Luca Musante, PhD

PRIMARY APPOINTMENT:

Assistant Professor of Research

CONTACT:

UVA Division of Nephrology
PO Box 800133
Charlottesville, VA 22908
Telephone: 434-924-5125
Email: lm8qd@virginia.edu

EDUCATION AND TRAINING:

  • 2005: PhD in Experimental Medicine, University of Modena and Reggio Emilia, Modena, Italy
  • 2000: Specialist in Biochemistry and Clinical Chemistry cum Laude, University of Pavia, Pavia, Italy
  • 1994: Master’s Degree in Biological Sciences, University of Genova, Genoa, Italy

RESEARCH AREAS:

Biomarkers, extracellular vesicles, biochemistry and protein analysis

Lab Images
  • Luca Musante, PhD

  • Luca Musante, PhD

  • Luca Musante, PhD

RESEARCH SUMMARY:

Over the last 10 years, my attention has focused on the analysis of extracellular vesicles (EVs) as new sources of biomarkers for early detection and investigation of damage to the kidney due to diabetes. I joined Dr Erdbrügger laboratory in 2016 to investigate the role of plasma and urinary extracellular vesicles in hypertension. Not only have EVs become a source of new diagnostic markers, but it is also now agreed that they represent a new cell-to-cell communication system with unlimited potential to develop new therapeutic strategies aimed to achieve tissue regeneration and repair of damage caused by any primary disease. The study of biomarkers relevant in kidney damage due to hypertension and other renal diseases in humans and corresponding animal models of disease has become of paramount importance. An early diagnosis following the first signs of kidney imparment is badly needed to intervene in order to delay the progression of the injury. In parallel, the analysis of urinary extracellular vesicles (uEVs) and plasma extracellular vesicles (pEvs) requires a costant improvement of experimental design and techniques such as tunable resistive pulse sensing (TRSP), nano track analysis (NTA), imaging flow cytometry and other high-sensitivity tools employed to analyze extracellular vesicles. Moreover, the expanding research to understand the physiological role of EVs calls for the need for extreme fine tuning of the biochemical purification tools of choice in order to obtain the highest possible pure preparation of extracellular vesicles by eliminating any confounding carry over of other molecules such as soluble proteins abundantly present in any biofluids. For this reason, designing new purification strategies to minimize loss of precious material from limited amounts of specimen while preserving the integrity of vesicles is another exciting and demanding stream of research currently in progress to test and identify EVs activity pathways.

