The Redemann Lab is interested in uncovering and understanding the underlying principles of spindle assembly, the structure function relation and the basics of the huge variability of spindle size, architecture and mechanics between different tissues as well as different species. In particular we are very interested in the adaptation of spindle architecture and function during cell differentiation.

We are using a combination of large scale 3D reconstruction of spindles by electron tomography and state-of-the-art light microscopy to investigate the mechanisms and principles of spindle assembly and chromosome segregation. Ultimately we are using the dynamic and ultra structural data to develop and test models of spindle formation and mechanics.

3D reconstruction of a metaphase spindle of the C. elegans one-cell embryo

Mitotic spindles are highly dynamic, microtubule based constructions that function to segregate the chromosomes during cell division. The dynamic properties of microtubules in spindles are modulated by many factors, including polymerases, depolymerases, motor proteins, cross-linkers and other microtubule associated proteins, of which many are conserved throughout eukaryotic organisms. Despite this evolutionary conservation of essential factors, there is a remarkable variability in spindle organization and mechanics between organisms and tissues within species.