cycle.
3. Select the cycle you wish (2 for dry or 3 for fluid).
4. Press 9 to exit the next 4 screens. These screens are showing the cycle details. Make sure you come back for the Main menu screen.
5. Press the run bottom.
6. Enter our lab room number 4062 and press enter.
7. The run should start and the receipt should start to print.
8. Write down in the logbook located on the top of the autoclave control.

Instructions on how to change CO2 tank

 Material needed:

• Big wrench (find it in the tool cabinet in room 4062)
• CO₂ tank cart (find it in the storage room – 4082)

1. Close the valve (turn it clockwise).
2. With a wrench unscrew the regulator.
3. Place the CO₂ tank “cap” back on and screw in place.
4. Place a piece of tape on the tank with the word “Empty.”
5. Remove belt and take the empty tank to the empty tanks location in the freezer room (4032) using the tank cart.
6. Using the CO₂ tank cart transfer a new CO₂ tank to the tissue culture incubator. Place the full tank next to the incubator. Make sure to fasten the belt.
7. Remove the CO₂ tank “cap.”
8. Re-attach regulator to the valve of the new tank. Screw it tightly using the wrench.
9. Open valve with a half turn counter-clockwise.
10. Check regulator to ensure adequate pressure (10 psi on the left screen and around 1000 psi on the right screen).
11. Write down when the CO₂ tank was changed on the log sheet located on the front of the incubator.

Instruction on how to sanitize the PURELAB ultra water system

Material needed:

• CT3 tablets (you will find it in the cabinet under the PURELAB ultra water system)

1. Turn the PURELAB ultra off (left side bottom).
2. Unscrew the filter that is connected to the hose. This will enable you to open the door.
3. Open the blue front door.
4. Remove the 2 cartridges by pulling up and back. Pay attention from each slot you are taking each cartridge out.
5. Open the back of one sanitization block and put one tablet inside. You will find the sanitization block attached in the inside part of the door.
6. Place the sanitization block containing the tablet in the slot of the left cartridge (slot 1).
7. Place the other sanitization block without the tablet in the slot of the right cartridge (slot 2).
8. Close the door.
9. Turn the PURELAB ultra back on.
10. Click on the check marks until sanitization starts. This will take about 54 minutes.
11. Once is done the machine will beep.
12. Turn off the PURELAB ultra again.
13. Open the front door again and remove the sanitization blocks. Put them back in place (attached to the inside part of the door).
14. Place the cartridges back. Make sure you place them in the same slot that they were before.
15. Close the door and turn the machine back on.
16. Click on the check marks. Now the PURELAB ultra water system is ready to use.