PUBLICATIONS

  1. Eichner NZM, Gilbertson NM, Heiston EM, Musante L, La Salvia S, Weltman A, Erdbrugger U, Malin SK. Interval exercise lowers circulating CD105 extracellular vesicles in prediabetes. Med Sci Sports Exerc. 2019 Oct 9. doi: 10.1249/MSS.0000000000002185.
  2. Xu X, Barreiro K, Musante L, Kretz O, Lin H, Zou H, Huber TB, Holthofer H. Management of Tamm-Horsfall Protein for Reliable Urinary Analytics. Proteomics Clin Appl. 2019 Aug 19;:e1900018. doi: 10.1002/prca.201900018. [Epub ahead of print] PubMed PMID: 31424164.
  3. Mitchell R, Mellows B, Sheard J, Antonioli M, Kretz O, Chambers D, Zeuner MT, Tomkins JE, Denecke B, Musante L, Joch B, Debacq-Chainiaux F, Holthofer H, Ray S, Huber TB, Dengjel J, De Coppi P, Widera D, Patel K. Secretome of adipose-derived mesenchymal stem cells promotes skeletal muscle regeneration through synergistic action of extracellular vesicle cargo and soluble proteins. Stem Cell Res Ther. 2019 Apr 5;10(1):116. doi: 10.1186/s13287-019-1213-1. PubMed PMID: 30953537; PubMed Central PMCID: PMC6451311.
  4. Eichner NZM, Gilbertson NM, Musante L, La Salvia S, Weltman A, Erdbrügger U, Malin SK. An Oral Glucose Load Decreases Postprandial Extracellular Vesicles in Obese Adults with and without Prediabetes. Nutrients. 2019 Mar 8;11(3). doi: 10.3390/nu11030580. PubMed PMID: 30857250; PubMed Central PMCID: PMC6470527.
  5. Théry C, Witwer KW, Aikawa E, Alcaraz MJ, Anderson JD, Andriantsitohaina R, Antoniou A, Arab T, Archer F, Atkin-Smith GK, Ayre DC, Bach JM, Bachurski D, Baharvand H, Balaj L, Baldacchino S, Bauer NN, Baxter AA, Bebawy M, Beckham C, Bedina Zavec A, Benmoussa A, Berardi AC, Bergese P, Bielska E, Blenkiron C, Bobis-Wozowicz S, Boilard E, Boireau W, BongiovanniA, Borràs FE, Bosch S, Boulanger CM, Breakefield X, Breglio AM, Brennan MÁ, Brigstock DR, Brisson A, Broekman ML, Bromberg JF, Bryl-Górecka P, Buch S, Buck AH, Burger D, Busatto S, Buschmann D, Bussolati B, Buzás EI, Byrd JB, Camussi G, Carter DR, Caruso S, Chamley LW, Chang YT, Chen C, Chen S, Cheng L, Chin AR, Clayton A, Clerici SP, Cocks A, Cocucci E, Coffey RJ, Cordeiro-da-Silva A, Couch Y, Coumans FA, Coyle B, Crescitelli R, Criado MF, D’Souza-Schorey C, Das S, Datta Chaudhuri A, de Candia P, De Santana EF, De Wever O, Del Portillo HA, Demaret T, Deville S, Devitt A, Dhondt B, Di Vizio D, Dieterich LC, Dolo V, Dominguez Rubio AP, Dominici M, Dourado MR, Driedonks TA, Duarte FV, Duncan HM, Eichenberger RM, Ekström K, El Andaloussi S, Elie-Caille C, Erdbrügger U, Falcón-Pérez JM, Fatima F, Fish JE, Flores-Bellver M, Försönits A, Frelet-Barrand A, Fricke F, Fuhrmann G, Gabrielsson S, Gámez-Valero A, Gardiner C, Gärtner K, Gaudin R, Gho YS, Giebel B, Gilbert C, Gimona M, Giusti I, Goberdhan DC, Görgens A, Gorski SM, Greening DW, Gross JC, Gualerzi A, Gupta GN, Gustafson D, Handberg A, Haraszti RA, Harrison P, Hegyesi H, Hendrix A, Hill AF, Hochberg FH, Hoffmann KF, Holder B, Holthofer H, Hosseinkhani B, Hu G, Huang Y, Huber V, Hunt S, Ibrahim AG, Ikezu T, Inal JM, Isin M, Ivanova A, Jackson HK, Jacobsen S, Jay SM, Jayachandran M, Jenster G, Jiang L, Johnson SM, Jones