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Research Cores at University of Virginia School of Medicine
Genetically Engineered Murine Model (GEMM) Core
Home
Core Description
Lab Contact and Description
Advisory Committee
The UVa CRISPR and Transgenic Workshop
Mouse Poem by Robert Burns
Acknowledging GEMM
Flowcharts for Transgenic & Knockout Experiments
Services
Transgenic Mouse
Transgene Requirements
Transgene Injection Flowchart
Transgene Detection Methods
Gene Targeted Mouse
Mouse strains for gene targeting
Flow Chart of Targeting Experiment
From chimera to homozygous null
Congenic Mouse
Genetic background
From 129 to B6 Congenic
Backcrossing flowchart
Embryonic Stem Cell
Designing a Targeting Vector for Proper Detection
Gene Targeting Service
Karyotyping
Cryopreservation
Mouse in vitro Fertilization (IVF)
Services and Fees
CRISPR
Resource
Cre Flox Flp Mice
All Mice Considered
Links to useful mouse resources
Mice Strains Used in GEMM
Mouse Husbandry
Of Mice and Genes
Pattern for ear punch numbering system
Transgene nomenclature
Protocols
DNA purification for PCR
Yolk Sac DNA Purification Protocol
Tail DNA Purification Protocol
Transgene Detection Testing
Copy Standard Controls
Calculation of Copy Standards
Calculation of Copy Standards for PCR
PCR Using Copy Standards, Example
Southern Blot Using Copy Standards, Example
Southern Blot Protocols
DNA purification for PCR
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Services
Cryopreservation
Services and Fees
Services
Transgenic Mouse
Gene Targeted Mouse
Congenic Mouse
Embryonic Stem Cell
Services and Fees
Cryopreservation
NIH NCI Support
This core is supported by the University of Virginia Cancer Center’s National Cancer Institute P30 Center Grant.
CRISPR Workshop
The UVa CRISPR and Transgenic Workshop is held two times a year. You can register for the Spring or Fall workshop on line.