Services
Please submit requests in iLAB for services of GEMM Core. Fees are approved by the institution and are listed below FYI. UVa Cancer Center members should contact CC for competing funds allocated to support for the use of CC shared resources. Non-UVa users are encouraged to contact us for terms and fees.
| Service name as in iLab | Description | Fee |
|---|---|---|
Microinjection and Electroporation |
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| CRISPR – B6SJL | single day reagent delivery for CRISPR gene editing | $1,750 |
| CRISPR – B6/other strains | single day reagent delivery for CRISPR gene editing | $3,000 |
| DNA, RNA, protein delivery -B6SJL | per construct with 3 founder guarantee for traditional transgenesis, recommended for CRISPR KI projects with multiple injection/electroporation guaranteed | $4,850 |
| DNA, RNA, protein delivery -B6/other strains | per construct with 3 founder guarantee for traditional transgenesis, recommended for CRISPR KI projects with multiple injection/electroporation guaranteed | $6,200 |
| Blastocyst/morula injection | per clone with >50 blastocyst/morula injected | $5,850 |
| CRISPR – cell | per cell line gene editing | $5,000 |
Embryonic Stem Cells |
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| ESC expansion | 4 vials with 2 million cells each | $525 |
| ESC for targeting or DNA prep | 1 vial for targeting/5 vials for DNA prep | $75 |
| PMEF derivation | 3 million cells/per vial (20 vials minimum per genotype) | $69 |
Cryopreservation and Rederivation |
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| Embryo cryo-100 | 100 embryos with test thaw | $1,500 |
| Embryo cryo-200 | 200 embryos with test thaw | $2,600 |
| Embryo cryo-300 | 300 embryos with test thaw | Request |
| Sperm Cryo | 10 straws of frozen sperm from 2 males | $700 |
| Embryo implantation | up to 3 ICR recipient female mice | $655 |
| IVF | >100 eggs + sperm from 1 male or 1 vial | $1,550 |
| Customized breeding | 12 cages per month | $310 |