JC, Jong A, Jovanovic-Talisman T, Jung S, Kalluri R, Kano SI, Kaur S, Kawamura Y, Keller ET, Khamari D, Khomyakova E, Khvorova A, Kierulf P, Kim KP, Kislinger T, Klingeborn M, Klinke DJ 2nd, Kornek M, Kosanović MM, Kovács ÁF, Krämer-Albers EM, Krasemann S, Krause M, Kurochkin IV, Kusuma GD, Kuypers S, Laitinen S, Langevin SM, Languino LLR, Lannigan J, Lässer C, Laurent LC, Lavieu G, Lázaro-Ibáñez E, Le Lay S, Lee MS, Lee YXF, Lemos DS, Lenassi M, Leszczynska A, Li IT, Liao K, Libregts SF, Ligeti E, Lim R, Lim SK, Linē A, Linnemannstöns K, Llorente A, Lombard CA, Lorenowicz MJ, Lörincz ÁM, Lötvall J, Lovett J, Lowry MC, Loyer X, Lu Q, Lukomska B, Lunavat TR, Maas SL, Malhi H, Marcilla A, Mariani J, Mariscal J, Martens-Uzunova ES, Martin-Jaular L, Martinez MC, Martins VR, Mathieu M, Mathivanan S, Maugeri M, McGinnis LK, McVey MJ, Meckes DG Jr, Meehan KL, Mertens I, Minciacchi VR, Möller A, Møller Jørgensen M, Morales-Kastresana A, Morhayim J, Mullier F, Muraca M, Musante L, Mussack V, Muth DC, Myburgh KH, Najrana T, Nawaz M, Nazarenko I, Nejsum P, Neri C, Neri T, Nieuwland R, Nimrichter L, Nolan JP, Nolte-‘t Hoen EN, Noren Hooten N, O’Driscoll L, O’Grady T, O’Loghlen A, Ochiya T, Olivier M, Ortiz A, Ortiz LA, Osteikoetxea X, Østergaard O, Ostrowski M, Park J, Pegtel DM, Peinado H, Perut F, Pfaffl MW, Phinney DG, Pieters BC, Pink RC, Pisetsky DS, Pogge von Strandmann E, Polakovicova I, Poon IK, Powell BH, Prada I, Pulliam L, Quesenberry P, Radeghieri A, Raffai RL, Raimondo S, Rak J, Ramirez MI, Raposo G, Rayyan MS, Regev-Rudzki N, Ricklefs FL, Robbins PD, Roberts DD, Rodrigues SC, Rohde E, Rome S, Rouschop KM, Rughetti A, Russell AE, Saá P, Sahoo S, Salas-Huenuleo E, Sánchez C, Saugstad JA, Saul MJ, Schiffelers RM, Schneider R, Schøyen TH, Scott A, Shahaj E, Sharma S, Shatnyeva O, Shekari F, Shelke GV, Shetty AK, Shiba K, Siljander PR, Silva AM, Skowronek A, Snyder OL 2nd, Soares RP, Sódar BW, Soekmadji C, Sotillo J, Stahl PD, Stoorvogel W, Stott SL, Strasser EF, Swift S, Tahara H, Tewari M, Timms K, Tiwari S, Tixeira R, Tkach M, Toh WS, Tomasini R, Torrecilhas AC, Tosar JP, Toxavidis V, Urbanelli L, Vader P, van Balkom BW, van der Grein SG, Van Deun J, van Herwijnen MJ, Van Keuren-Jensen K, van Niel G, van Royen ME, van Wijnen AJ, Vasconcelos MH, Vechetti IJ Jr, Veit TD, Vella LJ, Velot É, Verweij FJ, Vestad B, Viñas JL, Visnovitz T, Vukman KV, Wahlgren J, Watson DC, Wauben MH, Weaver A, Webber JP, Weber V, Wehman AM, Weiss DJ, Welsh JA, Wendt S, Wheelock AM, Wiener Z, Witte L, Wolfram J, Xagorari A, Xander P, Xu J, Yan X, Yáñez-Mó M, Yin H, Yuana Y, Zappulli V, Zarubova J, Žėkas V, Zhang JY, Zhao Z, Zheng L, Zheutlin AR, Zickler AM, Zimmermann P, Zivkovic AM, Zocco D, Zuba-Surma EK. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. 2018;7(1):1535750. doi: 10.1080/20013078.2018.1535750. eCollection 2018. PubMed PMID: 30637094; PubMed Central PMCID: PMC6322352.
