Genetic and Epigenetic stability in human cancer
Epigenetic changes accompany an individual’s development, aging, and disease. They’re particularly significant markers for cancer’s onset and progression, posing substantial therapeutic challenges. Increasing evidence suggests that DNA replication stress leads to these epigenetic alterations. Epigenetic changes at the replication fork can impact its repair and genome stability. Our goal is to understand the relationship between DNA replication stress responses and epigenetic changes, shedding light on tumor mechanisms and reasons for drug resistance.
Building upon traditional biochemical techniques, we employ various proteomic methods for DNA replication forks combined with single-molecule immunofluorescence, and high-throughput sequencing to study epigenetics at the stalled replication fork. The CRISPR-Cas9 system, alongside rapid protein degradation techniques (AID2), is used to manipulate target proteins.
Wenpeng Liu, Ph.D.
Assistant Professor of Radiation Oncology
University of Virginia School of Medicine
Lab Room 7121, Multistory Building, West Complex, PO Box 800383
Lab Website: https://dnas.bio/