  6. Clayton A, Buschmann D, Byrd JB, Carter DRF, Cheng L, Compton C, Daaboul G, Devitt A, Falcon-Perez JM, Gardiner C, Gustafson D, Harrison P, Helmbrecht C, Hendrix A, Hill A, Hoffman A, Jones JC, Kalluri R, Kang JY, Kirchner B, Lässer C, Lawson C, Lenassi M, Levin C, Llorente A, Martens-Uzunova ES, Möller A, Musante L, Ochiya T, Pink RC, Tahara H, Wauben MHM, Webber JP, Welsh JA, Witwer KW, Yin H, Nieuwland R. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017. J Extracell Vesicles. 2018;7(1):1473707. doi: 10.1080/20013078.2018.1473707. eCollection 2018. PubMed PMID: 31162490; PubMed Central PMCID: PMC5965025.
  7. Eichner NZM, Gilbertson NM, Gaitan JM, Heiston EM, Musante L, LaSalvia S, Weltman A, Erdbrügger U, Malin SK. Low cardiorespiratory fitness is associated with higher extracellular vesicle counts in obese adults Physiol Rep. 2018 May;6(10):e13701. doi: 10.14814/phy2.13701.
  8. Hu S, Musante L, Tataruch D, Xu X, Kretz O, Henry M, Meleady P, Luo H, Zou H, Jiang Y, Holthofer H. Purification and Identification of Membrane Proteins from Urinary Extracellular Vesicles using Triton X-114 Phase Partitioning.J Proteome Res. 2018 Jan 5;17(1):86-96. doi: 10.1021/acs.jproteome.7b00386.
  9. Doublier S, Zennaro C, Musante L, Spatola T, Candiano G, Bruschi M, Besso L, Cedrino M, Carraro M, Ghiggeri GM, Camussi G, Lupia E. Soluble CD40 ligand directly alters glomerular permeability and may act as a circulating permeability factor in FSGS. PLoS One. 2017; 12:e0188045. doi: 10.1371/journal.pone.0188045.
  10. Mellows B, Mitchell R, Antonioli M, Kretz O, Chambers D, Zeuner MT, Denecke B, Musante L, Ramachandra DL, Debacq-Chainiaux F, Holthofer H, Joch B, Ray S, Widera D, David AL, Huber TB, Dengjel J, De Coppi P, Patel K. Protein and Molecular Characterization of a Clinically Compliant Amniotic Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating Muscle Regeneration Through Enhancement of Angiogenesis. Stem Cells Dev. 2017;26:1316-1333. doi: 10.1089/scd.2017.0089.
  11. Musante L, Tataruch-Weinert D,Kerjaschki D, Henry M, Meleady P, Holthofer H. Characterization of Residual Urinary Extracellular Vesicles in Ultracentrifugation Supernatants. J Extracell Vesicles. 2016; 6:1267896. doi: 10.1080/20013078.2016.1267896.
  12. Tataruch-Weinert D, Musante L, Kretz O, Holthofer H. Urinary Extracellular Vesicles for RNA Extraction: Optimization of a Protocol Devoid of Prokaryote Contamination. J Extracell Vesicles 2016, 5:30281: doi:10.3402/jev.v5.30281.
  13. Musante L,Tataruch D, Gu D, Xinyu Liu, Forsblom C, Groop PH, Harry Holthöfer H. Proteases and Protease Inhibitors of Urinary Extracellular Vesicles in Diabetic Nephropathy. J Diabetes Res 2015: 2015:289734doi: 10.1155/2015/289734.
  14. Saraswat M, Joenvaara S, Musante L, Peltoniemi H, Holthöfer H, Renkonen R. N-glycoproteomics of urinary exosomes. Mol Cell Proteomics 2015: 14:2298 doi: 10.1074/mcp.A114.040345.
  15. Liu X, ChinelloC, MusanteL, CazzanigaMTataruchD, CalzaferriG, MagniF, ZouH, HolthöferH. Intraluminal Proteome and Peptidome of Human Urinary Extracellular Vesicles. Proteomics ClinAppl 2015: 9:568-73, doi: 10.1002/prca.201400085.
  16. Ravida A, Musante L, Kreivi M, Miinalainen I, Byrne B, Saraswat M, Henry M, Meleady, Clynes M Holthöfer H. Glycosylation patterns in rat diabetic nephropathy. Kidney Int 2015: 87: 963-74 doi: 10.1038/ki2014.387.
  17. Musante L,Tataruch D, Gu D, Benito-Martin A, Calzaferri G, Aherne S, Holthöfer H. A Simplified Method to Recover Urinary Vesicles for Clinical Applications, and Sample Banking. Scientific Reports 2014, 4, 7532; doi:10.1038/srep07532.
  18. Musante L, Tataruch DE, Holthöfer H. Use and isolation of urinary exosomes as biomarkers for diabetic nephropathy. Front Endocrinol (Lausanne) 2014 5:149.
  19. Wasik AA, Koskelainen S, Hyvonen ME, Musante L, Lehtonen E, Koskenniemi K, Tienari J, Vaheri A, Kerjaschki D, Szalay C, Revesz C, Varmanen P, Nyman TA, Hamar O, Holthöfer H, Lehtonen S. Am J Pathol 2014 184:1727-39.
  20. Musante L, Saraswat M, Ravidà A, Byrne B, Holthöfer H. Recovery of urinary nanovesicles from ultracentrifugation supernatants. Nephrol Dial Transplant. 2013 Jun;28(6):1425-33.
  21. Saraswat M, Musante L, Ravidá A, Shortt B, Byrne B, Holthöfer H. Preparative purification of recombinant proteins: current status and future trends. Biomed Res Int. 2013;2013:312709.
  22. Musante L, Saraswat M, Duriez E, Byrne B, Ravidà A, Domon B, Holthöfer H. Biochemical and physical characterisation of urinary nanovesicles following CHAPS treatment. PLoS One. 2012;7(7):e37279.
  23. Ghiggeri GM, Bruschi M, Musante L, Candiano G, Boccardi C, Citti L, Rastaldi MP, Mangraviti S, Rosso G, Larti A, Rosati A, Urbani A, Gusmano R, Bertoni E, Salvadori M. Post-transplant proteinuria associated with everolimus: Definition of main features with proteomics ProteomicsClin Appl. 2008; 2: 1327-37.
  24. Candiano G, Santucci L, Petretto A, Pavone B, Del Boccio P, Musante L, Bruschi M, Federici G, Gusmano R, Urbani A, Ghiggeri GM. High-resolution 2-DE for resolving proteins, protein adducts and complexes in plasma.Electrophoresis. 2008; 29: 682-94.
  25. Panfoli I, Musante L, Bachi A, Ravera S, Calzia D, Cattaneo A, Bruschi M, Bianchini P, Diaspro A, Morelli A, Pepe IM, Tacchetti C, Candiano G Proteomic Analysis of the Retinal Rod Outer Segment Disks. J Proteome Res. 2008; 7: 2654-2669.
  26. Candiano G, Musante L, Petretto A, Bruschi M, Santucci L, Urbani A, Scolari F, Gusmano R, Carraro M, Zennaro C, Vincenti F, Ghiggeri GM Proteomics of plasma and urine in primary nephrotic syndrome in children. Contrib Nephrol 2008; 160: 17-28.
  27. Bruschi M, Petretto A, Candiano G, Musante L, Movilli E, Santucci L, Urbani A, Gusmano R Verrina E, Cancarini G, Scolari F, Ghiggeri GM Determination of the oxido-redox status of plasma albumin in hemodialysis patients. J Chromatogr B Analyt Technol Biomed Life Sci. 2008; 864: 29-37.
  28. CandianoG, Santucci L, Petretto A, Pavone B, Del Boccio P, Musante L, Bruschi M, Federici G, Gusmano R, Urbani A, Ghiggeri GM High-resolution 2-DE for resolving proteins, protein adducts and complexes in plasma. Electrophoresis 2008; 29: 682-94.
  29. Ghiggeri GM, Musante L,Candiano G, Bruschi M, Santucci L, Barbano G, Trivelli A, Rivabella L, Gusmano R, Perfumo F Protracted remission of proteinuria after combined therapy with plasmapheresis and anti-CD20 antibodies/cyclophosphamide in a child with oligoclonal IgM and glomerulosclerosis. Pediatr Nephrol. 2007; 22:1953-1956.
  30. Ravera S, Musante L, Calzia D, Panfoli I, Bruschi M, Candiano G, Pepe IM, Morelli A. Expression of adenylate kinase 1 in bovine retinal cytosol. Curr Eye Res 2007; 32: 249-57.
  31. Bruschi M, Musante L, Ghiggeri GM, Rialdi G, Candiano G. Comparative study of thermal stability of healthy and focal segmental glomerulosclerosis plasma albumin. Journal of thermal analysis and calorimetry 2007, 27-31.
  32. Musante L, Candiano G, Petretto A Bruschi M, Dimasi N, Caridi G, Pavone B, Galliano M, Urbani A, Scolari F, Vincenti F, Ghiggeri G. Active Focal Segmental Glomerulosclerosis Is Associated with Massive Oxidation of Plasma Albumin. J Am SocNephrol. 2007; 18: 799-810.
  33. Candiano G, Bruschi M, Pedemonte N, Musante L, Ravazzolo R, Liberatori S, Bini L, Galletta LJ, Zegarra-Moran O Proteomic analysis of the airway surface liquid: modulation by proinflammatory cytokines. Am J Physiol Lung Cell Mol Physiol. 2007; 292: 185-98.
  34. Candiano G, Musante L, Bruschi M, Petretto A, Santucci L, Del Boccio P, Pavone B, Per fumo F, Urbani A, Scolari F, Ghiggeri GM. Repetitive Fragmentation Products of Albumin and {alpha}1-Antitrypsin in Glomerular Diseases Associated with Nephrotic Syndrome. J Am SocNephrol. 2006; 17: 3139-48.
  35. Conti V, Aghaie A, Cilli M, Martin N, Caridi G, Musante L, Candiano G, Castagna M, Fairen A, Ravazzoli R, Guenet JL, Puliti A crv4, a mouse model for human ataxia associated with kyphoscoliosis caused by an mRNA splicing mutation of the metabotropic glutamate receptor 1 (Grm1). Int J Mol Med. 2006; 18: 593-600.
  36. Musante L, Bruschi M, Candiano G, Petretto A, Dimasi N, Boccio PD, Urbani A, Rialdi G, Ghiggeri GM Characterization of oxidation end product of plasma albumin ‘in vivo’ BiophysBiochem Res Commun 2006; 349: 668-73.
  37. Rastaldi MP, Candiano G, Musante L, Bruschi M, Armelloni S, Rimoldi L, Tardanico R, Cherchi SS, Ferrario F, Montinaro V, Haupt R, Parodi S, Carnevali ML, Allegri L, Camussi G, Gesualdo L, Scolari F, Ghiggeri GM: Glomerular clusterin is associated with PKC-alpha/beta regulation and good outcome of membranous glomerulonephritis in humans. Kidney Int 2006; 70: 477-85.
  38. Bruschi M, Musante L, Candiano G, Santucci L, Zennaro C, Carraro M, Del Boccio P, Gusmano R, Perfumo F, Urbani A, Ghiggeri GM: Transitions of serum albumin in patients with glomerulosclerosis ‘in vivo’ characterization by electrophoretic titration curves. Electrophoresis 2006; 27: 2960-9.
  39. Marini M, Bruschi M, Pecci A, Romagnoli R, Musante L, Candiano G, Ghiggeri GM, Balduini C, Seri M, Ravazzolo R: Non-muscle myosin heavy chain IIA and IIB interact and co-localize in living cells: relevance for MYH9-related disease Int J Mol Med 2006; 17: 729-736,29.
  40. Levrieri I, Musante L, Petretto A, Bruschi M, Candiano G, Melioli G Separation of human serum proteins using the Beckman-Coulter PF2D_ system: analysis of ion exchange-based first dimension chromatography ClinChem Lab Med 2005; 43: 1327–33.
  41. Bruschi M, Seppi C, Arena S, Musante L, Santucci L, Balduini C Scaloni A, Lanciotti M, Righetti PG, Candiano G. Proteomic Analysis of Erythrocyte Membranes by Soft Immobiline Gels Combined with Differential Protein Extraction. J Proteome Res. 2005; 4: 1304-1309.
  42. Musante L, Candiano G, Bruschi M, Santucci L, Carnemolla B, Orecchia P, Gianpuzzi M, Zennaro C, Sanna-Cherchi S, Carraro M, Oleggini R, Camussi G, Perfumo F, Ghiggeri GM. Circulating anti-actin and anti-ATP synthase antibodies identify a sub-set of patients with idiopathic nephrotic syndrome. ClinExpImmunol. 2005; 141: 491-9.
  43. Doublier S, Musante L, Lupia E, Candiano G, Spatola T, Caridi G, Zennaro C, Carraro M, Ghiggeri GM, Camussi G. Direct effect of plasma permeability factors from patients with idiopatic FSGS on nephrin and podocin expression in human podocytes. Int J Mol Med. 2005 16 49-58
  44. Ghiggeri GM, Artero M, Carraro M, Candiano G, Musante L, Bruschi M, Zennaro C, Ginevri F, Caridi G, Faccini L, Perfumo F, Gusmano R. Glomerular albumin permeability as an in vitro model for characterizing the mechanism of focal glomerulosclerosis and predicting post-transplant recurrence. Pediatr Transplant 2004 8 339-43.
  45. Carraro M, Zennaro C, Artero M, Candiano G, Ghiggeri GM, Musante L, Sirch C, Bruschi M, Faccini L. The effect of proteinase inhibitors on glomerular albumin permeability induced in vitro by serum from patients with idiopathic focal segmental glomerulosclerosis. Nephrol Dial Transplant. 2004 19 1969-75.
  46. Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri GM, Carnemolla B, Orecchia P, Zardi L, Righetti PG Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis Electrophoresis 2004 25 1337-43.
  47. Bruschi M, Musante L, Candiano G, Ghiggeri GM, Herbert B, Antonucci F, Righetti PG Soft immobilized pH gradient gels in proteome analysis: A follow-up. Proteomics 2003 3 821-5.
  48. Carraro M, Zennaro C, Candiano G, Musante L, Bruschi M, Ghiggeri GM, Artero M, Faccini L. Nephrotic urine prevents increased rat glomerular albumin permeability induced by serum from the same patient with idiopathic nephrotic syndrome. Nephrol Dial Transplant. 2003 18 689-93.
  49. Bruschi M, Catarsi P, Candiano G, Rastaldi MP, Musante L, Scolari F, Artero M, Carraro M, Carrea A, Caridi G, Zennaro C, Sanna-Cherchi S, Viola FB, Ferrario F, Perfumo F, Ghiggeri GM Apolipoprotein E in idiopathic nephrotic syndrome and focal segmental glomerulosclerosis. Kidney Int. 2003 63 686-695.
  50. Ghiggeri GM, Bruschi M, Candiano G, Rastaldi MP, Scolari F, Passerini P, Musante L, Pertica N, Caridi GL, Ferrario F, Perfumo F, Ponticelli C Depletion of clusterin in renal diseases causing nephrotic syndrome. Kidney International 2002 62 2184-2194.
  51. Carraro M, Caridi G, Bruschi M, Artero M, Bertelli R, Zennaro C, Musante L, Candiano G, Perfumo F, Ghiggeri GM Serum Glomerular Permeability Activity in Patients with Podocin Mutations (NPHS2) and Steroid-ResistantNephroticSyndrome.J Am SocNephrol. 2002 13:1946-52.
  52. Musante L, Candiano G, Bruschi M, Zennaro C, Carraro M, Artero M, Giuffrida MG, Conti A, Santucci A, Ghiggeri GM Characterization of plasma factors that alter the permeability to albumin within isolate glomeruli Proteomics 2002 2: 197-205.
  53. Candiano G, Musante L, Bruschi M, Ghiggeri GM, Herbert B, Antonucci F, Righetti PG Two-dimensional maps in soft IPG gels: a new approach to the proteome of the third millennium Electrophoresis 2002 23: 292-97.
  54. Musante L, Candiano G, Zennaro C, Bruschi M, Carraro M, Aretero M, Ghiggeri GM Humoral permeability factors in the nephrotic syndrome: a compendium and prospectus. J Nephrol. 2001 14: 48-50.
  55. Caridi GL, Bertelli R, Carrea A, Di Duca M, Catarsi P, Artero M, Carraro M, Zennaro C, Candiano G, Musante L, Seri M, Ginevri F, Perfumo F, Ghiggeri GM Prevalence genetics and clinical features of patients carrying podocin mutations in steroid resistant non familial focal segmental glomerulosclerosis. J Am SocNephrol 2001 12: 2742-6.
  56. Candiano G, Musante L, Zennaro C, Bruschi M, Carraro M, Artero M, Gusmano R, Ginevri F, Perfumo F, Ghiggeri GM Inhibition of renal permeability towards albumin: a new function of apolipoproteins with possible pathogenetic relevance in focal glomerulosclerosis. Electrophoresis. 2001 22; 1819-25.
  57. Candiano G, Musante L, Carraro M, Faccini L, Campanacci L, Zennaro C, Artero M, Ginevri F, Perfumo F, Gusmano R, Ghiggeri GM. Apolipoproteins prevent glomerular albumin permeability induced in vitro by serum from patients with focal segmental glomerulosclerosis. J Am SocNephrol. 2001; 12: 143-50.
  58. Ghiggeri GM, Oleggini R, Musante L, Caridi G, Gusmano R, Ravazzolo R. A DNA element in the alpha1 type III collagen promoter mediates stimulatory response by angiotensin II. Kidney Int. 2000; 58: 537-48.
  59. Oleggini R, Musante L, Menoni S, Botti G, Duca MD, Prudenziali M, Carrea A, Ravazzolo R, Ghiggeri GM. Characterization of a DNA binding site that mediates the stimulatory effect of cyclosporin on Type III collagen expression in renal cells. Nephrol Dial Transplant. 2000; 15: 778-85
  60. Attanasio F, Bruschi M, Candiano G, Galletto R, Musante L, Schulein M, Rialdi G. Analytical titration curves of glycosyl Cel45 by combined isoelectric focusing-electrophoresis. Electrophoresis. 1999; 20: 1403-11.
  61. Musante L, Ulivi M, Cutrona G, Chiorazzi N, Roncella S, Candiano G, Ferrarini M. Identification of HSP-60 as the specific antigen of IgM produced by BRG-lymphoma cells. Electrophoresis. 1999; 20: 1092-7.
  62. Ginevri F, Gusmano R, Oleggini R, Valenti F, Botti G, Musante L, Candiano G, Ravazzolo R, Ghiggeri GM. Activation of COL3A1 promoter activity by cyclosporine. Transplant Proc. 1998; 30: 955-6.
  63. Balestrino M, Cupello A, Morelli A, Musante L, Panfoli I. Measurements of Na+/K+- ATPase after in vitro hypoxia and reoxgenation are affected by methods of membrane preparation. J Neurosci Methods. 1998; 79: 201-6.
  64. Musante L, Candiano G, Ghiggeri GM. Resolution of fibronectin and other uncharacterised proteins by two-dimensional polyacrylamide electrophoresis with thiourea. J Chromatogr B Biomed Sci Appl. 1998; 705: 351-6.
  65. Panfoli I, Musante L, Morelli A, Thellung S, Cupello A. Ca2+-ATPase pump forms and an endogenous inhibitor in bovine brain synaptosomes. Neurochem Res. 1997; 22: 297-304.